目的:完善广西民族药材小叶金花草质量标准,提高其质量控制水平。方法:对广西境内小叶金花草进行资源调查及实地采样,收集17批次小叶金花草药材及5批混伪品大叶金花草药材,结合显微鉴别(包括根茎横切面和粉末显微)和薄层色谱鉴别对二者进行判别;参照2020年版《中华人民共和国药典》通则,对小叶金花草药材的水分、总灰分、酸不溶灰分和浸出物进行测定;建立小叶金花草中菊苣酸的HPLC测定法,采用CAPCELLPAK MGⅡC18色谱柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.3%磷酸水溶液(19:81),流速为1.0 mL·min-1,检测波长为330 nm,柱温为25 ℃。结果:与现行质量标准2018年版《广西壮族自治区壮药质量标准》第三卷相比,新增了根茎横切面显微鉴别,修订了薄层色谱鉴别方法,斑点更清晰,专属性更强;建立了菊苣酸的含量测定方法,菊苣酸质量浓度在2.73~273 μg·mL-1范围内线性关系良好(r=1.000),平均回收率为102.8%(RSD为1.1%,n=6),17批次样品菊苣酸的含量范围为0.12%~2.23%。结论:本文建立了完整的显微鉴别、薄层色谱鉴别、检查及含量测定方法,完善了小叶金花草药材的质量标准,为合理评价小叶金花草质量提供数据支撑和科学依据。
[1] 江苏新医院. 中药大辞典.上册[M].上海: 上海科学技术出版社,1992: 273
Jiangsu New Hospital. Dictionary of Traditional Chinese Pharmacy (Vol 1)[M].Shanghai:Shanghai Scientific & Technical Publishers, 1992: 273
[2] 广西壮族自治区卫生厅. 广西中药志.第二集[M].南宁: 人民出版社, 1959: 154
Guangxi Health Department.Guangxi Journal of Traditional Chinese Medicine.Part 2[M].Nanning:People's Publishing House, 1959: 154
[3] 中华人民共和国药典1977年版. 一部[S].1977: 43
ChP 1977. VolⅠ[S].1977: 43
[4] 韦松基,刘华钢,陈宇龄,等.广西本草新编[M].北京:中国医药科技出版社,2021:1810
WEI SJ, LIU HG, CHEN YL, et al. New Edition of Guangxi Materia Medica[M].Beijing:China Medical Science Press, 2021: 1810
[5] 覃迅云, 罗金裕, 高志刚. 中国瑶药学[M].南宁: 民族出版社, 2002: 537
QIN XY, LUO JY, GAO ZG. Chinese Yao Medicine[M].Nanning: National Publishing House, 2002: 537
[6] 戴斌. 中国现代瑶药[M].南宁: 广西科学技术出版社, 2009: 545
DAI B.Modern Chinese Yao Medicine[M].Nanning:Guangxi Science and Technology Press, 2009: 545
[7] 邹节明. 广西特色中草药资源选编[M].南宁: 科学出版社, 2001: 37
ZOU JM.Selection of Guangxi Characteristic Chinese Herbal Medicine Resources[M].Nanning: Science Press, 2001: 37
[8] 贾敏如, 张艺. 中国民族药辞典[M].南宁: 中国医药科技出版社, 2016: 564
JIA MR, ZHANG Y. Chinese National Medicine Dictionary[M].Nanning: China Medical Science and Technology Publishing House, 2016: 564
[9] 张雅, 王一乔, 马卓雅, 等. 药效学实验结合正交试验优选壮药柏金颗粒的水提醇沉工艺[J].中国药房, 2020, 31(8): 919
ZHANG Y, WANG YQ, MA ZY, et al. Optimization of the water extraction and ethanol precipitation technology of Zhuang medicine Baijin granules by pharmacodynamics combined with orthogonal test[J].China Pharm, 2020, 31(8): 919
[10] 方玉, 程佩佩, 夏叶, 等. 野鸡尾与易混品乌蕨的生药鉴别[J].中药材, 2016, 39(4): 753
FANG Y, CHENG PP, XIA Y, et al. Identification of Onychium japonicum (Thunb.) Kze. and Stenoloma chusanum Ching[J].J Chin Med Mater, 2016, 39(4): 753
[11] 高成芝,袁阿兴,覃凌.野鸡尾的生药鉴定[J].广西医学, 1988, 10(3): 131
GAO CZ, YUAN AX, QIN L. Pharmacognosy identification of Onychium japonicum (Thunb.)[J].Guangxi Med J, 1988, 10(3):131
[12] 邓晰朝.野雉尾金粉蕨配子体发育及其系统学意义[J].广西植物, 2016, 36(1): 96
DENG XC. Gametophyte development in Onychium japonicum and its systematic significance[J].Guihaia, 2016, 36(1): 96
