Objective: To establish an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of twenty chemical components(arecoline hydrobromide, adenosine cyclophosphate, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, liquiritin, benzoylmesaconine, benzoylaconitine, isoliquiritin, benzoylhypacoitine, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, 6-gingerol, honokiol, costunolide, dehydrocostus lactone, magnolol, betulinic acid)in Shipi powder. Methods: The separation was performed over 40.0 min on a Shim-pack GIST C18 column(100 mm×2.1 mm,2.0 μm) with a mobile phase consisting of 0.1% formic acid in water and acetonitrile with gradient elution (0-12 min, 2%B→14%B;12-24 min,14%B→51%B;24-26 min,51%B→70%B;26-36 min,70%B;36-37 min,70%B→2%B;37-40 min,2%B) at a flow rate of 0.3 mL·min-1. The column temperature was 35 ℃ and the injection volume was 1 μL. The detection was carried out by electrospray ionization(ESI). Positive and negative electrospray ionizations were performed in multiple reaction monitoring(MRM) mode. Results: All of the analytes showed good linearity(r≥0.997 6) in the tested ranges. The precision, repeatability and stability of the method were good for the twenty chemical components. The average recoveries were in the range of 93.4%-108.7% with relative standard deviations RSD≤5.0%. The contents of arecoline, adenosine cyclophosphate, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, liquiritin, benzoylmesaconine, benzoylaconitine, isoliquiritin, benzoylhypacoitine, liquiritigenin, isoliquiritigenin, glycyrrhizic acid, 6-gingerol, honokiol, costunolide, dehydrocostus lactone, magnolol, betulinic acid in 15 bantches of in Shipi powder were 7 190.26-13 726.74, 90.11-301.93, 9.65-71.13, 102.88-710.98, 40.51-179.73, 10.90-71.22, 3 424.43-27 337.63, 508.34-1 276.14, 108.27-192.65, 95.74-378.92, 565.19-1 018.53, 106.23-623.42, 3.53-16.47, 6 104.50-15 933.93, 1 047.58-1 750.15, 537.91-2 582.74, 332.83-581.28, 348.22-760.81, 350.65-2 189.73, 2.06-39.22 μg·g-1. Conclusion: The established method is accurate, stable and reproducible,and can provide a research method for the quality control of Shipi powder.
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