标准研讨

HPLC-MS/MS法同时测定藤茶中5个黄酮类成分含量*

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  • 武汉海关技术中心,武汉 430050
第一作者 Tel:(710)3082090; E-mail:yechenghust@163.com

收稿日期: 2022-10-01

  网络出版日期: 2024-06-26

基金资助

* 武汉海关科研项目(2019WK005)

Simultaneous determination of five flavonoids in leaves of Ampelopsis grossedentata by HPLC-MS/MS*

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  • Wuhan Customs Technology Center, Wuhan 430050, China

Received date: 2022-10-01

  Online published: 2024-06-26

摘要

目的: 建立同时测定藤茶中5个黄酮类成分的高效液相色谱串联质谱法。方法: 采用CNW Athena C18-WP色谱柱(100 mm×2.1 mm,3 μm),以甲醇-0.1%甲酸水溶液为流动相梯度洗脱,流速为0.3 mL·min-1,柱温为30 ℃。采用电喷雾离子源,多反应监测模式,负离子检测。结果: 杨梅素、二氢槲皮素、槲皮苷、山柰酚和(-)-表儿茶素没食子酸酯分别在54.4~1 030、28.4~1 000、27.4~1 000、24.8~998、30.6~1 010 ng·mL-1范围内线性关系良好(r>0.996 8),精密度、稳定性和重复性的RSD均小于5.0%(n=6),平均加样回收率分别为96.4%、99.8%、97.7%、95.0%和100.9%,RSD分别为4.7%、1.4%、1.1%、4.3%和3.0% (n=6)。4批藤茶样品中测得的上述5个成分的含量范围分别为0.825~1.7、5.15~10.05、0.34~1.95、0~0.002、0.198~2.62 mg·g-1结论: 该方法操作简单,快速准确,灵敏度高,重复性好,可用于藤茶中5个黄酮类成分的含量测定。

本文引用格式

叶诚, 王东鹏, 黄岭岭 . HPLC-MS/MS法同时测定藤茶中5个黄酮类成分含量*[J]. 药物分析杂志, 2022 , 42(12) : 2195 -2201 . DOI: 10.16155/j.0254-1793.2022.12.17

Abstract

Objective: To establish an HPLC-MS/MS method for simultaneous determination of five flavonoids in leaves of Ampelopsis grossedentata. Methods: The separation was performed on a CNW Athena C18-WP (100 mm×2.1 mm, 3 μm) column with gradient elution of methanol-0.1% formic acid aqueous solution at a flow rate of 0.3 mL·min-1 and a column temperature of 30 ℃. Negative ion detection was adopted with electrospray ion source and multiple reaction monitoring mode. Results: The linear ranges of myricetin, taxifolin, quercetin 3-rhamnoside, kaempferol and (-)-epicatechin gallat were 54.4-1 030 ng·mL-1, 28.4-1 000 ng·mL-1, 27.4-1 000 ng·mL-1, 24.8-998 ng·mL-1 and 30.6-1 010 ng·mL-1 (r>0.996 8), respectively. The RSDs of precision, stability and repeatability tests were below 5.0% (n=6), the average recoveries were 96.4%, 99.8%, 97.7%, 95.0% and 100.9% with RSDs of 4.7%, 1.4%, 1.1%, 4.3% and 3.0% (n=6), respectively. The average contents of the above 5 components in 4 samples were 0.825-1.7, 5.15-10.05, 0.34-1.95, 0-0.002 and 0.198-2.62 mg·g-1, respectively. Conclusion: The method is simple, rapid, accurate, sensitive and repeatable, which can be used for the determination of five flavonoids in leaves of Ampelopsis grossedentata.

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