Objective: To establish a method for the determination of 161 pesticide residues in Radix Glehniae, to understand the residual situation of medicinal materials and decoction pieces in the national circulation field, and to identify the differences of habitats statistically. Methods: The QuEChERS (quick, easy, cheap, effective, rugged and safe) method was used for sample preparation, gas chromatography-tandem mass spectrometry and liquid chromatography-tandem mass spectrometry were used to simultaneously determine the residues of 161 pesticide compounds in Radix Glehniae, and the data of pesticide residues in samples from main habitats were analyzed by statistical analysis. Results: Among 625 batches of samples, 24 kinds of pesticide and plant growth regulator residues were detected in 400 batches, the detection rate was 64.00%, and the over standard rate was 2.24%. The linear relationship among varieties was good. The average recoveries of three-level spiked standards were between 70%-120% with RSDs below 20%. The residues of banned pesticides DDT were common. Chlorpyrifos, propiconazole, profenofos, trifluralin and paclobutrazol were the dominant factors of differential expression of habitats. Conclusion: The method can be applied to the screening of pesticide residues in Radix Glehniae. In the past two years, the detection rate of agricultural residues of Radix Glehniae in China is high, the over standard rate is low, the highly toxic residue is high, and the influence of banned pesticide on soil accumulation is still. There are obvious regional differences in pesticide residues of Radix Glehniae.
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