成分分析

4种市售发酵虫草菌粉胶囊寡糖成分分析*

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  • 1.澳门大学 中药质量研究国家重点实验室,澳门 999078;
    2.澳门大学 中药糖工程与检测技术联合实验室,澳门 999078;
    3.江西济民可信集团有限公司,南昌 330029
第一作者 Tel:(853)63920204;E-mail:mc05828@um.edu.mo
**李绍平 Tel:(853)88224692;E-mail:spli@um.edu.mo
赵 静 Tel:(853)88224873;E-mail:jingzhao@um.edu.mo

收稿日期: 2023-07-07

  网络出版日期: 2024-06-24

基金资助

*澳门科学技术发展基金项目(0017/2019/AKP);广东省重点领域研发计划(2020B1111110006);澳门大学科学研究基金项目(MYRG2018-00083-ICMS/MYRG2019-00128-ICMS /CPG2022-00014-ICMS)

Analysis of oligosaccharide components in four kinds of commercial fermented Cordyceps mycelia powder capsules*

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  • 1. State Key Laboratory of Quality Research in Chinese Medicine, University of Macau, Macao 999078, China;
    2. Joint Laboratory of Chinese Herbal Glyco-engineering and Testing Technology, University of Macau, Macao 999078, China;
    3. Jiangxi JeMinCare Group Co., Ltd., Nanchang 330029, China

Received date: 2023-07-07

  Online published: 2024-06-24

摘要

目的: 研究4种市售发酵虫草菌粉胶囊中的寡糖成分。方法: 首先应用高效薄层色谱(HPTLC)对4种市售发酵虫草菌粉胶囊(百令胶囊、金水宝胶囊、宁心宝胶囊和心肝宝胶囊)寡糖成分进行初步分析,展开剂为正丁醇-异丙醇-乙酸-水(6∶11∶3∶4),显色剂为苯胺-二苯胺-磷酸;再采用HPTLC-MS确定寡糖聚合度,质谱选用正离子模式,质量范围m/z 100~1 500;最后结合淀粉酶酶解-HPTLC分析确定主要糖苷键类型。结果: 4种市售发酵虫草菌粉胶囊中,除宁心宝胶囊外,其他3种发酵虫草菌粉胶囊均基本不含寡糖类成分;HPTLC-MS结果显示,宁心宝胶囊样品HPTLC中Rf=0.53、0.48、0.40、0.32、0.27、0.22和0.18处的条带相对分子质量分别约为342、342、504、666、828、991、1 153,分别对应二糖及二糖至七糖;淀粉酶酶解结果提示:宁心宝胶囊中寡糖主要是α-1,4-葡聚寡糖。结论: 4种市售发酵虫草菌粉胶囊中,仅宁心宝胶囊含相对多的寡糖类成分,且主要为α-1,4-葡聚寡糖,其产生可能与菌种和/或生产工艺有关。

本文引用格式

陈芷馨, 朱宝杰, 赵鹏, 杨明, 李绍平, 赵静 . 4种市售发酵虫草菌粉胶囊寡糖成分分析*[J]. 药物分析杂志, 2023 , 43(7) : 1129 -1134 . DOI: 10.16155/j.0254-1793.2023.07.05

Abstract

Objective: To investigate oligosaccharides in four commercial fermented Cordyceps mycelia powder capsules. Methods: HPTLC was employed for preliminary analysis of oligosaccharides in four commercially available fermented Cordyceps mycelia powder capsules (Bailing capsules, Jinshuibao capsules, Ningxinbao capsules and Xinganbao capsules). TLC developing solvent was 1-butanol-isopropanol-water-acetic acid (6∶11∶3∶4) and colorized with diphenylamine-aniline-phosphoric acid reagent. Then HPTLC-MS was applied to determine the degree of polymerization in positive ion mode with mass range of m/z 100-1 500. Finally, the major glycosidic linkages were determined in combination of amylase digestion and HPTLC analysis. Results: Among the four commercially available fermented Cordyceps mycelia powder capsules, except Ningxinbao capsules, the other three fermented Cordyceps mycelia powder capsules were almost free of oligosaccharides; HPTLC-MS results showed that the molecular mass of the bands at with Rfs of 0.53, 0.48, 0.40, 0.32, 0.27, 0.22 and 0.18 in Ningxinbao capsules were about 342, 342, 504, 666, 828, 991, 1 153, corresponding to disaccharides and disaccharides to heptasaccharides, respectively; the results of amylase digestion suggested that the oligosaccharides in Ningxinbao capsules were mainly α-1,4-gluco-oligosaccharides. Conclusion: Among the four commercially available fermented Cordyceps mycelia powder capsules, only Ningxinbao capsules contain relatively high amount of oligosaccharides, and they are α-1,4-gluco-oligosaccharides, the production of which may be derived from different strains and/or production process.

