质量分析

干蟾皮及其炮制品的指纹图谱建立与化学模式识别*

展开
  • 南通市食品药品监督检验中心,南通 226006
第一作者 Tel:(0513)83548660;E-mail:656074350@qq.com

收稿日期: 2021-05-18

  网络出版日期: 2024-06-24

基金资助

* 江苏省南通市科技计划项目(JC2019134)

Establishment of fingerprint and identification of chemical pattern of Bufonis Coriumand processed products*

Expand
  • Nantong Food and Drug Control Center, Nantong 226006, China

Received date: 2021-05-18

  Online published: 2024-06-24

摘要

目的: 建立干蟾皮生品及炮制品UPLC指纹图谱,并同时测定干蟾皮中指标性成分日蟾毒它灵、沙蟾毒精、蟾蜍它里定、蟾毒它灵、华蟾毒它灵、蟾毒灵、华蟾酥毒基、脂蟾毒配基、沙蟾毒精-3-辛二醇辛酯的含量,通过化学模式识别控制干蟾皮质量。方法: 干蟾皮的甲醇提取液的分析采用Waters Acquity UPLC BEH色谱柱(100 mm×2.1 mm,1.7 μm),以乙腈-0.5%乙酸为流动相,梯度洗脱,流速为0.2 mL·min-1,柱温为30 ℃,检测波长为296 nm。结果: 12批生品和12批炮制品的总含量分别为3.18~4.69 mg·g-1和0.91~2.77 mg·g-1,指纹图谱中均有9个共有峰,相似度分别为0.963~0.991和0.692~0.988,主成分分析和聚类分析能很好地识别干蟾皮的生品和炮制品。结论: 该方法稳定、可靠,可为干蟾皮的质量控制、炮制工艺,进一步开发研究提供科学依据。

本文引用格式

周谧, 徐丹洋, 孙成静, 钱洁 . 干蟾皮及其炮制品的指纹图谱建立与化学模式识别*[J]. 药物分析杂志, 2022 , 42(5) : 875 -883 . DOI: 10.16155/j.0254-1793.2022.05.17

Abstract

Objective: To control the quality of Bufonis Corium with chemical pattern, UPLC fingerprints of raw materials and processed products of Bufonis Corium were establish and the contents of the index components of Bufonis Corium, such as gamafotalin, arenobufagin, bufotalidin, bufotaline, cinobufotalin, bufalin, cinobufagin, resibufogenin and 3-arenobufagyl-subericacid were determined. Methods: The methanol extract of dry ecdysis was analyzed by Waters Acquity UPLC BEH chromatographic column (100 mm×2.1 mm, 1.7 μm) with a mobile phase of acetonitrile -0.5% acetic acid aqueous solution in gradient elution.The flow rate was 0.2 mL·min-1, the column temperature was 30 ℃ and the detection was set at 296 nm. Results: The total contents of 12 batches of raw products and 12 batches of processed products were 3.18-4.69 mg·g-1 and 0.91-2.77 mg·g-1, there were 9 common peaks in the fingerprints, and the similarity were 0.963-0.991 and 0.692-0.988, respectively. The principal component analysis and cluster analysis could identify the raw and processed products of Bufonis Corium. Conclusion: The method is stable and reliable, and can provide scientific basis for quality control, processing technology and further development of Bufonis Corium.

