综述专论

基于LC-MS的生物样品中胰岛素分析方法研究进展*

展开
  • 1.中国人民公安大学侦查学院,北京 100038;
    2.北京市公安司法鉴定中心法庭毒物分析公安部重点实验室,北京 100192
第一作者 Tel:15910780180; E-mail:lijiarui_1118@126.com
**张文芳 Tel:13911208706; E-mail:13911208706@139.com
王继芬 Tel:13691409863; E-mail:wangjifen58@126.com

收稿日期: 2021-04-27

  网络出版日期: 2024-06-26

基金资助

*公安部科技强警基础工作专项(2021JC31)

Research progress on LC-MS based analytical methods of insulin in biological samples*

Expand
  • 1. School of Investigation, People’s Public Security University of China, Beijing 100038, China;
    2. Key Laboratory of Forensic Toxicology, Ministry of Public Security, Beijing 100192, China

Received date: 2021-04-27

  Online published: 2024-06-26

摘要

胰岛素是调节碳水化合物代谢的重要蛋白质激素,糖尿病与胰岛素水平的异常有关。胰岛素的分析检测在法庭科学毒物分析、运动员兴奋剂监控及临床诊断等方面具有重要意义。液相色谱质谱联用技术 (LC-MS) 为生物样品中胰岛素的分析研究提供了1种灵敏且特异性高的有效方法。本文在简要介绍胰岛素分析方法的基础上,重点讨论了液相色谱质谱联用技术在相关工作中的研究现状,并对其应用过程中的局限性及发展前景进行了总结和展望,以期为法庭科学相关技术人员在进行胰岛素分析时提供参考和依据,从而推动胰岛素分析技术在相关领域的不断创新与发展。

本文引用格式

李佳瑞, 张文芳, 王继芬 . 基于LC-MS的生物样品中胰岛素分析方法研究进展*[J]. 药物分析杂志, 2022 , 42(3) : 361 -369 . DOI: 10.16155/j.0254-1793.2022.03.01

Abstract

Insulin is an important protein hormone that regulates carbohydrate metabolism. Diabetes is related to abnormal insulin levels. The analysis of insulin is essential in forensic science toxicology analysis, stimulant monitoring and clinical diagnosis. Liquid chromatography-mass spectrometry (LC-MS) provides a sensitive and highly specific method for the analysis and research of insulin in biological samples. Based on a brief introduction of insulin analysis methods, this paper focuses on the research status of LC-MS technology in related work, summarizes the limitations and prospects the development of its application. It is expected to provide reference and basis for relevant technical personnel when conducting insulin analysis, and continuously promote the innovation and development of insulin analysis technology in related fields.

