民族药研究专栏

基质辅助激光解吸质谱成像可视化分析巴戟天炮制品中化学成分的空间分布

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  • 1.中国食品药品检定研究院,北京 102629;
    2.岛津中国质谱中心,北京 100020
第一作者 Tel:(010)53852067;E-mail:qiaofei@nifdc.org.cn
* 马双成 Tel:(010)53852076;E-mail:masc@nifdc.org.cn
郑健 Tel:(010)53852080;E-mail:zhengjian@nifdc.org.cn

收稿日期: 2022-01-20

  网络出版日期: 2024-06-24

Visualization analysis of spatial distribution of chemical compositions in Morindae Officinalis Radix processed product by matrix-assisted laser desorption ionization mass spectrometry imaging

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  • 1. National Institutes for Food and Drug Control, Beijing 102629, China;
    2. Shimadzu China Innovation Center, Beijing 100020, China

Received date: 2022-01-20

  Online published: 2024-06-24

摘要

目的:采用质谱成像显微镜可视化分析巴戟天炮制品中环烯醚萜和寡糖的空间分布情况。方法:使用成像质谱显微镜对巴戟天不同炮制品进行空间分布数据采集,通过UPLC测定巴戟天不同炮制品中环烯醚萜的含量,判断不同炮制方法对环烯醚萜的影响,并验证质谱成像显微镜结果的准确性。结果:质谱成像显微镜可以实现环烯醚萜和寡糖的空间分布的可视化分析,结果与UPLC法含量测定结果基本一致。结论:质谱成像显微镜可运用于巴戟天炮制过程质量研究,为中药炮制研究提供一种新思路。

本文引用格式

乔菲, 戴胜云, 连超杰, 刘杰, 董静, 郑健, 马双成 . 基质辅助激光解吸质谱成像可视化分析巴戟天炮制品中化学成分的空间分布[J]. 药物分析杂志, 2022 , 42(8) : 1312 -1318 . DOI: 10.16155/j.0254-1793.2022.08.03

Abstract

Objective: To visual analyze the changes of iridoid and saccharous in Morindae Officinalis Radix processed product by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI). Methods: The data of spatial distribution of iridoid and saccharous in Morindae Officinalis Radix during different processing were acquired by MALDI-MSI. The contents of iridoid in the samples were determined by UPLC. The accuracy of MS imaging microscope was verified by the results of content determination. Results: The MS imaging microscope could distinguish different processed samples and realize the visualization analysis of spatial distribution of iridoid and saccharous in Morindae Officinalis Radix. The results were basically consistent with those determined by UPLC. Conclusion: MALDI-MSI can be used to study the processing quality of Morindae Officinalis Radix and this method provides new ideas for the Chinese medicine processing.

