安全监测

党参中41种元素分析及风险评估*

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  • 1.中国食品药品检定研究院,北京 102629;
    2.重庆市食品药品检测研究院,重庆 401121;
    3.甘肃省药品检验研究院,兰州 730070;
    4.岛津企业管理(中国)有限公司,北京 100020
第一作者 左甜甜 Tel:(010)53851453;E-mail: zuotiantian2011@163.com
张 毅 Tel:(023)86072211;E-mail:zhangyi@cqifdc.org.cn
**马双成 Tel:(010)67095272;E-mail:masc@nifdc.org.cn;孙 磊 Tel:(010)67095891;E-mail:dasunlei@sina.com

收稿日期: 2021-08-20

  网络出版日期: 2024-06-24

基金资助

*国家十三五“重大新药创制”课题(2018ZX09735006); 中国食品药品检定研究院中青年基金资助项目(2020A3)

Analysis of 41 elements in Codonopsis pilosula(Franch.) Nannf. and risk assessment*

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  • 1. National Institutes for Food and Drug Control, Beijing 102629, China;
    2. Chongqing Institute for Food and Drug Control, Chongqing 401121, China;
    3. Gansu Institute for Drug Control, Lanzhou 730070, China;
    4. Shimadzu China Innovation Center, Shimadzu (China) Co., Ltd, Beijing 100020, China

Received date: 2021-08-20

  Online published: 2024-06-24

摘要

目的: 建立党参药材中41种元素含量测定方法,并对其中的毒性元素Pb、Cd、As、Cu、Al、Mn、Ni的风险进行评估,以期为党参的质量监测提供依据。方法: 样品经微波消解后,采用电感耦合等离子体质谱法(ICP-MS)进行分析,考虑药食同源品种的使用特点,对其中的Al、As、Cu、Pb、Mn、Ni、Cd的风险进行风险评估。结果: 各元素的线性关系良好;方法检测限为0.001~0.085 μg·kg-1;精密度良好;回收率在82.6%~117.4%;有证标准物质的测定结果与标示值相一致。党参中Al、Ba、Ca、Fe、Mg、P、K、Ga、Mn的含量较高(>50 mg·kg-1)。非致癌性风险评估结果表明,除了1批党参(药食两用)中As元素的危害指数(HI)高以外,其余元素的风险均可被接受。致癌性风险评估结果表明,无论作为中药还是食品使用,所有批次样品中Pb的致癌风险(CR)均低于1×10-4,风险可接受;As的评估结果表明,仅有1批党参药用时,其CR高于1×10-4;作为食品使用时,所有批次党参的CR均高于1×10-4,风险需要被关注。结论: 本研究所建立的多元素分析方法灵敏度高,专属性好,与风险评估技术相结合,可为党参的质量监测提供技术储备。

本文引用格式

左甜甜, 张毅, 马潇, 李晓东, 樊昌俊, 金红宇, 孙磊, 马双成 . 党参中41种元素分析及风险评估*[J]. 药物分析杂志, 2023 , 43(3) : 440 -447 . DOI: 10.16155/j.0254-1793.2023.03.10

Abstract

Objective: To establish the method for the determination of 41 elements in Codonopsis pilosula(Franch.)Nannf. and evaluate the risk of these toxic elements, including Pb, Cd, As, Cu, Al, Mn and Ni, in order to provide basis for the quality monitoring of Codonopsis pilosula. Methods: After microwave digestion, the samples were analyzed by inductively coupled plasma mass spectrometry (ICP-MS). Considering the use characteristics of medicine and food homologous varieties, the non-carcinogenic and carcinogenic risks of Al, As, Cu, Pb, Mn, Ni and Cd were assessed. Results: As a result, the linear relationships of the elements were good. The limits of detection were from 0.001 to 0.085 μg·kg-1. The precision was also good. The recoveries were between 82.6%-117.4%. The results of certified reference materials were consistent with the marked values. The contents of Al, Ba, Ca, Fe, Mg, P, K, Ga and Mn in Codonopsis pilosula(Franch.)Nannf. were larger than 50 mg·kg-1. The results of non-carcinogenic risk assessment showed that except for one batch of Codonopsis pilosula(Franch.)Nannf. whose HI of As was higher than 1 used as both medicine and food, the risks of other elements were acceptable. The results of carcinogenic risk assessment showed that the CRs of Pb in all batches were lower than 1×10-4, and the risk was acceptable. The risk assessment results of as showed that the CR of one batch of Codonopsis pilosula used as traditional Chinese medicine was higher than 1×10-4. When used as food, the CRs of all batches of Codonopsis pilosula were higher than 1×10-4, and the risk could not be neglected. Conclusion: The multi-element analysis method established in this study has high sensitivity and good specificity. The method established combined with risk assessment technology provides technical supports for the quality monitoring of Codonopsis pilosula(Franch.)Nannf.

