[1] FURUYA T, ASADA Y, MORI H. Naturally Occurring Car-cinogens of Plant Origin, Toxicology, Pathology and Biochemistry[M].Oxford: Elsevier, 1987:25
[2] FU PP, XIA QS, CHOU MW, et al. Detection, hepatotoxicity, and tumorigenicity of pyrrolizidine alkaloids in Chinese herbal plants and herbal dietary supplements[J].J Food Drug Anal, 2007, 15(4):400
[3] 朱雷,花日茂,王路瑶,等. 植物中吡咯里西啶生物碱的检测分析方法研究进展[J].农产品质量与安全, 2021(4):8
ZHU L, HUA RM, WANG LY, et al. Research progress on detection and analysis methods of pyrrolizidine alkaloids in plants[J].Qual Saf Agro-Prod, 2021(4):8
[4] POPPENGA RH, PUSCHNER B. Poisonous Plant Threats to Cattle and Horses: Tansy Ragwort, common Groundsel and Fiddleneck[DB/OL].[2019-2-16].http://www.vetted.UCDavis.edu/cahfs/local-assets/pdfs/PoisonousPlantThreatsFAQ.pdf
[5] European Food Safety Authority(EFSA). Scientific opinion on pyrrolizidine alkaloids in food and feed[J].EFSA J, 2011, 9(11):2406
[6] European Food Safety Authority(EFSA). Opinion of the scientific panel on contaminants in the food chain on a request from the European commission related to pyrrolizidine alkaloids as undesirable substances in animal feed[J].EFSA J, 2007, 447:1
[7] 马跃新,冯有龙,吴嫣艳,等. 中药材中毒性吡咯里西啶类生物碱分析方法研究进展及控制现状[J].中药材, 2021, 52(24):7645
MA YX, FENG YL, WU YY, et al. Toxic pyrrolizidine alkaloids in Chinese herbal medicine: control status and advances in analytical method[J].J Chin Med Mater, 2021, 52(24):7645
[8] 昝珂,蒋黄卉,金红宇,等. 中药肝毒性吡咯里西啶生物碱的质量控制研究进展[J].药物分析杂志, 2021, 41(4):572
ZAN K, JIANG HH, JIN HY, et al. Research progress on quality control of hepatotoxic pyrrolizidine alkaloids in traditional Chinese medicine[J].Chin J Pharm Anal, 2021, 41(4):572
[9] 张燕. 佩兰中吡咯里西啶类生物碱的分析检测及体外神经毒性研究[D].北京:北京中医药大学, 2021.
ZHANG Y. Analysis and Detection of Pyrrolizidine Alkaloids in Eupatorii Herba and Study on Neurotoxicity in vitro[D].Beijing:Beijing University of Traditional Chinese Medicine, 2021
[10] CREWS C, BERTHILLER F, KRSKA R, et al. Update on analytical methods for toxic pyrrolizidine alkaloids[J].Anal Bioanal Chem, 2010, 396(1):327
[11] 程实,赵小宁,孙巍,等. 国内外中药材吡咯里西啶类生物碱控制情况浅析[J].中医药学报, 2021, 49(3):87
CHENG S, ZHAO XN, SUN W, et al. Analysis on the control of pyrrolizidine alkaloids in Chinese medicinal materials at home and abroad[J].Acta Chin Med Pharmacol, 2021, 49(3):87
[12] JEONG SH, CHOI EY, KIM J, et al. LC-ESI-MS/MS simultaneous analysis method coupled with cation-exchange solid-phase extraction for determination of pyrrolizidine alkaloids on five kinds of herbal medicines[J].J AOAC Int, 2021, 104(6):1514
[13] 张芳,王长虹,王峥涛. 植物中吡咯里西啶生物碱的检测与分析[J].天然产物研究与开发,2006, 18(6):1057
ZHANG F, WANG CH, WANG ZT. Detection and analysis of pyrrolizidine alkaloids in plants[J].Nat Prod Res Dev, 2006, 18(6):1057
[14] 张兰,高广慧,蒋晓彤,等. 液相色谱-三重四极杆质谱法测定花茶中5种吡咯里西啶类生物碱[J].食品安全质量检测学报, 2019, 10(23):7839
ZHANG L, GAO GH, JIANG XT, et al. Determination of 5 kinds of pyrrolizidine alkaloids in scented tea by liquid chromatography-triple quadrupole mass spectrometry[J].J Food Saf Qual, 2019, 10(23): 7839
