Objective: To establish an HPLC fingerprint method of Tianlongjie capsules, and to determine the contents of salidroside, notoginsenoside R1, ginsenoside Rg1, loureirin A and loureirin B at the same time. Methods: The Diamonsil C18 column (250 mm×4.6 mm,5 μm) was used with acetonitrile-phosphoric acid aqueous solution as mobile phase for gradient elution. The detection wavelength was 203 nm, the flow rate was 1 mL·min-1, the injection volume was 10 μL, and the column temperature was 30 ℃. The fingerprints of 14 batches of Tianlongjie capsules were established by HPLC method, and the fingerprints were studied by similarity evaluation, cluster analysis, principal component analysis, orthogonal partial least square discriminant analysis and cluster heat map. Results: The HPLC patterns of 14 batches of Tianlongjie capsules had 32 common peaks, and the similarities were more than 0.9. The 14 batches of samples were divided into 4 groups by cluster analysis, principal component analysis, orthogonal partial least square discriminant analysis and cluster heat map. S1-S5 was one class, S7 and S8 were one class, S11-S14 was one class, S6, S9 and S10 were one class. Compared with the mixed reference substances, five chemical constituents were identified as salidroside, notoginsenoside R1, ginsenoside Rg1, loureirin A and loureirin B, which showed good linear relationship in the range of 100.1-329.4, 75.4-229.3, 85.9-265.1, 3.9-12.4 and 60.3-181.1 μg·mL-1, respectively. The average recovery were 98.5%-103.0%. The average contents of salidroside, notoginsenoside R1, ginsenoside Rg1, loureirin A and loureirin B were 2.497, 1.603, 2.098, 0.085 and 1.284 mg·g-1 in 14 batches of Tianlongjie capsules, respectively. Conclusion: The established fingerprint method of Tianlongjie capsules is simple, stable and reliable, and can be used for the quality control and comprehensive evaluation of Tianlongjie capsules.
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