目的:研究正常大鼠和伪无菌大鼠口服雄黄后,砷在全血中的药代动力学行为及其在尿液和粪便中排泄过程的差异,探讨肠道菌群对雄黄中砷体内行为的影响。方法:大鼠口服硫酸新霉素、硫酸链霉素、杆菌肽抗生素混合物,构建伪无菌大鼠模型。正常大鼠和伪无菌大鼠分别给予雄黄后,于不同时间点收集血液、尿液和粪便,采用氢化物发生-原子荧光光谱技术(HG-AFS)测定总砷含量。砷灯电流70 mA,负高压250 V,载气流量400 mL·min-1,屏蔽气流量1 000 mL·min-1。结果:正常大鼠与伪无菌大鼠全血中砷的Tmax分别为48.00和89.14 h,0~8 h内尿液中砷的排泄量分别为76.36和41.24 μg。与正常大鼠相比,伪无菌大鼠全血中砷的Tmax显著增加,0~8 h内尿液中砷的排泄量显著降低。结论:肠道菌群加快了砷的吸收,但对总砷浓度无明显影响,并在给药后8 h促进了尿砷的排泄。
Objective: To compare the blood pharmacokinetic behavior and urinary and fecal excretion of arsenic in normal and pseudo-sterile rats after oral administration of Realgar to clarify the influence of gut microbiota on the behavior of arsenic from Realgar in vivo. Methods: Rats were divided into normal and pseudo-sterile groups, and the pseudo-sterile rat model was established by oral gavaging of antibiotic mixtures of neomycin sulfate, streptomycin sulfate, and bacitracin.The blood, urine and feces were collected at different time points or periods after oral gavage of Realgar in normal and pseudo-sterile rats, respectively. Total arsenic content was determined by hydride generation atomic fluorescence spectroscopy(HG-AFS). The lamp current and negative pressure of arsenic were 70 mA and 250 V, respectively. The carrier and gasshield flow rate were 400 and 1 000 mL·min-1, respectively. Results: The Tmax of blood arsenic in normal and pseudo-sterile rats were 48.00 and 89.14 h, respectively. The arsenic urinary excretion in normal and pseudo-sterile rats were 76.34 and 41.24 μg, respectively. Compared with the normal rats, the Tmax of blood arsenic in pseudo-sterile rats was significantly increased, and arsenic urinary excretion was significantly reduced within 0-8 h. Conclusion: Gut microbiota accelerates the absorption of arsenic, but has no significant effect on total arsenic concentration, and promotes arsenic urinary excretion within 8 h after administration.
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