[15] 陈丽华,王钧篪,乔月,等. 基于UPLC-MS/MS同时测定番泻叶中吡咯里西啶生物碱的含量[J].中国现代中药, 2019, 21(7):931
CHEN LH, WANG JC, QIAO Y, et al. Simultaneous determination of pyrrolizidine alkaloids in Sennae Folium by UPLC-MS/MS[J].Mod Chin Med, 2019,21(7):931
[16] 章豪,吴银良,朱勇,等. 超高效液相色谱-串联质谱法测定菊花15种吡咯里西啶生物碱毒素[J].浙江农业科学, 2021, 62(11):2286
ZHANG H, WU YL, ZHU Y, et al. Determination of 15 pyrrolizidine alkaloid toxins in Chrysanthemi Flos ultra-high performance liquid chromatography-tandem mass spectrometry[J].J Zhejiang Agric Sci, 2021, 62(11):2286
[17] JI YB, WANG YS, FU TT, et al. Quantitative analysis of pyrrolizidine alkaloids in Gynura procumbens by liquid chromatography-tandem quadrupole mass spectrometry after enrichment by PCX solid phase extraction[J].Int J Envir Anal Chem, 2019, 99(11):1090
[18] 乔月,陈丽华,胡宏秀,等. 基于UPLC-MS/MS同时测定款冬花中吡咯里西啶生物碱及其氮氧化物的含量[J].中国实验方剂学杂志, 2020, 26(1):155
QIAO Y, CHEN LH, HU HX, et al. Simultaneous determination of pyrrolizidine alkaloids and their nitrogen oxides in Farfarae Flos by UPLC-MS/MS[J].Chin J Exp Tradit Med Form, 2020, 26(1):155
[19] 昝珂,张睿,李耀磊,等. 款冬花中吡咯里西啶生物碱类成分质量控制研究进展[J].药物分析杂志, 2021, 41(5):753
ZAN K, ZHANG R, LI YL, et al. Research progress on quality control of pyrrolizidine alkaloids in Farfarae Flos[J].Chin J Pharm Anal, 2021, 41(5):753
[20] 李宁,姚令文,刘丽娜,等. UPLC-MS/MS法测定款冬花炮制前后3个吡咯里西啶生物碱的含量[J].药物分析杂志, 2020, 40(7):1262
LI N, YAO LW, LIU LN, et al. Determination of three main pyrrolizidine alkaloids in crude and prepared Farfarae Flos by UPLC-MS/MS method[J].Chin J Pharm Anal, 2020, 40(7): 1262
[21] 昝珂,陈翠玲,周颖,等. 超高效液相色谱串联质谱法测定木贼中4种吡咯里西啶生物[J].化学分析计量, 2021, 30(12):38
ZAN K, CHEN CL, ZHOU Y, et al. Determination of four pyrrolizidine alkaloids in Equiseti Hiemalis Herba by UPLC-MS/MS[J].Chem Anal Mgt,2021,30(12):38
[22] CHEN LH, WANG JC, GUO QL, et al. Simultaneous determination and risk assessment of pyrrolizidine alkaloids in Artemisia capillaris Thunb by UPLC-MS/MS together with chemometrics[J].Molecules, 2019, 24:1077
[23] 昝珂,周颖,李耀磊,等. 灯盏细辛中吡咯里西啶生物碱的含量分析及其肝毒性风险评估[J].中国现代中药, 2021, 23(11):1897
ZAN K, ZHOU Y, LI YL, et al. Hepatotoxic risk assessment and determination of pyrrolizidine alkaloids in Erigerontis Herba[J].Mod Chin Med, 2021, 23(11):1897
[24] 贾宁,曾绍东,陈吴海,等. 超高效液相色谱-串联质谱法测定铁皮石斛中的吡咯里西啶生物碱[J].现代食品科技, 2022, 38(2):295
JIA N, ZENG SD, CHEN WH, et al. Determination of pyrrolizidine alkaloids in Dendrobium officinale Kimura et Migo by UPLC-MS/MS[J].Mod Food Sci, 2022, 38(2):295
[25] 昝珂,周颖,李耀磊,等. 半枝莲中肝毒性成分吡咯里西啶生物碱含量分析及初步风险评估[J].药物评价研究, 2021, 44(12):2608
ZAN K, ZHOU Y, LI YL, et al. Content analysis and preliminary risk assessment of hepatotoxic pyrrolizidine alkaloids in Scutellariae Barbatae Herba[J].Drug Eval Res, 2021,44(12):2608
[26] 昝珂,周颖,李耀磊,等. 飞扬草中肝毒性吡咯里西啶生物碱的含量分析及风险评估[J].中国药学杂志, 2021, 56(22):1829
ZAN K, ZHOU Y, LI YL, et al. Risk assessment and determination of hepatotoxic pyrrolizidine alkaloids in Euphorbiae Hirtae Herba[J].Chin Pharm J, 2021, 56(22):1829
[27] 昝珂,周颖,逄瑜,等. 返魂草中肝毒性吡咯里西啶生物碱的含量分析及风险评估[J].中国药物警戒, 2021, 18(11):1039