参考文献

[1] 杨平, 赵晓霞, 张永文. 发酵虫草菌类产品的标准比较与评价[J].中国中药杂志, 2018, 43(3): 463
YANG P, ZHAO XX, ZHANG YW. Comparison and review on specifications of fermented Cordyceps sinensis products[J].China J Chin Mater Med, 2018, 43(3): 463
[2] 张萍, 刘薇, 邹秦文, 等. 虫草发酵类产品质量评价与控制研究进展[J].中国药学杂志, 2021, 56(14): 1118
ZHANG P,LIU W,ZOU QW, et al. Research progress in quality evaluation and control of Cordyceps sinensis fermentation products[J].Chin Pharm J, 2021, 56(14): 1118
[3] GUAN J, ZHAO J, FENG K, et al. Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide mapping[J].Anal Bioanal Chem, 2011, 399(10): 3465
[4] GUAN J, YANG FQ, LI SP. Evaluation of carbohydrates in natural and cultured Cordyceps by pressurized liquid extraction and gas chromatography coupled with mass spectrometry[J].Molecules, 2010, 15(6): 4227
[5] 邓勇, 张杰良, 王兰英, 等. 薄层色谱法分析不同虫草多糖的单糖组成[J].药物分析杂志, 2018, 38(1): 13
DENG Y, ZHANG JL, WANG LY, et al. Analysis of monosaccharide composition in polysaccharides of Cordyceps spp. by TLC[J].Chin J Pham Anal, 2018, 38(1): 13
[6] 唐艳萍, 李敏, 余弦. 冬虫夏草及其发酵虫草中甘露醇含量比较研究[J].成都中医药大学学报, 2009, 32(4): 85
TANG YP, LI M, YU X. Comparing research on content determination of mannitol about the Cordyceps sinensis and fermentation of Cordyceps militaris[J].J Chengdu Univ Tradit Chin Med, 2009, 32(4): 85
[7] 张萍, 肖新月, 黄玮, 等. RP-HPLC-UV法测定5种发酵虫草制剂中麦角甾醇的含量[J].药物分析杂志, 2011, 31(2): 258
ZHANG P, XIAO XY, HUANG W, et al. RP-HPLC-UV determination of ergosterol in five preparations of fermental Cordyceps[J].Chin J Pham Anal, 2011, 31(2): 258
[8] ZHAO J, SHI T, ZHU W, et al. Quality control method of sterols in fermented Cordyceps sinensis based on combined fingerprint and quantitative analysis of multicomponents by single marker[J].J Food Sci, 2020, 85(10): 2994
[9] 张萍, 周玉春, 王晓, 等. HPLC柱前衍生化法测定发酵虫草制剂中总氨基酸的含量[J].药物分析杂志, 2016, 36(8): 1338
ZHANG P, ZHOU YC, WANG X, et al. Determination of amino acid in fermented Cordyceps preparation by HPLC with precolumn derivatization method[J].Chin J Pharm Anal, 2016, 36(8): 1338
[10] WEI X, XU N, WU D, et al. Determination of branched-amino acid content in fermented Cordyceps sinensis mycelium by using FT-NIR spectroscopy technique[J].Food Bioprocess Technol, 2014, 7(1): 184
[11] 曹稳, 洪亮, 杨明, 等. 发酵虫草菌粉及产品指纹图谱建立及多指标成分分析[J].色谱, 2021, 39(9): 1006
CAO W, HONG L, YANG M, et al. Fingerprint establishment and multi-indicator quantitative analysis of fermented Cordyceps powder and products[J].Chin J Chromatogr, 2021, 39(9): 1006
[12] 黄旭东, 刘伟, 徐新元, 等. 宁心宝胶囊指纹图谱研究及4种核苷类成分的含量测定[J].中国药师, 2016, 19(3): 488
HUANG XD, LIU W, XU XY, et al. Study on HPLC fingerprint and simultaneous determination of four nucleosines in Ningxinbao capsules[J].China Pharm, 2016, 19(3): 488
[13] SHI T, GUAN Y, CHEN L, et al. Application of near-infrared spectroscopy analysis technology to total nucleosides quality control in the fermented Cordyceps powder production process[J].J Anal Methods Chem, 2020: 8850437
[14] YANG FQ, LI SP. Effects of sample preparation methods on the quantification of nucleosides in natural and cultured Cordyceps[J].J Pharm Biomed Anal, 2008, 48(1): 231
[15] YANG FQ, GE LY, YONG JWH, et al. Determination of nucleosides and nucleobases in different species of Cordyceps by capillary electrophoresis-mass spectrometry[J].J Pharm Biomed Anal, 2009, 50(3): 307
[16] CHEN LH, WU Y, GUAN YM, et al. Analysis of the high-performance liquid chromatography fingerprints and quantitative analysis of multicomponents by single marker of products of fermented Cordyceps sinensis[J].J Anal Methods Chem, 2018: 5943914
[17] MUSSATTO SI, MANCILHA IM. Non-digestible oligosaccharides: a review[J].Carbohydr Polym, 2007, 68(3): 587
[18] RAHIM MA, SAEED F, KHALID W, et al. Functional and nutraceutical properties of fructo-oligosaccharides derivatives: a review[J].Int J Food Prop, 2021, 24(1): 1588
[19] ZHANG N, JIN M, WANG K, et al. Functional oligosaccharide fermentation in the gut: improving intestinal health and its determinant factors―a review[J].Carbohydr Polym, 2021: 119043
[20] MANO MCR, NERI-NUMA IA, da SILVA JB, et al. Oligosaccharide biotechnology: an approach of prebiotic revolution on the industry[J].Appl Microbiol Biotechnol, 2018, 102(1): 17
[21] 陈凌霄, 钟如此, 赵静, 等. 薄层色谱结合气相色谱-质谱法评价几种市售功能糖质量[J].药物分析杂志, 2018, 38(1): 29
CHEN LX, ZHONG RC, ZHAO J, et al. Quality evaluation of commercial functional saccharides by TLC and GC-MS[J].Chin J Pham Anal, 2018, 38(1): 29
[22] WU DT, CHEONG KL, WANG LY, et al. Characterization and discrimination of polysaccharides from different species of Cordyceps using saccharide mapping based on PACE and HPTLC[J].Carbohydr Polym, 2014, 103: 100
[23] DENG Y, CHEN CW, CHEN LX, et al. Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China[J].J Pharm Anal, 2021, 11(3): 284
[24] ZHANG J, YU H, LI S, et al. Comparative metabolic profiling of Ophiocordyceps sinensis and its cultured mycelia using GC-MS[J].Food Res Int, 2020, 134: 109241
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