参考文献

[1] BATALIS NI, PRAHLOW JA. Accidental insulin overdose[J].J Forensic Sci, 2004, 49(5):1117
[2] NIKOLIĆ S, ATANASIJEVIĆ T, POPOVIĆ V. A suicide by insulin injection—case report[J].Srp Arh Celok Lek, 2006, 134(9-10):444
[3] TONG F, WU R, HUANG W, et al. Forensic aspects of homicides by insulin overdose[J].Forensic Sci Int, 2017, 278:9
[4] BIRKINSHAW VJ, GURD MR, RANDALL SS, et al. Investigations in a case of murder by insulin poisoning[J].British Med J, 1958, 5094(2):463
[5] MARKS V. Murder by insulin:suspected, purported and proven—a review[J].Drug Test Anal, 2009, 1(4):162
[6] LU M, INBORIBOON PC. Lantus insulin overdose:a case report[J].J Emerg Med, 2011, 41(4):374
[7] MYERS AK, TRIVEDI MH. Death by insulin:management of selfharm and suicide in diabetes management[J].Curr Diabetes Rev, 2017, 13(3):251
[8] SHAH VN, FORLENZA GP. Suicide via intentional insulin overdose through first commercial hybrid closed-loop[J].J Diabetes Sci Technol, 2020, 14(3):687
[9] BARNARD-KELLY KD, NARANJO D, MAJIDI S, et al. Suicide and selfinflicted injury in diabetes:a balancing act[J].J Diabetes Sci Technol, 2020, 14(6):1010
[10] HO EN, WAN TS, WONG AS, et al. Doping control analysis of insulin and its analogues in equine urine by liquid chromatography-tandem mass spectrometry[J].J Chromatogr A, 2011, 1218(8):1139
[11] SONKSEN PH. Insulin, growth hormone and sport[J].J Endocrinol, 2001, 170(1):13
[12] THEVIS M, THOMAS A, SCHÄNZER W. Insulin[J].Handb Exp Pharmacol, 2010, 195(10):209
[13] YAO P, LIU Z, TUNG S, et al. Fully automated quantification of insulin concentration using a microfluidic-based chemiluminescence immunoassay[J].J Lab Autom, 2016, 21(3):387
[14] 自学华. 化学发光法检测2型糖尿病患者血清胰岛素与C肽水平临床价值评价[J].糖尿病新世界, 2019, 22(17):58
Zi XH. Evaluation of the clinical value of chemiluminescence detection of serum insulin and C-peptide levels in patients with type 2 diabetes[J].New World Diabetes, 2019, 22(17):58
[15] 陈超, 彭波, 李基. 人胰岛素定量测定试剂盒化学发光免疫分析法的建立及方法学评价[J].分子诊断与治疗杂志, 2015, 7(5):313
CHEN C, PENG B, LI J. Establishment and methodology evaluation of chemiluminescence immunoassay kit for the quantitative determination of human insulin[J].J Mol Diagn Ther, 2015, 7(5):313
[16] CARSLAKE HB, PINCHBECK GL, MCGOWAN CM. Evaluation of a chemiluminescent immunoassay for measurement of equine insulin[J].J Vet Int Med, 2017, 31(2):568
[17] WARNKEN T, HUBER K, FEIGE K. Comparison of three different methods for the quantification of equine insulin[J].BMC Vet Res, 2016, 12(1):1
[18] MANLEY SE, STRATTON IM, CLARK PM, et al. Comparison of 11 human insulin assays:implications for clinical investigation and research[J].Clin Chem, 2007, 53(5):922
[19] VUOJOLA J, SOUKKA T. Luminescent lanthanide reporters:new concepts for use in bioanalytical applications[J].Methods Appl Fluoresc, 2014, 2(1):12001
[20] EVEN MS, SANDUSKY CB, BARNARD ND, et al. Development of a novel ELISA for human insulin using monoclonal antibodies produced in serum-free cell culture medium[J].Clin Biochem, 2007, 40(1-2):98
[21] BLACKBURN M. Advances in the quantitation of therapeutic insulin analogues by LC-MS/MS[J].Bioanalysis, 2013, 5(23):2933
[22] BANTING FG, BEST CH. The internal secretion of the pancreas[J].1922. Indian J Med Res, 2007, 125(3):251
[23] RYLE AP, SANGER F, SMITH LF, et al. The disulphide bonds of insulin[J].Biochem J, 1955, 60(4):541
[24] MAYER JP, ZHANG F, DIMARCHI RD. Insulin structure and function[J].Biopolymers, 2007, 88(5):687
[25] ROSENFELD L. Insulin:discovery and controversy[J].Clin Chem, 2002, 48(12):2270
[26] AHMAD K. Insulin sources and types:a review of insulin in terms of its mode on diabetes mellitus[J].J Tradit Chin Med, 2014, 34(2):234
[27] RÖDER PV, WU B, LIU Y, et al. Pancreatic regulation of glucose homeostasis[J].Expert Mol Med, 2016, 48(3):e219
[28] OZA MJ, KULKARNI YA. Formononetin treatment in type 2 diabetic rats reduces insulin resistance and hyperglycemia[J].Front Pharmacol, 2018,18(9):739
[29] MANE K, CHALUVARAJU K, NIRANJAN M, et al. Review of insulin and its analogues in diabetes mellitus[J].J Basic Clin Pharm, 2012, 3(2):283
[30] SATOH T. Molecular mechanisms for the regulation of insulin-stimulated glucose uptake by small guanosine triphosphatases in skeletal muscle and adipocytes[J].Int J Mol Sci, 2014, 15(10):18677
[31] HAN HS, KANGG, KIM JS, et al. Regulation of glucose metabolism from a liver-centric perspective[J].Expert Mol Med, 2016, 48(3):e218
[32] OWEN WE, ROBERTS WL. Cross-reactivity of three recombinant insulin analogs with five commercial insulin immunoassays[J].Clin Chem, 2004, 50(1):257
