目的: 建立紫菀生品和蜜炙品的HPLC指纹图谱,结合化学模式识别,分析判别生品紫菀和蜜紫菀,寻找紫菀炮制前后质量差异标志物,为紫菀炮制机理提供科学参考。方法: 运用HPLC方法,Agilent Eclipse Plus C18色谱柱(250 mm×4.6 mm,5 μm),流动相为0.1%磷酸水溶液-乙腈梯度洗脱,检测波长为200 nm,采用化学模式识别中的聚类分析(HCA)、主成分分析(PCA)和正交偏最小二乘法-判别分析(OPLS-DA)对紫菀炮制前后进行差异分析。结果: 建立了紫菀生品和蜜炙品的HPLC指纹图谱,共有23个共有峰,经与对照品比对,指认出6个色谱峰,分别为绿原酸(5号峰)、异绿原酸B(8号峰)、异绿原酸A(9号峰)、槲皮素(16号峰)、山柰酚(19号峰)、紫菀酮(25号峰)。通过HCA、PCA、OPLS-DA的化学模式识别方法可将紫菀生品和蜜炙品分为2类,槲皮素、山柰酚、紫菀酮、异绿原酸等10个成分可能是紫菀炮制前后的质量差异标志物。结论: 建立的HPLC指纹图谱结合化学模式识别分析的方法准确可靠,重复性好,可用于紫菀炮制前后的质量差异分析。
Objective: To establish HPLC fingerprints of raw and honey processed Asteris Radix et Rhizoma, combined with chemical pattern recognition methods, to compare the differences of raw and honey processed Asteris Radix et Rhizoma, and to find the quality difference markers of Asteris Radix et Rhizoma before and after processing, providing scientific reference for the processing mechanism of Asteris Radix et Rhizoma. Methods: An HPLC method was established on an Agilent Eclipse Plus C18 column(250 mm×4.6 mm,5 μm)with acetonitrile-0.1% phosphoric acid as the mobile phase for gradient elution. The detection wavelength was 200 nm. The discriminant analysis of hierarchical cluster analysis (HCA), principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were employed to distinguish the differences before and after the processing of Asteris Radix et Rhizoma. Results: The fingerprints of raw and honey processed Asteris Radix et Rhizoma were established and a total of 23 common peaks were marked. By comparison with the standards, six ingredients were identified. They were chlorogenic acid (F5), isochlorogenic acid B (F8), isochlorogenic acidA (F9), quercetin (F16), kaempferol (F19) and shionone (F25). The results of chemical pattern recognitiondemonstrated that raw and honey processed Asteris Radix et Rhizoma were divided into two categories. Ten components containing quercetin, kaempferol, shionone and isochlorogenic acid A might be the quality difference markers of them. Conclusion: The established fingerprint with chemical pattern recognition method is accurate, repeatable and reliable to apply in analyzing the quality differences before and after the processing of Asteris Radix et Rhizoma.
[1] 郭伟娜, 程磊, 牛倩. 中药紫菀的本草沿革及现代资源研究现状[J].安徽农业科学, 2013, 41(24):9943
GUO WN, CHENG L, NIU Q. The herbal evolution and modern resource research status of Aster tataricus L. f.[J].J Anhui Agric Sci, 2013, 41(24):9943
[2] 中华人民共和国药典2020年版.一部[S].2020:357
ChP 2020.Vol Ⅰ[S].2020:357
[3] 任丽花, 辛蕊华, 彭文静, 等. 紫菀不同极性段提取物的药效比较[J].南方农业学报, 2015, 46(4):675
REN LH, XIN RH, PENG WJ, et al. Comparison on efficacy of different polarity sectionextract from Aster tataricus[J].J Southern Agric, 2015, 46(4):675
[4] 钱树树, 彭林, 杨映映, 等. 蜜炙紫菀水煎剂对大肠癌LOVO细胞凋亡及迁移的影响[J].中华中医药学刊, 2015, 33(9):2146
QIAN SS, PENG L, YANG YY, et al. Effects of honey-fried radix asteris decoction (HFRAD) on cell apoptosis and migration in colorectal cancer LOVO cells[J].Chin Arch Tradit Chin Med, 2015, 33(9):2146
