目的: 研究建立化妆品中大麻二酚(CBD)及△9-四氢大麻酚(△9-THC)的检测方法,以CBD的含量作为功效参考指标,△9-THC的含量作为安全性评价指标,评价含大麻叶提取物成分化妆品的质量。方法: 以Agilent ZORBAX SB-C18(4.6 mm×250 mm, 5 μm)为色谱柱,流动相为乙腈-水,梯度洗脱,流速1.0 mL·min-1,波长220 nm,建立HPLC同时测定CBD及△9- THC含量的方法。采用该方法对20批化妆品中CBD及△9- THC含量进行测定,并在样品放置15、35、80 d后,分别对其中CBD含量进行监测。结果: CBD线性范围为0.2~100 μg·mL-1、△9-THC线性范围为0.02~10 μg·mL-1,精密度、加样回收率结果均符合要求。20批测定样品中,仅12批样品检出CBD,含量范围为0.002 6%~1.3%,含量在80 d内无明显变化;1批样品检出△9-THC,含量为0.000 2%。结论: 该方法快速、简便,适用于化妆品中CBD与△9-THC的测定,可为相关化妆品的产品开发及质量评估提供依据。部分样品存在与配方标示不符及△9-THC风险成分检出的情况,应加强对相关产品的市场监管。
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