基于一测多评策略的茯苓不同部位三萜酸分析研究<sup>*</sup>

占慧慧, 黄丹, 邓丹, 韦春玲, 孟俊华, 刘媛, 崔培梧

doi:10.16155/j.0254-1793.2023.06.18

药物分析杂志 >

2023 , Vol. 43 >Issue 6: 1061 - 1074

DOI: https://doi.org/10.16155/j.0254-1793.2023.06.18

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基于一测多评策略的茯苓不同部位三萜酸分析研究^*

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1.湖南中医药大学药学院,长沙 410208;
2.国家中医药管理局中药药性与药效三级科研实验室,长沙 410208;
3.湖南中医药大学菌物药研究室,长沙 410208;
4.湖南中医药大学科技创新中心,长沙 410208

第一作者 Tel:18390867050;E-mail:2940423266@qq.com

**Tel:15243699828;E-mail:cuipeiwu@126.com

修回日期: 2023-01-12

网络出版日期: 2024-06-25

基金资助

*国家自然科学基金[81973211/C0033375];湖南省中医药科研计划项目[D2022139 (A2022005-17)];湖南省大学生创新创业训练计划项目[湘教通[2022]174号-2918];湖南中医药大学研究生创新课题[校行研字[2021]20号-50、校行研字[2022]20号-87];湖南中医药大学中药炮制传承基地开放基金[2022ZYPZ07];湖南中医药大学生物工程重点学科共同资助(校行科字[2018]3号,2022SGJJ03)

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Triterpenes analysis in different parts of Poria Cutis by QAMS^*

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1. School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;
2. Research Lab of TCM Property & Efficacy, Grade 3, National Administration of TCM, Changsha 410208, China;
3. Mycomedicine Research Lab, Hunan University of Chinese Medicine, Changsha 410208, China;
4. Institute of Innovation and Applied Research in TCM, Hunan University of Chinese Medicine, Changsha 410208, China

Revised date: 2023-01-12

Online published: 2024-06-25

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摘要

目的: 建立测定中药茯苓4个药用部位(茯苓皮、赤茯苓、白茯苓和茯神)中多个三萜酸成分(茯苓酸、茯苓新酸B、去氢土莫酸、茯苓新酸A、去氢茯苓酸和松苓新酸)的一测多评(quantitative analysis of multi-components with a single-marker, QAMS)方法,验证该方法在茯苓质量分析中的可行性与科学性。方法: 在建立的变波长-梯度洗脱HPLC方法的基础上,以茯苓酸为内参物,建立内参物与茯苓新酸B、去氢土莫酸、茯苓新酸A、去氢茯苓酸和松苓新酸的相对校正因子(f_i/s),并采用外标法(external standard method,ESM)与QAMS法分别测定不同产地茯苓4个药用部位样品中6个三萜酸成分的含量。采用Origin 2021进行相似度分析和聚类分析。结果: 方法学考察表明所建立的变波长-梯度洗脱HPLC方法准确可靠;各相对校正因子重复性良好,QAMS法与ESM测定结果之间无显著差异。相似度和聚类分析结果表明,茯苓皮样品的产地差异较大,其中云南产的茯苓皮相似度相对较好品质较均一,赤茯苓、白茯苓和茯神的产地差异较小;不同药用部位间6个茯苓三萜酸含量的差异较大,茯苓皮中三萜酸的含量最高,赤茯苓次之,其次为白茯苓和茯神;在同一药用部位的6个茯苓三萜酸含量方面,茯苓皮中松苓新酸和茯苓酸的含量相对较高,赤茯苓、白茯苓及茯神中以茯苓酸、去氢茯苓酸和去氢土莫酸占优势,赤茯苓中的茯苓新酸A含量相对最低。结论: 建立的变波长-梯度洗脱HPLC分析策略和QAMS方法可以同时评价茯苓4个药用部位中6个茯苓三萜酸成分,对于中药茯苓中的三萜成分分析和质量控制具有重要参考价值。

关键词： 茯苓; 不同药用部位; 三萜酸; 一测多评; 外标法; 高效液相色谱法

本文引用格式

占慧慧, 黄丹, 邓丹, 韦春玲, 孟俊华, 刘媛, 崔培梧 . 基于一测多评策略的茯苓不同部位三萜酸分析研究^*[J]. 药物分析杂志, 2023 , 43(6) : 1061 -1074 . DOI: 10.16155/j.0254-1793.2023.06.18

Abstract

Objective: To establish a new method for the quantitative analysis of multi-components by quantitative analysis of multi-components with a single-marker (QAMS) and to determine the main triterpenes (pachymic acid, poricoic acid B, dehydrotumulosic acid, poricoic acid A, dehydropachymic acid, dehydrotrametenolic acid) in four medicinal parts (Poriae Cutis, Rubra Poria, White Poria, Poria cum Radix Pini) of Poria cocos simultaneously, and verify the feasibility and scientificity of the method in the quality analysis of P. cocos. Methods: Based on the established variable wavelength-gradient elution HPLC method, pachymic acid was chosen as the reference for establishing the relative correction factor of poricoic acid B, dehydrotumulosic acid, poricoic acid A, dehydropachymic acid, dehydrotrametenolic acid. The contents of six components in four medicinal parts of P. cocos from different habitats were determined by both external standard method (ESM) and QAMS. Origin 2021 was used for similarity analysis and cluster analysis. Results: The established variable wavelength-gradient elution HPLC method was accurate and reliable, and the repeatability of each relative correction factor was excellent. There was no significant difference between the results calculated by QAMS method and ESM. The results of similarity and cluster analysis showed that there were great differences in the habitats of Poriae Cutis, among which, the similarity of yunnan Poriae Cutis was relatively high and the quality was relatively uniform, while the differences of the habitats of Rubra Poria, White Poria and Poria cum Radix Pini were relatively small. The content of six triterpenes varied greatly among different medicinal parts of P. cocos. The triterpenes content of Poriae Cutis was the highest, followed by Rubra Poria, White Poria and Poria cum Radix Pini. In the same medicinal part of P. cocos, the contents of six triterpenes were as follows. The contents of dehydrotrametenolic acid and pachymic acid in Poriae Cutis were relatively high, and the contents of pachymic acid, dehydropachymic acid and dehydrotumulosic acid in Rubra Poria, White Poria and Poria cum Radix Pini were relatively high, and the contents of poricoic acid A in Rubra Poria were relatively lowest. Conclusion: The established variable wavelength-gradient elution HPLC and QAMS method can simultaneously determine six triterpenes in four medicinal parts of P. cocos, which has important reference value for triterpenoid component analysis and quality control of P. cocos.

Key words： Poria cocos (Schw.) Wolf; different medicinal parts; triterpene acids; quantitative analysis of multi-components with a single marker; external standard method; HPLC

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