[5] MORITA H, NAGASHIMA S, TAKEYA K, et al. Astins A and B, antitumor cyclic pentapeptides from Aster tataricus[J].Chem Pharm Bull, 1993, 41(5):992
[6] 唐小武, 刘湘新, 唐宇龙, 等. 紫菀有效成分分析及生物碱的提取与体外抑菌研究[J].中兽医医药杂志, 2006(1):16
TANG XW, LIU XX, TANG YL, et al. Analysis of effective constituents from Aster tataricus L. f. and extracting of alkaloid and its anti bacterial test in vitro[J].Chin J Vet Med, 2006(1):16
[7] SCHAFHAUSER T, JAHN L, KIRCHNER N, et al. Antitumor astins originate from the fungal endophyte Cyanodermella asteris living within the medicinal plant Aster tataricus[J].Proc Natl Acad Sci USA, 2019, 116(52):26909
[8] NG TB, LIU F, LU Y, et al. Antioxidant activity of compounds from the medicinal herb Aster tataricus[J].Comp Biochem Physiol C Toxicol Pharmacol, 2003, 136(2):109
[9] DU H, ZHANG M, YAO K, et al. Protective effect of Aster tataricus extract on retinal damage on the virtue of its antioxidant and anti-inflammatory effect in diabetic rat[J].Biomed Pharmacother, 2017, 89:617
[10] 贾志新, 王世民, 冯五金, 等. 紫菀通便利尿作用研究[J].中药药理与临床, 2012, 28(1):109
JIA ZX, WANG SM, FENG WJ, et al. Study on the defecated and diuretic function of Ziwan[J].Pharmacol Clin Chin Mater Clin Med, 2012, 28(1):109
[11] 库尔班江, 欧阳艳, 努尔买买提. 紫菀属植物化学成分及药理作用研究进展[J].中国野生植物资源, 2010, 29(2):1
KORBANJHON, OU YY, NURMAMAT. The advance on the research of chemical constituents and pharmacological actions of Aster L. plants[J].Chin Wild Plant Resour, 2010, 29(2):1
[12] 戚华文, 徐鑫, 温柔, 等.基于化学模式识别技术的枳实HPLC定量指纹图谱研究[J].分析测试学报, 2021, 40(1):72
QI HW, XU X, WEN R, et al. An HPLC fingerprint research on citrus aurantium based on chemical pattern recognition technology[J].J Instrm Anal, 2021, 40(1):72
[13] 陈琳, 唐志书, 刘妍如, 等.UPLC指纹图谱结合化学模式识别评价白芷药材质量[J].天然产物研究与开发, 2019, 31(10):1697
CHEN L, TANG ZS, LIU YR, et al. Quality control research of Angelica dahurica base on UPLC fingerprint combined with chemical pattern recognition[J].Nat Prod Res Dev, 2019, 31(10):1697
[14] 周军辉, 伍蔚萍, 谢子民, 等.紫菀药材的高效液相色谱指纹图谱与定量分析[J].中药材, 2004, 27(8):562
ZHOU JH, WU WP, XIE ZM, et al. Studies on the fingerprint and quantitative analyzes of quercetin from Aster tataricus[J].Chin Med Mat, 2004, 27(8):562
[15] 范丽芳, 王巧, 张兰桐, 等.河北道地药材紫菀的指纹图谱研究[J].中草药, 2007, 28(10):1566
FAN LF, WANG Q, ZHANG LT, et al. Fingerprints of genuine Radix Asteris from Hebei Province[J].Chin Tradit Herb Drugs, 2007, 28(10):1566
[16] 修彦凤, 程雪梅, 刘蕾, 等.不同紫菀饮片中紫菀酮的含量比较[J].上海中医药大学学报, 2006(2):59
XIU YF, CHENG XM, LIU L, et al. Comparison of shionone content in different slices of prepared Radix Asteris[J].Acta Univ Tradit Med Sin Pharmacol Shanghai, 2006(2):59
[17] 卢艳花, 戴岳, 王峥涛, 等.紫菀祛痰镇咳作用及其有效部位和有效成分[J].中草药, 1999(5):360
LU YH, DAI Y, WANG ZT, et al. Effects of Aster on expectorant and antitussive effects and its effective parts and components[J].Chin Tradit Herb Drugs, 1999(5):360
