Objective: To establish a quantitative analysis of multi-components by single marker(QAMS) method for simultaneous determination of 8 active components(neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, nicotiflorin, hederacoside C, hederacoside B and α-hederin) in Hedera helix. Methods: HPLC was performed on a Lubex Kromasil C18(250 mm×4.6 mm, 5 μm) column with mobile phase consisting of acetonitrile-0.1% phosphoric acid solution (gradient elution). The detection wavelength was set at 326 nm(0-15 min for phenolic acids), 266 nm(15-25 min for flavones) and 210 nm (25-45 min for saponins). The flow rate was 1.0 mL·min-1, the column temperature was 30 ℃ and the injection volume was 10 μL. Using chlorogenic acid as the internal reference, the relative correction factors(RCFs) of neochlorogenic acid, cryptochlorogenic acid, rutin, nicotiflorin, hederacoside C, hederacoside B and α-hederin were established by slope correction, multi-point correction and single-point correction. Effects of different chromatogram system, chromatogram column, mobile phase gradient proportion, determination wavelength, flow rate and column temperature on the RCFs were investigated. The effects of relative retention time(RRT) and two-point correction(TPC) on peak location were investigated. The difference between the results of QAMS, external standard method(ESM) and standard curve method(SCM) was investigated by relative error(RE), and the accuracy and feasibility of QAMS were evaluated. Results: RCFs of neochlorogenic acid, cryptochlorogenic acid, rutin, nicotiflorin, hederacoside C, hederacoside B and α-hederin relative to chlorogenic acid were 0.980, 1.019, 0.653, 0.708, 0.062, 0.064, 0.117(slope correction); 0.975, 1.006, 0.645, 0.711, 0.060, 0.061, 0.114 (multi-point correction); 0.952, 0.992, 0.631, 0.704, 0.061, 0.063, 0.112(single-point correction). RCFs reproducibility was perfect under different experiment conditions(RSD＜3%). Relative retention time and two-point correction can not be used to locate the chromatographic peak accurately, but the combination of the two methods can achieve the accurate location of chromatographic peak, relative error(RE) less than 3%. The contents of 8 components in 10 batches of Hedera helix were 0.101-0.495 mg·g-1 for neochlorogenic acid, 1.134-4.855 mg·g-1 for chlorogenic acid, 0.064-0.348 mg·g-1 for cryptochlorogenic acid, 1.054-4.558 mg·g-1 for rutin, 0.452-1.331 mg·g-1 for nicotiflorin, 11.121-72.350 mg·g-1 for hederacoside C, 4.944-14.100 mg·g-1 for hederacoside B, 5.325-19.734 mg·g-1 for α-hederin by QAMS(single-point correction). The result obtained by single point correction was consistent with that of external standard method(RE=0%). The results obtained by slope correction and multi-point correction were close to those obtained by external standard method and standard curve method(RE＜5%). Conclusion: The QAMS method is simple, rapid, accurate and reliable, it can be used for simultaneous determination of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, rutin, nicotiflorin, hederacoside C, hederacoside B and α-hederin in Hedera helix.
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