Objective: To establish an UPLC method for simultaneous determination of 5-hydroxymethyl furfuraldehyde (5-HMF), cimicifugoside, calycosin-7-glucoside, cimicifugin, 4’-O-beta-glucopyranosyl-5-O-methylvisamminol, calycosin, sec-O-glucosylhamaudol, 6-gingerol, schisandrin and fraxinellone in Guben Kangmin decoction, so as to analyze the variation of the 10 ingredients after compatibility. Methods: The samples were separated on an ACQUITY UPLC BEH C18 column (2.1 mm×100 mm, 1.7 μm) by gradient elution with acetonitrile-0.1% formic acid aqueous solution at a flow rate of 0.25 mL·min-1. The detection wavelength was 254 nm. The column temperature was controlled at 30 ℃. Results: 5-HMF,cimicifugoside, calycosin-7-glucoside, cimicifugin, 4’-O-beta-glucopyranosyl-5-O-methyl-visamminol, calycosin, sec-O-glucosylhamaudol, 6-gingerol, schisandrin and fraxinellone exhibited good linearity(r2=0.999 5-1.000)among the ranges of 156.00-10000, 12.50-1600, 3.12-400, 12.50-1600, 6.25-800, 0.78-100, 3.90-500, 20.50-1600, 0.58-75 and 12.50-200 ng, respectively. The average recoveries (RSD<3%, n=6) were between 98.3%-102.3%. After the compatibility, the contents of 6-gingerol, calycosin-7-glucoside, 5-HMF, schisandrin and fraxinellone increased significantly (P<0.05 or P<0.01), while there was no significant change in the contents of cimicifugoside, cimicifugin, 4’-O-beta-glucopyranosyl-5-O-methyl-visamminol, calycosin and sec-O-glucosylhamaudol. Conclusion: The method can be used for the quality control of Guben Kangmin decoction and its preparations, and can provide scientific basis for the clinical compatibility and application of Guben Kangmin decoction.
[1] 卢传坚,郭洁. 基于发病特点探讨慢性荨麻疹的治疗法则[J].中医杂志,2017,58(15):1296
LU CJ, GUO J. Discussing treatment principle of chronic urticaria based on its occurrence regularity[J].J Tradit Chin Med, 2017, 58(15):1296
[2] 沈萃萃. 固本抗敏方治疗慢性自发性荨麻疹的临床疗效观察及机制初探[D].广州: 广州中医药大学, 2018
SHEN CC. Clinical Observation and Mechanism Exploration of Guben Kangmin Decoction on Chronic Spontaneous Urticaria[D].Guangzhou:Guangzhou University of Chinese Medicine, 2018
[3] 王维皓,李娟,高慧敏,等. 从HPLC特征图谱分析姜在炮制过程中的化学成分变化[J].药物分析杂志,2009,29(8):1248
WANG WH, LI J, GAO HM, et al. Chemical ingredients change of ginger processing with HPLC specific chromatogram[J].Chin J Pharm Anal, 2009, 29(8):1248
[4] GUO Z, LOU Y, KONG M, et al. A systematic review of phytochemistry, pharmacology and pharmacokinetics on Astragali Radix: implications for Astragali Radix as a personalized medicine[J].Int J Mol Sci, 2019, 20(6):14636
[5] 马天成,孙宇,张金玲,等. UPLC-MS同时测定黄芪中10种化合物含量[J].中药材,2019,42(4):838
MA TC, SUN Y, ZHANG JL, et al. Simultaneous determination of ten components in Guben Kangmin Decoction by UPLC-MS Astragali Radix[J].J Chin Med Mater, 2019, 42(4):838
[6] 吴拾保,邓渝,袁铭铭,等. 玉屏风口服液1个异黄酮类及3个色原酮类成分的含量测定[J].药物分析杂志,2016,36(12):2221
WU SB, DENG Y, YUAN MM, et al. Simultaneous determination of one isoflavonoids and three chromones in Yupingfeng oral liquid by HPLC[J].Chin J Pharm Anal, 2016, 36(12):2221
[7] WANG L, ZHAO MB, JIANG Yong, et al. Quality standard study on Dictamni Cortex [J].J Chin Pharm Sci, 2017, 26(4):298
[8] 刘移兰,蔡卫华,窦志华,等. 一测多评法同时测定五味子中12个木脂素类成分的含量[J].药物分析杂志,2019,39(7):1207
LIU YL, CAI WH, DOU ZH, et al. Simultaneous determination of 12 lignans in Schisandrae Chinensis Fructus by quantitative analysis of multi-components with single marker[J].Chin J Pharm Anal, 2019, 39(7):1207
[9] 张奇,叶正良,李德坤,等. 不同加工方法和成熟阶段对五味子主要化学成分的影响[J].中国中药杂志,2011,36(13):1722
ZHANG Q, YE ZL, LI DK, et al. Influence of different processing method and mature stage on main chemical constituents of Schisandra chinensis[J].China J Chin Mater Med, 2011, 36(13):1722