参考文献

[1] 中华人民共和国药典2020年版.一部[S].2020: 63
ChP 2020.Vol Ⅰ[S].2020: 63
[2] 乔智胜,苏中武,李承祜.巴戟天应用的名实沿革考[J].广西植物, 1993, 13(3):252
QIAO ZS, SU ZW, LI CH. Studies on the original plants of the traditional Chinese drug Bajitian recorded in the herbals[J].Guihaia,1993, 13(3):252
[3] 候宽昭.一种国药巴戟天的描述[J].植物分类学报, 1958, 7(4):325
HOU KZ. Description of a Chinese medical plant “Pa Chit Tien”[J].Acta Phytotaxonom Sin, 1958, 7(4): 325
[4] LUAN ZL, QIAO F, ZHAO WY, et al. Discovery of new iridoids as farnesoid X receptor agonists from Morinda officinalis: agonistic potentials and molecular[J].Chin J Chem, 2021, 39(5):1288
[5] 王玉磊. 巴戟天中主要环烯醚萜苷的研究[D].北京:北京中医药大学, 2011
WANG YL. Studies on Iridiod Glucosides in Radix Morindae Officinalis[D].Beijing:Beijing University of Chinese Medicine, 2011
[6] ZHANG HL, LI J, XIA JM, et al. Antioxidant activity and physicochemical properties of an acidic polysaccharide from Morinda officinalis[J].Int J Biol Macromol, 2013, 58:7
[7] 郝庆秀,康利平, 朱寿东, 等. 基于UPLC-Q-TOF-MSE技术的巴戟天低聚糖成分的快速鉴别研究[J].中国中药杂志, 2018, 43(6):1201
HAO QX, KANG LP, ZHU SD, et al. Rapidly identify oligosaccharides in Morinda officinalis by UPLC-Q-TOF-MSE[J].China J Chin Mater Med, 2018, 43(6):1201
[8] 李赛. 巴戟天根皮与木心化学成分的比较[J].中药通报, 1988(2):17
LI S. The differences of chemical constituents between cortex and wood-centre of Morinda officinalis[J].Bull Chin Mater Med, 1988(2):17
[9] 陈娥,周灿,廖莎,等.不同炮制去心法对巴戟天游离蒽醌含量的影响[J].中国药师, 2015, 18(11): 1985
CHEN E, ZHOU C, LIAO S, et al. Influence of different processing methods for removing woody core on free anthraquinones content in Morinda officinalis[J].China Pharm, 2015,18(11): 1985
[10] 徐吉银, 梁英娇, 丁平. 炮制方法对巴戟天有效成分水晶兰苷含量的影响[J].中药材, 2007, 30(1):20
XU JY, LIANG YJ, DING P. Determinnation of monotropein in Radix Morindae from different processed products by HPLC[J].J Chin Med Mater, 2007, 30(1):20
[11] 周灿,杨梓懿,许李,等. 不同炮制去心法对巴戟天多糖含量的影响(I)[J].湖南中医药大学学报, 2011,31(1):49
ZHOU C, YANG ZY, XU L, et al. Influence of different process to remove woody core on polysaccharide content of Morinda officinalis[J].J TCM Univ Hunan, 2011, 31(1):49
[12] 陈娥,周灿,廖莎, 等. 不同炮制去心法对巴戟天耐斯糖含量的影响[J].湖南中医药大学学报, 2016, 36(4): 31
CHEN E, ZHOU C, LIAO S, et al. Infuence of different methods for removing woody core on nystose content in Morinda officinalis[J].J TCM Univ Hunan, 2016, 36(4):31
[13] 张志鹏,梁丽金,徐杰巴,等. 巴戟天及根皮与木心UPLC特征指纹图谱与化学模式识别方法研究[J].中草药, 2020, 51(13): 3554
ZHANG ZP, LIANG LJ, XU JB, et al. Identification of Morindae Officinalis Radix root cortex and woody core based on UPLC characteristic chromatogram and chemical pattern recognition method[J].Chin Tradit Herb Drugs, 2020, 51(13): 3554
[14] 景海漪,史辑,贾天柱. 巴戟天的炮制历史沿革[J].中国药房, 2013, 24(27):2575
JING HY, SHI J, JIA TZ. The history and cultural analysis of the processing of Morinda officinalis[J].China Pharm, 2013, 24(27):2575
[15] LI B, BHANDARI DR, ROMPP A, et al. High-resolution MALDI mass spectrometry imaging of gallotannins and monoterpene glucosides in the root of Paeonialactiflora[J].Sci Rep, 2016, 6:36074
[16] LI B, BHANDARI DR, JANFELT C, et al. Natural products in Glycyrrhiza glabra(licorice) rhizome imaged at the cellular level by atmospheric pressure matrix-assisted laser desorption/ionization tandem mass spectrometry imaging[J].Plant J, 2014, 80(1): 161
[17] BECK S, STENGEL J. Mass spectrometric imaging of flavonoid glycosides and biflavonoids in Ginkgo biloba L. [J].Phytochemistry, 2016, 130: 201
[18] LI B, NEUMANN EK, GE J, et al. Interrogation of spatial metabolome of Ginkgo biloba with high-resolution matrix-assisted laser desorption/ionization and laser desorption/ionization mass spectrometry imaging[J].Plant Cell Environ, 2018, 41(11): 2693
[19] TAIRA S, IKEDA R, YOKOTA N, et al. Mass spectrometric imaging of ginsenosides localization in Panax ginseng root[J].Am J Chin Med, 2010, 38(3): 485
[20] 刘芳,赵瀚森,孙公伟, 等.基质辅助激光解析质谱成像可视化分析三七皂苷空间分布[J].分析化学,2020, 48(7): 881
LIU F, ZHAO HS, SUN GW, et al. Visualization analysis of spatial distribution of notoginsenosideby matrix-assisted laser desorption ionization mass spectrometry imaging[J].Chin J Anal Chem, 2020, 48(7): 881
[21] LI M, WANG XY, HAN LF, et al. Integration of multicomponent characterization, untargeted metabolomics and mass spectrometry imaging to unveil the holistic chemical transformations and key markers associated with wine steaming of Ligustri Lucidi Fructus[J].J Chromatogr A, 2020, 1624: 461228
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