ZAN K, ZHOU Y, PANG Y, et al. Risk assessment and determination of hepatotoxic pyrrolizidine alkaloids in Senecionis Cannabifolii Herba[J].Chin J Pharm Vigil, 2021,18(11):1039
[28] 昝珂,陈翠玲,周颖,等. 马鞭草中6个肝毒性吡咯里西啶生物碱的含量测定及风险评估[J].中国实验方剂学杂志, 2021, 27(24):157
ZAN K, CHEN CL, ZHOU Y, et al. Determination and risk assessment of 6 hepatotoxic pyrrolizidine alkaloids in Verbenae Herba[J].Chin J Exp Tradit Med Form, 2021, 27(24):157
[29] 昝珂,周颖,李耀磊,等. UPLC-MS/MS测定一点红不同部位中6种吡咯里西啶生物碱的含量[J].中国中药杂志, 2021, 46(17):4456
Zan K, Zhou Y, Li YL, et al. Simultaneous determination of six pyrrolizidine alkaloids in different parts of Emilia sonchifolia[J].China J Chin Mater Med, 2021, 46(17):4456
[30] 昝珂,李耀磊,周颖,等. UPLC-MS/MS法测定大蓟中7个吡咯里西啶生物碱的含量及初步风险评估[J].药物分析杂志, 2021, 41(10):1690
ZAN K, LI YL, ZHOU Y, et al. Determination and preliminary risk assessment of seven main pyrrolizidine alkaloids in Cirsii Japonici Herba by UPLC-MS/MS[J].Chin J Pharm Anal, 2021, 41(10):1690
[31] 韩浩蕾,姜长岭,王晨,等. 超高效液相色谱-串联质谱法测定茶饮料中15种吡咯里西啶生物碱[J].农产品质量与安全, 2021(4):18
HAN HL, JIANG CL, WANG C, et al. Determination of 15 pyrrolizidine alkaloids in tea beverages by ultra-high performance liquid chromatography-tandem mass spectrometry[J].Qual Saf Agro-Prod, 2021(4):18
[32] 夏珍珍,彭茂民,郑丹,等. 湖北省茶叶中吡咯里西啶生物碱污染概况及健康风险评估[J].农产品质量与安全, 2021(4):23
XIA ZZ, PENG MM, ZHENG D, et al. Pollution profile and health risk assessment of pyrrolizidine alkaloids in tea in Hubei Province[J].Qual Saf Agro-Prod, 2021(4):23
[33] REINHARD H, ZOLLER O. Pyrrolizidine alkaloids in tea, herbal tea and iced tea beverages- survey and transfer rates[J].Food Addit Contam A, 2021, 38(11):1914
[34] KWON Y, KOO Y, JEONG Y. Determination of pyrrolizidine alkaloids in teas using liquid chromatography-tandem mass spectrometry combined with rapid-easy extraction[J].Foods, 2021, 10:2250
[35] KALTNER F, STIGLBAUER B, RYCHLIK M, et al. Development of a sensitive analytical method for determining 44 pyrrolizidine alkaloids in teas and herbal teas via LC-ESI-MS/MS[J].Anal Bioanal Chem, 2019, 411(27):7233
[36] 昝珂,谢培德,金红宇,等. 蜂蜜中肝毒性吡咯里西啶类生物碱成分质量控制研究进展[J].药物分析杂志, 2021, 41(6):943
ZAN K, XIE PD, JIN HY, et al. Research progress on quality control of hepatotoxic pyrrolizidine alkaloids in honey[J].Chin J Pharm Anal, 2021,41(6):943
[37] 姜冰,丁涛,曹崇江,等. HPLC-MS/MS法同时测定动物源性食品中9种吡咯里西啶类生物碱的含量[J].分析测试学报, 2020, 39(4):473
JIANG B, DING T, CAO CJ, et al. Determination of 9 pyrrolizidine alkaloids in animal-derived foods by HPLC-MS/MS[J].J Instrum Anal, 2020, 39(4):473
[38] 乔月.《中国药典》收录的药材中吡咯里西啶生物碱及其氮氧化物的分析检测研究[D].北京:北京协和医学院, 2019
QIAO Y. Analysis and Detection of Pyrrolizidine Alkaloids and Nitrogen Oxides in Medicinal Materials included in “Chinese Pharmacopoeia”[D].Beijing: Peking Union Medical College, 2019
[39] KACZYNSKI P, OZOWICKA B. A novel approach for fast and simple determination pyrrolizidine alkaloids in herbs by ultrasound-assisted dispersive solid phase extraction method coupled to liquid chromatography-tandem mass spectrometry[J].J Pharm Biomed, 2020, 187:113351
