目的:建立快速检测降糖类保健食品中13 种化学降糖药的HPLC-DAD法并用于实际检测。方法:采用甲醇超声提取样品中的降糖类化学药物,并应用高效液相色谱-二极管阵列检测器法测定其含量。色谱条件:采用Inert Sustain C18色谱柱(4.6 mm×250 mm,5 μm) 进行分离,以甲醇和10 mmol·L-1乙酸铵为流动相进行梯度洗脱,流速为1.0 mL·min-1,柱温为35 ℃,检测波长为235 nm,对13种降糖类化学药物进行定性、定量测定。结果:13种降糖类化学药物在相应的浓度范围内与其对应峰面积呈良好的线性关系,相关系数均大于0.999 8,3个浓度水平回收率(n=6)为78.6 %～111.2 %,RSD为 0.10%~4.5%。应用本法对11种宣称辅助降血糖保健食品样品进行检测,其中4种样品非法添加了降糖类化学药物,且均为复合添加;4种样品均添加了苯乙双胍、格列本脲,2种样品添加罗格列酮,1种样品添加吡格列酮。结论:该方法操作简单,分析速度快,可作为宣称辅助降血糖类保健食品中非法添加的13种化学药物的有效检测方法,能满足实验室的日常检测需求,具有实际应用意义。
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