[10] VIJAYAKUMAR K, RAMANATHAN T. Musa acuminata and its bioactive metabolite 5-hydroxymethylfurfural mitigates quorum sensing (LAS and RHL) mediated biofilm and virulence production of nosocomial pathogen Pseudomonas aeruginosa in vitro[J].J Ethnopharmacol, 2019, 246:112242
[11] 李恩灿,贺玖明,靳洪涛,等. 5-羟甲基糠醛的药理和毒理研究进展[J].中国药物警戒,2018,15(4):210
LI EC, HE JM, JIN HT, et al. Research progress of pharmacological activity and toxicity of 5-hydroxymethylfurfural[J].Chin J Pharmacov, 2018, 15(4):210
[12] YAMADA P, NEMOTO M, SHIGEMORI H, et al. Isolation of 5-(hydroxymethyl)furfural from Lycium chinense and its inhibitory effect on the chemical mediator release by basophilic cells[J].Planta Med, 2011, 77(5):434
[13] BAUER-MARINOVIC M, TAUGNER F, FLORIAN S, et al. Toxicity studies with 5-hydroxymethylfurfural and its metabolite 5-sulphooxymethylfurfural in wild-type mice and transgenic mice expressing human sulphotransferases 1A1 and 1A2[J].Arch Toxicol, 2012, 86(5):701
[14] SACHSE A, MEINL W, SOMMER Y, et al. Bioactivation of food genotoxicants 5-hydroxymethylfurfural and furfuryl alcohol by sulfotransferases from human, mouse and rat: a comparative study[J].Arch Toxicol, 2016, 90:137
[15] 薛雪,王浩,贾天颖,等. 不同生长方式及年限防风饮片质量的比较研究及对《中国药典》防风饮片标准的思考[J].中国中药杂志,2019,44(18):4034
XUE X, WANG H, JIA TY, et al. Comparative study on quality of decoction pieces of Saposhnikovia divaricata with different growth patterns and years and thinking of standard of decoction pieces of S. divaricata in Chinese Pharmacopoeia[J].China J Chin Mater Med, 2019, 44(18):4034
[16] TAI J, CHEUNG SS. Anti-proliferative and antioxidant activities of Saposhnikovia divaricata[J].Oncol Rep, 2017,18(1):227
[17] 刘双利,姜程曦,赵岩,等. 防风化学成分及其药理作用研究进展[J].中草药,2017,48(10):2146
LIU SL, JIANG CX, ZHAO Y, et al. Advance in study on chemical constituents of Saposhnikovia divaricata and their pharmacological effects[J].Chin Tradit Herb Drugs, 2017, 48(10):2146
[18] 张冬青,王海宝,王淑芳,等. 毛蕊异黄酮生物活性的研究进展[J].中国中药杂志,2015,40(22):4339
ZHANG DQ, WANG HB, WANG SF, et al. Research achievements on biological activities of calycosin[J].China J Chin Mater Med, 2015, 40(22):4339
[19] 韦秋雨. 生姜中活性成分6-姜酚的纳米制剂研究[D].镇江:江苏大学,2019
WEI QY. Study on the Nano-Formulations of 6-Gingerol, the Main Active Constitute of Ginger[D].Zhengjiang:Jiangsu University, 2019
[20] 万鹏,赵瑞芝,胡巧红. 中药多糖对黄芩苷的溶解度及其药动学的影响[J].广东药科大学学报, 2017, 33(4):439
WAN P, ZHAO RZ, HU QH. Effects of polysaccharides on the solubility and pharmacokinetics of baicalin[J].J Guangdong Pharm Univ, 2017, 33(4):439
[21] ZHAO Y, WAN P, WANG J, et al. Polysaccharide from vinegar baked radix bupleuri as efficient solubilizer for water-insoluble drugs of Chinese medicine[J].Carbohydr Polym, 2020, 229:115473
[22] LIU F, SUN L, YOU G, et al. Effects of astragalus polysaccharide on the solubility and stability of 15 flavonoids[J].Int J Biol Macromol, 2020, 143:873
[23] 曾灵娜,王伽伯,张萍,等. 中药调剂规范化研究(Ⅲ):含不同类型成分的中药对大黄蒽醌煎出量的影响[J].中国中药杂志,2012,37(2):202
ZENG LN, WANG JB, ZHANG P, et al. Research on standardized preparation of traditional Chinese medicine (Ⅲ):difference of extracting quantity of anthraquinones from mixed decoction of rhubarb with TCMs containing different ingredients[J].China J Chin Mater Med,2012,37(2):202
[24] 赵奎君,李卓明,陈小莺,等. 从当归补血汤的研究探讨和分析中药复方共煎的合理性[J].世界科学技术-中医药现代化,2009,11(2):294
ZHAO KJ, LI ZM, CHEN XY, et al. The rationality of mixed preparation of traditional herbal decoction: from the perspective of Danggui Buxue Tang[J].World Sci Technol-Mod Tradit Chin Med, 2009, 11(2):294