[40] MARTINELLO M, BORIN A, STELLA R, et al. Development and validation of a QuEChERS method coupled to liquid chromatography and high resolution mass spectrometry to determine pyrrolizidine and tropane alkaloids in honey[J].Food Chem, 2017, 234:295
[41] 昝珂,周颖,李耀磊,等. 罗布麻叶中肝毒性成分吡咯里西啶生物碱的含量分析及其初步风险评估[J].中国药事, 2021, 35(7):6
ZAN K, ZHOU Y, LI YL, et al. Content analysis and preliminary risk assessment of hepatotoxic components of pyrrolizidine alkaloids in Apocyni veneti Folium[J].Chin Pharm Aff, 2021, 35(7):6
[42] IZCARA S, CASADO N, MORANTE-ZARCERO S, et al. A miniaturized QuEChERS method combined with ultrahigh liquid chromatography coupled to tandem mass spectrometry for the analysis of pyrrolizidine alkaloids in oregano samples[J].Foods, 2020, 9:1319
[43] FRIEDLE C, KAPP T, WALLNER K, et al. High abundance of pyrrolizidine alkaloids in bee pollen collected in July 2019 from Southern Germany[J].Envir Monitor Assess, 2022, 194(4):250
[44] 叶学敏. 新型QuEChERS方法在果蔬农残分析中的应用研究[D].杭州:浙江工业大学, 2020
YE XM. Application of Novel QuEChERS Protocols for Analysis of Pesticide Residues in Fruits and Vegetables[D].Hangzhou:Zhejiang University of Technology, 2020
[45] IZCARA S, CASADO N, MORANTE-ZARCERO S, et al. Miniaturized and modified QuEChERS method with mesostructured silica as clean-up sorbent for pyrrolizidine alkaloids determination in aromatic herbs[J].Food Chem, 2022, 380:132189
[46] 朱学多,杨义勇,于佳,等. 固相萃取中的常见问题和解决办法[J].合成材料老化与应用, 2022, 51(2):111
ZHU XD, YANG YY, YU J, et al. Common problems and solutions in solid phase extraction[J].Synthetic Mater Aging Appl, 2022, 51(2):111
[47] 朱秀焕,郝胜玉,玄红专,等. 全自动固相萃取-高效液相色谱-串联质谱法测定蜂蜜中4种吡咯里西啶类生物碱[J].食品安全导刊, 2021(22):98
ZHU XH, HAO SY, XUAN HZ, et al. Determination of four pyrrolidines alkaloids in honey by automatic solid phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry[J].Chin Food Saf, 2021(22):98
[48] 赵暮雨,韩芳,孙锦文,等. 多壁碳纳米管作为吸附剂的QuEChERS-气相色谱-四极杆飞行时间质谱快速筛查淡水产品中145种农药残留[J].分析测试学报, 2016, 35(12):1513
ZHAO MY, HAN F, SUN JW, et al. Rapid screening of 145 pesticide residues in Limnetic products by gas chromatography-quadrupole time of flight mass spectrometry and QuEChERS with multi-walled carbon nanotubes[J].J Instrumental Anal, 2016, 35(12): 1513
[49] LUO ZM, LI X, WANG L, et al. Development of UPLC-Q-TOF-MS coupled with cation-exchange solid-phase extraction method for the determination of ten pyrrolizidine alkaloids in herbal medicines[J].Anal Sci, 2019, 35(12):1317
[50] JEONG WT, LIM HB. Determination and chemical profiling of toxic pyrrolizidine alkaloids in botanical samples with UPLC-Q-TOF-MS[J].Chromatography, 2019, 82(11):1653
[51] LEON N, MIRALLES P, YUSA V, et al. A green analytical method for the simultaneous determination of 30 tropane and pyrrolizidine alkaloids and their N-oxides in teas and herbs for infusions by LC-Q-Orbitrap HRMS[J].Chromatogr A, 2022, 1666:462835
[52] HE YS, ZHU L, MA J, et al. Metabolism-mediated cytotoxicity and genotoxicity of pyrrolizidine alkaloids[J].Arch Toxicol, 2021, 95(3):1917