目的: 对市售维吉尼雪松精油主要挥发性成分分析,并探究其体外药理活性。方法: 采用气相-质谱联用法,色谱柱为Agilent HP-5MS(250 μm×30 m,0.25 μm),载气为高纯氦气,进样量0.2 μL;分流比10∶1;柱流量 1. 0 mL·min-1,进样口温度220 ℃。离子源为EI,离子源温度230 ℃,电离能量70 eV,质量扫描范围m/z 12～550,溶剂延迟 3 min;采用面积归一化法测定各组分的相对含量;牛津杯法测定抑菌圈的大小,评价其对金黄色葡萄球菌、大肠埃希菌及白色念珠菌的体外抑菌活性;通过建立脂多糖(lipopolysaccharide, LPS)刺激RAW264.7细胞系炎症模型,探究其对巨噬细胞相关炎症因子TNF-α表达量的影响;噻唑蓝(Methyl Thiazolyl Tetrazolium, MTT)法测定其对HT-29肿瘤细胞体外细胞毒活性。结果: 从维吉尼亚雪松精油样品中共检测出21个成分,占总峰面积的95.67%,主要成分为雪松醇(26.58%)、α-雪松烯(24.05%)、顺罗汉柏烯(23.46%)等萜烯类和萜醇类化合物;维吉尼亚雪松精油可显著提高TNF-α的表达量,呈成剂量依赖性抑制HT-29细胞的增殖,但对3个菌敏感度均不高。结论: 维吉尼亚雪松精油成分丰富,具有一定的抗菌活性,同时能促进LPS诱导RAW264.7细胞向M1型极化的作用,并有较好的抗HT-29肿瘤细胞增殖作用。
Objective: To analyze the main volatile constituents of Virginia Cedar essential oils from the market, and to explore its pharmacological activity in vitro. Methods: GC-MS method was adopted. The determination was performed on capillary column of Agilent HP-5MS (250 μm×30 m,0.25 μm), and high purity helium was used as carrier gas. The injection volume was 0.2 μL, the split ratio was 10∶1, the column flow was 1.0 mL·min-1 and the inlet temperature was 220 ℃. The ion source was EI, and its temperature was 230 ℃. The ionization energy was 70 eV, the mass scan range was m/z 12-550 and the solvent delay was 3 minutes. The relative contents of each component were determined by area normalization method. The size of inhibition zone was determined by Oxford cup method and the antibacterial activity to the Staphylococcus aureus, Escherichia coli and Candida albicans in vitro was evaluated. Lipopolysaccharide (LPS) stimulated RAW246.7 cells inflammation model was established to investigate its effect on the inflammatory factor TNF-α expression level of RAW264.7 in vitro. Its cytotoxic activity in vitro was tested on the HT-29 cells by methyl thiazolyl tetrazolium (MTT) method. Results: 21 compounds were identified in the essential oils sample of Virginia Cedar, accounting for 95.67% of the total peak area, and the main components were terpenes and alcohols, such as Cedrol (26.58%), α-Cedrene (24.05%), and cis-Thujopsene (23.46%). Virginia Cedar essential oils inhibited the tumor cell HT-29 proliferation in a dose dependent manner, and significantly increased the expression of TNF-α. But the sensitivity of the essential oils to the three strains were not high. Conclusions: The essential oils of Virginia Cedar is rich in ingredients, promoting the polarization of RAW264.7 cells model induced by LPS to M1 type and showing better anti-tumor activity to HT-29 cells.
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