宜宾黄柏和四川其他产区黄柏一测多评和指纹图谱对比研究<sup>*</sup>

何兵, 吴建明, 梁思成, 田吉, 杨世艳

doi:10.16155/j.0254-1793.2023.03.17

药物分析杂志 >

2023 , Vol. 43 >Issue 3: 494 - 508

DOI: https://doi.org/10.16155/j.0254-1793.2023.03.17

标准研讨

宜宾黄柏和四川其他产区黄柏一测多评和指纹图谱对比研究^*

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1.西南医科大学公共实验技术中心,泸州 646000;
2.西南医科大学基础医学院,泸州 646000;
3.西南医科大学附属医院,泸州 646000;
4.西南医科大学药学院,泸州 646000;
5.西南医科大学附属中医医院,泸州 646000

第一作者 Tel:18982785351;E-mail:lyhb2008@126.com

**Tel:13982770721;E-mail:lyysy2010@126.com

修回日期: 2023-01-04

网络出版日期: 2024-06-24

基金资助

*宜宾县人民政府-西南医科大学专项项目(2017-YBXNYD-3);四川省教育厅重点项目(No. 15ZA0165);泸州市科技局-泸州医学院联合基金(No. 2013LZLY-K61)

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Comparative study on QAMS and fingerprint of Phellodendron chinense Schneid. from Yibin and other Sichuan producing areas^*

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1. Public Center of Experimental Technology, Southwest Medical University, Luzhou 646000, China;
2. School of Basic Medical Sciences, Southwest Medical University, Luzhou 646000, China;
3. Affiliated Hospital of Southwest Medical University, Luzhou 646000, China;
4. School of Pharmacy,Southwest Medical University, Luzhou 646000, China;
5. Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou 646000, China

Revised date: 2023-01-04

Online published: 2024-06-24

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摘要

目的: 对比研究宜宾黄柏和四川其他产区黄柏多指标成分含量和指纹图谱,以评价其品质差异。方法: 以盐酸小檗碱为参照物,建立宜宾黄柏和四川其他产区黄柏HPLC指纹图谱,同时分别以斜率校正、多点校正和单点校正建立绿原酸、盐酸黄柏碱、木兰花碱、5-O-阿魏酰奎宁酸、盐酸药根碱、盐酸巴马汀相对于盐酸小檗碱的相对校正因子,并以此计算各个成分的含量,实现一测多评。同时采用外标法测定上述7个成分的含量,并对比外标法与采用不同校正方式的一测多评之间差异,以验证一测多评法的准确性和可行性。结果: 建立了宜宾黄柏和四川其他产区黄柏HPLC指纹图谱,标定了10个共有峰,指认了其中7个共有峰,21批宜宾黄柏和17批四川其他产区黄柏的相似度分别在0.987~1.000和0.986~1.000。21批宜宾黄柏和17批四川其他产区黄柏中7个成分的含量计算值与实测值间均无显著差异。结论: 采用多指标定量指纹图谱评价黄柏的质量是准确可行的,无论宜宾黄柏还是四川其他产区黄柏,其批间差异均较小,相似度较高。宜宾产的精品去皮黄柏,有效成分含量处于黄柏中较高水平。

关键词： 一测多评; 指纹图谱; 黄柏; 绿原酸; 盐酸黄柏碱; 木兰花碱; 5-O-阿魏酰奎宁酸; 盐酸小檗碱

本文引用格式

何兵, 吴建明, 梁思成, 田吉, 杨世艳 . 宜宾黄柏和四川其他产区黄柏一测多评和指纹图谱对比研究^*[J]. 药物分析杂志, 2023 , 43(3) : 494 -508 . DOI: 10.16155/j.0254-1793.2023.03.17

Abstract

Objective: To comparative study on the content of multiple index components and fingerprint of Phellodendron chinense Schneid. from Yibin and other producing areas in Sichuan, in order to evaluate their quality differences. Methods: The HPLC fingerprints of Phellodendron chinense Schneid. from Yibin and other producing areas were established with berberine hydrochloride as reference substance. At the same time, the relative correction factors(RCFs) of chlorogenic acid, phellodendrine hydrochloride, magnoflorine, 5-O-feruloylquinic acid, jatrorrhizine hydrochloride and palmatine hydrochloride relative to berberine hydrochloride were established by slope correction(SC), multi-point correction(MPC) and single point correction(SPC), respectively. The content of each component was calculated by this method to realize quantitative analysis of multi-components by single marker (QAMS). At the same time, the contents of these seven components were determined by external standard method(ESM), and the difference between the external standard method and the QAMS with different correction methods compared to verify the accuracy and feasibility of the QAMS. Results: The fingerprint of Phellodendron chinense Schneid. from Yibin and other producing areas in Sichuan was established. All tested samples contained the 10 common peaks, 7 of which were verified, the similarity of 21 batches of Phellodendron chinense Schneid. from Yibin and other producing areas was in the range of 0.987-1.000 and 0.986-1.000, and there were no significant differences between the quantitative results of 7 ingredients of QAMS and external standard method in 21 batches of Phellodendron chinense Schneid. from Yibin and 17 batches of other producing areas. Conclusion: It is accurate and feasible to evaluate the quality of Phellodendron chinense Schneid. by multi-marker components quantified fingerprint. Whether Phellodendron chinense Schneid. from Yibin or other producing areas, the difference between batches is small and the similarity is high. Eliminating cortex Phellodendron chinense Schneid. in Yibin, the content of effective components is at a high level in Phellodendron chinense Schneid.

Key words： QAMS; fingerprint; Phellodendron chinense Schneid.; chlorogenic acid; phellodendrine hydrochloride; magnoflorine; 5-O-feruloylquinic acid; berberine hydrochloride

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