参考文献

[1] 朱恩圆, 贺庆, 王峥涛, 等. 党参化学成分研究[J].中国药科大学学报,2001, 32(2):94
ZHU EY, HE Q, WANG ZT, et al. Chemical study on the root of Codonopsis pilosula[J]. J China Pharm Univ, 2001, 32(2):94
[2] 孙政华, 邵晶, 郭玫. 党参化学成分及药理作用研究进展[J].安徽农业科学, 2015, 43(33): 174
SUN ZH, SHAO J, GUO M. Research progress of Codonopsis pilosula chemical component and pharmacological effects[J].J Anhui Agric Sci, 2015, 43(33): 174
[3] 任风鸣, 谢贤明, 章文伟, 等. 不同产地川党参质量评价研究[J].实用中医药杂志, 2014, 30(3): 241
REN FM, XIE XM, ZHANG WW, et al. Study on quality evaluation of Codonopsis pilosula from different areas[J].J Pract Tradit Chin Med, 2014, 30(3): 241
[4] GAO JP. Transcriptome sequencing of Codonopsis pilosula and identification of candidate genes involved in polysaccharide biosynthesis[J].PLoS One, 2015, 10(2): 3833
[5] 李成义, 魏学明, 王明伟, 等. 硫熏对党参中党参炔苷含量的影响[J].中国现代中药, 2010(12): 11
LI CY, WEI XM, WANG MW, et al. Effect of sulphur fumigation on lobetyolin in Codonopsis Radix[J].Mod Chin Med, 2010(12): 11
[6] 郝桂明, 李欢欣, 赵春杰, 等. 反相高效液相色谱法测定党参中苍术内酯Ⅲ含量[J].沈阳药科大学学报, 2002, 19(5): 337
HAO GM, LI HX, ZHAO CJ, et al. Determination of the content of atractylenoide Ⅲ in the Codonopsis pilosula (Franch.)Nannf. by RP-HPLC[J].J Shenyang Pharm Univ, 2002, 19(5): 337
[7] 黄健, 车晓平, 陈志峰, 等. 不同产地党参中党参炔苷的含量测定[J].北京中医药, 2011, 30(6): 467
HUANG J, CHE XP, CHEN ZF, et al. Determination of Codonopsis pilosula alkynoside in Codonopsis pilosula from different habitats[J].Beijing J Tradi Chin Med, 2011, 30(6): 467
[8] 邹元锋, 陈兴福, 杨文钰, 等. 不同生长年限党参氨基酸组分分析及营养价值评价[J].食品与发酵工业, 2010, 36(6): 146
ZOU YF, CHEN XF, YANG WY, et al. Analysis of amino acids in different ages of Radix Codonopsis and their nutritional Evaluation[J].Food Ferm Indus, 2010, 36(6): 146
[9] DI BELLA G, POTORTI AG, LO TURCO V, et al. Trace elements in Thunnus thynnus from Mediterranean sea: benefit-risk assessment for consumers[J].Food Addit Contam Part B Surveill, 2015, 8(3):175
[10] JAISHANKAR M, TSETEN T, ANBALAGAN N, et al. Toxicity, mechanism and health effects of some heavy metals[J].Interdiscip Toxicol, 2014, 7: 60
[11] SIROT V, GUERIN T, VOLATIER JL, et al. Dietary exposure and biomarkers of arsenic in consumers of fish and shellfish from France[J].Sci Total Environ, 2009, 407(6):1875
[12] ZHOU J, LIANG J, HU YM, et al. Exposure risk of local residents to copper near the largest flash copper smelter in China[J].Sci Total Environ, 2018, 630: 453
[13] 于朝云, 杨慧. 微量元素与人体生理功能的关系[J].山东医药, 2009, 49(9):113
YU CY, YANG H. Relationship between trace elements and human physiological function[J].Shandong Med J, 2009, 49(9):113
[14] KHAN S, CAO Q, ZHENG YM, et al. Health risks of heavy metals in contaminated soil and food crops irrigated with wastewater in Beijing, China [J].Environ Pollut, 2008, 152(3):686
[15] MAHMOOD A, MALIK RN. Human health risk assessment of heavy metals via consumption of contaminated vegetables collected from different irrigation sources in Lahore [J].Arab J Chem, 2013, 7(1):91
[16] SUN L, MA X, JIN HY, et al. Geographical origin differentiation of Chinese Angelica by specific metal element fingerprinting and risk assessment[J].Environ Sci Pollut Res Int, 2020, 27: 45018
[17] ZUO TT, JIN HY, ZHANG L, et al. Innovative health risk assessment of heavy metals in Chinese herbal medicines based on extensive data[J].Environ Sci Pollut R, 2020, 159: 104987
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