[13] 朱华,周洁,颜萍花.大金花草与小金花草的显微特征比较[J].华西药学杂志, 2016, 31(5): 480
ZHU H, ZHOU J, YAN PH. Comparison of microscopic characteristics of Stenoloma chusana and Onychium japonicum[J].West China J Pharm Sci, 2016, 31(5):480
[14] 窦平,杨文利,赵冉冉,等.金粉蕨属的分子系统学研究—基于5种叶绿体DNA序列片段[J].植物研究, 2015, 35(5): 665
DOU P, YANG WL, ZHAO RR, et al. Phylogenetic analysis of Onychium based on five chloroplast DNA sequences[J].Bull Bot Res, 2015, 35(5):665
[15] XU YL, KU BI, MA YB. A cytotoxic flavanone glycoside from Onychium japonicum: structure of onychin[J].Phytochemistry, 1993, 33(2): 510
[16] 李明潺.野雉尾金粉蕨的化学成分研究[D].天津: 天津医科大学, 2007: 24
LI MC. Study on Chemical Constituents from Onychium japonicum[D].Tianjin: Tianjin Medical University, 2007: 24
[17] 李明潺.两种土家族药用植物化学成分及其生物活性研究[D].天津: 天津医科大学, 2013:10
LI MC. Study on Chemical Constituents and Their Bioactivities from Two Tujia Ethno-Medicinal Plants[D].Tianjin: Tianjin Medical University, 2013:10
[18] HASEGAWA M, AKABORI Y, AKABORI S. New indanone compounds from Onychium joponicum[J].Phytochemistry, 1974, 13(2): 509
[19] HSUE TH, WANG JL, TANG CP. The structure of onychiol B, [las,3as- (1β,1aα,3aβ,5α,5aα,6α,10aβ) l-1-[l- (hydroxymethyl) vinyl]-3a,5a, 8-trimethyl-2, 3, 3a, 4, 5, 5a, 9, 10, 10a, 10b-decahydro-1H, 6H-cyclohept[e]indene-5,6-diol: aditerpenealcohol from Onychium joponicum (Thunb.) Kunze[J].Cta Cryst, 1980,36: 2802
[20] 方玉.野鸡尾止血活性部位筛选及其化学成分研究[D].武汉: 湖北中医药大学, 2016: 28
FANG Y. Studies on Screening the Hemostatic Active Fraction and Chemical Constitutents in Active Fraction from Onychium japonicum[D].Wuhan: Hubei University of Chinese Medicine, 2016: 28
[21] HASEGAWA M, TANEYAMA M. Chicoric acid from Onychium japonicum and its distribution in the ferns[J].Bot Mag, 1973, 86(4):315
[22] 殷文帅,黄风春,张圆圆,等.不同蕨类植物提取物乙酰胆碱酯酶抑制活性的评价[J].天然产物研究与开发, 2009, 35(5): 854
YIN WS, HUANG CF, ZHANG YY, et al. Evaluation of acetylcholinesterase inhibitory activity of different plant belonging to pteridophyta extract[J].Nat Prod Res Dev, 2009, 21(5):854
[23] 吕琳,庞声航,陈永红,等.壮药解毒蕨提取物对小鼠移植性肿瘤的影响[J].时珍国医国药, 2006, 17(5): 692
Lü L, PANG SH, CHEN YH, et al. Anti-tumor effect of Jiedujue extracts of Zhuang Nationality on mice[J].Lishizhen Med Mater Med Res, 2006, 17(5):692
[24] 冯艺萍,李彬,郭力城,等.小叶金花草抗炎镇痛作用[J].中国实验方剂学杂志, 2011, 17(12): 197
FENG YP, LI B, GUO LC, et al. Anti-inflammatory action and analgesic effect of Onychium japonicum[J].Chin J Exp Tradit Med Form, 2011,17(12):197
[25] 杨德胜,罗鹤辉.土家族解毒药的临床应用[J].湖南中医药杂志, 2010, 26(6): 101
YANG DS, LUO HH. Clinical application of Tujia antidote[J].Hunan J Tradit Chin Med, 2010, 26(6): 101
