成分分析

反相离子对色谱法测定四氢姜黄素的含量*

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  • 1.成都中医药大学药学院,成都 611137;
    2.四川省中医药科学院,成都 610041
第一作者 Tel:13551049713;E-mail:zhuning028@foxmail.com
** 凌海燕 Tel:18919561365;E-mail:hyling1012@foxmail.com
李小芳 Tel:13808195110;E-mail:lixiaofang918@163.com

收稿日期: 2021-09-16

  网络出版日期: 2024-06-24

基金资助

* 科技部重大新药创制专项资助项目(2018ZX09731-008)

Ion-pairing RP-HPLC method to determine tetrahydrocurcumin*

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  • 1. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;
    2. Sichuan Academy of Chinese Medicine Science, Chengdu 610041, China

Received date: 2021-09-16

  Online published: 2024-06-24

摘要

目的: 建立测定四氢姜黄素含量的离子对反相高效液相色谱法。方法: 采用Agilent ZORBAX Extend-C18(150 mm×4.6 mm,5.0 μm)色谱柱,以乙腈-0.05%氨水(含10 mmol·L-1十二烷基三甲基氯化铵)(38∶62)为流动相进行等度洗脱,检测波长288 nm,柱温25 ℃。结果: 四氢姜黄素进样量在0.149~7.452 μg范围内与峰面积线性关系良好(r=0.999 9),平均回收率为99.9%(RSD=0.68%);检测限和定量限分别为0.011 μg和0.044 μg,且四氢姜黄素与杂质能够完全分离。按本法测得3批原料药含量分别为98.6%、98.3%和98.3%。结论: 本含量测定方法简单、快速,专属性好,能够满足四氢姜黄素原料药含量测定的要求。

本文引用格式

朱宁, 凌海燕, 杨安东, 赵军宁, 李小芳 . 反相离子对色谱法测定四氢姜黄素的含量*[J]. 药物分析杂志, 2022 , 42(5) : 773 -779 . DOI: 10.16155/j.0254-1793.2022.05.04

Abstract

Objective: To establish an ion-pairing reverse phase HPLC determination method for detecting the concentration of tetrahydrocurcumin in active pharmaceutical ingredient(API). Methods: Analysis was carried out with an Agilent ZORBAX Extend-C18(150 mm×4.6 mm,5.0 μm)column; the mobile phase was composed of acetonitrile-0.05% amnonia and 10 mmol·L-1 dodecyl trimethyl ammonium chloride in water(38∶62), with linear gradient elution at a flow rate of 1 mL·min-1. The detection wavelength was 288 nm. The column temperature was 25 ℃. Results: The linear range of the calibration curve for tetrahydrocurcumin was 0.149-7.452 μg(r=0.999 9). The average recovery was 99.9%(RSD=0.68%).Limits of quantity and detection were 0.011 μg and 0.044 μg, respectively. Tetrahydrocurcumin and its related substances were completely separated. According to this method, the contents of three batches of API were 98.6%, 98.3% and 98.3%, respectively. Conclusion: Because of its simplicity, accuracy and specificity, this method is suitable for the determination of content of tetrahydrocurcumin API.

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