Objective: To establish a quantitative analysis of multi-components by single marker (QAMS) method for the simultaneous determination of five gallotannins of gallic acid (GA), 1-galloylglucose (1-GG), methylgallate (MG), 1, 2, 3, 6-tetragalloylglucose (TeGG) and 1,2,3,4,6-pentagalloylglucose (PGG) in Turkish galls and Xipayi mouth rinse. Methods: Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS) was used to identify the main gallotannins in Turkish galls and for selecting the components for quantitative analysis. HPLC was used to establish the method for simultaneous determination of the multi-components with the chromatographic conditions of Phenomenex C18 (250 mm×4.6 mm, 5 μm) column, the mobile phase of acetonitrile (A) -0.2% phosphoric acid aqueous solution (B) for gradient elution (0-20 min, 3%A→8%A;20-30 min,8%A→10%A; 30-70 min, 10%A→15%A; 70-100 min, 15%A→18%A; 100-120 min, 18%A→30%A), flow rate of 1.0 mL·min-1, column temperature of 35 ℃, and detection wavelength of 275 nm. Using GA as the internal reference substance, the relative correction factors (RCFs) of 1-GG、MG、TeGG and PGG were calculated by slope method, respectively, and their durability were examined. The contents of the above gallotannins in nine batches of Turkish galls and three batches of Xipayi mouth rinse were calculated and compared by using QAMS and external standard method (ESM), respectively, to verify the accuracy and feasibility of QAMS. Results: Five gallotannins of GA, 1-GG, MG, TeGG and PGG, with high contents and good biological activities, were qualitatively identified from Turkish galls, and were selected as the index components for HPLC quantitative analysis. The linear ranges of GA, 1-GG, MG, TeGG and PGG were 8.13-520, 6.25-400, 8.28-530, 9.00-576 and 9.00-576 μg·mL-1, respectively. The RCFs of 1-GG, MG, TeGG and PGG were 1.75, 0.95, 1.12 and 1.25, respectively. It showed good durability for different HPLC instruments, chromatographic columns, flow rates and column temperatures (RSD≤5.00%). The content ranges of GA, 1-GG, MG, TeGG and PGG in nine batches of Turkish galls by QAMS were 3.69%-5.07%, 1.26%-1.98%, 14.0%-17.4%, 19.8%-25.3% and 5.93%-9.24%, respectively. The content ranges of GA, 1-GG and MG in three batches of Xipayi mouth rinse by QAMS were 1.89-2.40, 0.0750-0.105 and 0.874-1.12 mg·mL-1, respectively. The relative errors of the content results by QAMS and ESM were less than 3.5%, and there were no significant differences. Conclusion: A QAMS method for above five gallotannins was established, using GA as the internal reference substance. The method is simple, accurate and reliable, and can save a lot of reference substances. It could be used for quantitative analysis and quality control of Turkish galls and Xipayi mouth rinse.
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