参考文献

[1] 中科院中国动物志编辑委员会. 中国动物志两栖纲(下卷)[M]. 北京:科学出版社, 2009:529
Editorial Committee of Chinese Zoology, Chinese Academy of Sciences. Chinese Zoology Amphibians (Volume 2)[M]. Beijing:Science Press, 2009:529
[2] LI FJ, HU JH, REN X, et al. Toad venom:a comprehensive review of chemical constituents, anticancer activities, and mechanisms[J].Arch Pharm(Weinheim), 2021, 354(7):e2100060
[3] 刘晓兰, 吴南珍, 徐鹏飞. 华蟾素联合痰热清注射液治疗慢性乙型肝炎39例临床疗效观察[J].中国实用医药, 2009, 4(32):117
LIU XL, WU NZ, XU PF. Clinical observation on the treatment of 39 cases of chronic hepatitis B with Huachansu and Tanreqing injection[J].China Pract Med, 2009, 4(32):117
[4] 张英, 邱鹰昆, 刘珂.中华大蟾蜍的研究进展[J].中草药, 2006, 37(12):1905
ZHANG Y, QIU YK, LIU K. Advances in studies on Bufobufo gargarizans[J].Chin Tradit Herb Drugs, 2006, 37(12):1905
[5] BAI Y, WANG XY, CAI MS, et al. Cinobufagin suppresses colorectal cancer growth via STAT3 pathway inhibition[J].Am J Cancer Res, 2021, 11(1):200
[6] 周谧, 傅兴圣, 朱琳, 等.干蟾皮药材质量标准研究[J].药学与临床研究, 2017, 25(3):209
ZHOU M, FU XS, ZHU L, et al. Study on quality standards of Bufobufo gargarizans[J].Pharm Clin Res, 2017, 25(3):209
[7] 张宁,汪晨,顾伟,等. 蟾毒灵对人肝癌细胞HepG2细胞周期的影响及其机制研究[J].现代肿瘤医学, 2010,18(3):439
ZHANG N, WANG C, GU W, et al. The effect and related mechanism of bufalin on the cell cycle of HepG2 cell[J].J Mod Oncol, 2010,18(3):439
[8] 方凡夫, 张亚妮, 顾伟, 等. 蟾毒灵抑制人白血病多药耐药K562/VCR细胞株增殖的实验研究[J].中药新药与临床药理, 2010,21(3):213
FANG FF, ZHANG YN, GU W, et al. Experimental study on bufalin in hibiting the proliferation of multidrug-resistance human leukaemia cell line K562/VCR[J].Tradit Chin Drug Res Clin Pharm,2010, 21(3):213
[9] NAKATA M, KAWAGUCHI S, OIKAWA A, et al. An aqueous extract from toad skin prevents gelatinase activities derived from fetal serum albumin and serum-free culture medium of human breast carcinoma MDA-MB-231 cells[J].Drug Discov Ther, 2015, 9(6):417
[10] 应金琴, 杨明, 张普照, 等. 蟾酥的炮制历史沿革、化学成分及药理活性研究进展[J].中国中药杂志, 2021, 46(14):3529
YING JQ, YANG M, ZHANG PZ, et al. Processing evolution, chemical components and pharmacological effects of Bufonis Venenum[J].Chin J Chin Mater Med, 2021, 46(14):3529
[11] 赵大洲, 陈继永, 秦勇, 等. 中华大蟾蜍蟾酥和蟾皮化学成分的比较分析[J].天津药学, 2006, 18(4):21
ZHAO DZ, CHEN JY, QIN Y, et al. A comparison study on the chemical constituents of Venenum Bufonis and the skin of toad[J].Tianjin Pharm, 2006, 18(4):21
[12] 喻昌燕, 孟令杰, 姜念, 等. 蟾皮化学成分和药理活性的研究进展[J].中草药, 2021, 52(4):1206
YU CY, MENG LJ, JIANG N, et al.Research progress on chemical constituents and pharmacological activities of Bofonis Corium[J].Chin Tradit Herb Drugs, 2021, 52(4):1206
[13] 刘旭, 王彧. 蟾皮水提物及其多肽抗乳腺癌作用及机制的初步研究[J].中国药理学与毒理学杂志, 2019, 33(9):712
LIU X, WANG Y.Preliminary study on the anti-breast cancer effect and mechanism of the water extract of toad skin and its peptides[J].Chin J Pharm Toxicol, 2019, 33(9):712
[14] 张瑜. 蟾皮中吲哚烷胺类生物碱化学成分及抗炎活性机制研究[D]. 北京:中国中医科学院, 2020
ZHANG Y. The Chemical and Anti-inflammatory Activity Mechanis Research of Indolealkylamines in Toad Skin[D]. Beijing:China Academy of Chinese Medical Sciences, 2020
[15] 张磊, 蔡晓瑶, 张莉, 等. 蟾皮提取物固体脂质纳米粒的冻干工艺研究[J].安徽医药, 2020, 24(1):78
ZHANG L, CAI XY, ZHANG L, et al. Study on the freezedrying technology of solid lipid nanoparticles from toad skin extract[J].Anhui Med Pharm J, 2020, 24(1):78
[16] 韩婷, 李艳芳, 刘金旗, 等. 中华大蟾皮化学成分研究[J].现代中药研究与实践, 2019, 33(5):16
HAN T, LI YF, LIU JQ, et al. Chemical constituents from the skin of Bufobufo gargarizans Cantor[J].Res Pract Chin Med, 2019, 33(5):16
[17] ZHAO JM, ZHANG QS, ZOU GY, et al. Arenobufagin, isolated from toad venom, inhibited epithelial-to-mesenchymal transition and suppressed migration and invasion of lung cancer cells via targeting IKK beta/NF kappa B signal cascade[J].J Ethnopharmacol, 2020, 250:112492
[18] 薛鹏, 李林潞, 徐芃芃, 等. 干蟾皮在治疗小细胞肺癌中的应用[J].中华中医药学刊, 2019, 37(6):1356
XUE P, LI LL, XU PP, et al. Application of dry ecdysis in treatment of small cell lung cancer[J].Chin Arch Tradit Chin Med, 2019, 37(6):1356
[19] NIU JJ, WANG JM, ZHANG Q, et al. Cinobufagin-induced DNA damage response activates G2/M checkpoint and apoptosis to cause selective cytotoxicity in cancer cells[J].Cancer Cell Int, 2021, 21(1):446
[20] 姜珊, 谢明, 郑佳, 等. 蟾蜍类药材本草考证[J].亚太传统医药, 2020, 16(12):95
JIANG S, XIE M, ZHENG J, et al. Textual research ontoad medicinal materials [J]. Asia Pac Tradit Med, 2020, 16(12):95
[21] WU JH, CAO YT, PAN HY, et al. Identification of antitumor constituents in Toad Venom by spectrum-effect relationship analysis and investigation on its pharmacologic mechanism[J].Molecules, 2020, 25(18):4269
文章导航

/