[33] EVANS M, SCHUMM DPM, VORA J, et al. A review of modern insulin analogue pharmacokinetic and pharmacodynamic profiles in type 2 diabetes:improvements and limitations[J].Diabetes Obes Metab, 2011, 13(8):677
[34] TIBALDI JM. Evolution of insulin:from human to analog[J].Am J Med, 2014, 127(10):S25
[35] 黄光照, 汪德文. 法医毒理学[M].北京:人民卫生出版社, 2005:147
HUANG GZ, WANG DW. Forensic Toxicology[M].Beijing:People’s Medical Publishing House, 2005:147
[36] SALEH AAJ, LEE JJ, RAHMAN MA. Electrochemical sensors based on carbon nanotubes[J].Sensors (Basel), 2009, 9(4):2289
[37] RAFIEE B, FAKHARI AR. Electrocatalytic oxidation and determination of insulin at nickel oxide nanoparticles-multiwalled carbon nanotube modified screen printed electrode[J].Biosens Bioelectron, 2013, 15(46):130
[38] 蒲颖. 高胰岛素处理相关核酸适体的筛选及基于核酸适体技术的胰岛素检测[D].长沙:中南大学, 2011:64
PU Y. Screening of Aptamers Related to High Insulin Treatment and Insulin Detection Based on Aptamer Technology[D].Changsha:Central South University, 2011:64
[39] 沈枫林, 熊悦婷, 刘晓慧, 等. 基于质谱的蛋白质绝对定量研究策略和建议[J].质谱学报, 2021, 42(2):101
SHEN FL, XIONG YT, LIU XH, et al. Research strategies and recommendations for absolute quantification of proteins based on mass spectrometry[J].J Chin Mass Spectrom Soc, 2021, 42(02):101
[40] 姜利英, 肖小楠, 周鹏磊, 等. 基于氧化石墨烯荧光适体传感器的胰岛素检测[J].分析化学, 2016, 44(2):147
JIANG LY, XIAO XN, ZHOU PL, et al. Insulin detection based on graphene oxide fluorescent aptamer sensor[J].Chin J Anal Chem, 2016, 44(2):147
[41] LI H, LIU X, LI L, et al. CMOS electrochemical instrumentation for biosensor microsystems:a review[J].Sensors (Basel), 2016, 17(1):74
[42] XU Y, PROHN M, CAI X, et al. Direct comparison of radioimmunoassay and LC-MS/MS for PK assessment of insulin glargine in clinical development[J].Bioanalysis, 2014, 6(24):3311
[43] DONG S, GU Y, WEI G, et al. Direct comparison of LC-MS/MS and RIA methods for the pharmacokinetics assessment of human insulin in preclinical development[J].Biomed Chromatogr, 2018, 32(11):e4323
[44] 杨彩玲, 张海霞, 刘满仓. 胰岛素的色谱分析方法[J].分析科学学报, 2006, 22(3):349
YANG CL, ZHANG HX, LIU MC. The chromatographic analysis method of insulin[J].J Anal Sci, 2006, 22(3):349
[45] 盛希群, 高秋华, 徐辉碧. 体内胰岛素的测定[J].分析科学学报, 2001,17 (4):341
SHENG XQ, GAO QH, XU HB. Determination of insulin in the body[J].J Anal Sci, 2001, 17(4):341
[46] NEDELKOV D, NIEDERKOFLER EE, ORAN PE, et al. Top-down mass spectrometric immunoassay for human insulin and its therapeutic analogs[J].J Proteom, 2018, 175:27
[47] SCHMUDLACH A, FELTON J, CIPOLLA C, et al. Sample preparation protocol for bottom-up proteomic analysis of the secretome of the islets of Langerhans[J].Analyst, 2016, 141(5):1700
[48] CHEN Z, CAULFIELD MP, MCPHAUL MJ, et al. Quantitative insulin analysis using liquid chromatography-tandem mass spectrometry in a high-throughput clinical laboratory[J].Clin Chem, 2013, 59(9):1349
[49] 张家莹, 李黎, 李静, 等. 比格犬血浆中德谷胰岛素浓度检测方法的建立及应用[J].中南药学, 2020, 18(6):947
ZHANG JY, LI L, LI J, et al. Establishment and application of a method for detecting the concentration of insulin glutathione in the plasma of beagle dogs[J].Cent South Pharm, 2020, 18(6):947
[50] WUNDER C, KAUERT GF, TOENNES SW. Factors leading to the degradation/loss of insulin in postmortem blood samples[J].Forensic Sci Int, 2014, 241:173
[51] 崔艳丽, 武峰. LC-MS/MS法同时测定比格犬血浆中甘精胰岛素及其代谢物浓度[J].中国临床药理学杂志, 2018, 34(17):2117
CUI YL, WU F. Simultaneous determination of insulin glargine and its metabolites in Beagle dog plasma by LC-MS/MS method[J].Chin J Clin Pharmacol, 2018, 34(17):2117
[52] CHAMBERS EE, FOUNTAIN KJ, SMITH N, et al. Multidimensional LC-MS/MS enables simultaneous quantification of intact human insulin and five recombinant analogs in human plasma[J].Anal Chem, 2014, 86(1):694
[53] VAN DGJG, WONG S, et al. Quantitation of insulin analogues in serum using immunoaffinity extraction, liquid chromatography, and tandem mass spectrometry[J].Methods Mol Biol, 2016, 1378:119
[54] THEVIS M, THOMAS A, DELAHAUT P, et al. Qualitative determination of synthetic analogues of insulin in human plasma by immunoaffinity purification and liquid chromatography-tandem mass spectrometry for doping control purposes[J].Anal Chem, 2005, 77(11):3579
[55] THEVIS M, THOMAS A, DELAHAUT P, et al. Doping control analysis of intact rapid-acting insulin analogues in human urine by liquid chromatography-tandem mass spectrometry[J].Anal Chem, 2006, 78(6):1897
[56] MAZZARINO M, SENOFONTE M, MARTINELLI F, et al. Detection of recombinant insulins in human urine by liquid chromatography-electrospray ionization tandem mass spectrometry after immunoaffinity purification based on monolithic microcolumns[J].Anal Bioanal Chem, 2019, 411(30):8153
[57] HESS C, THOMAS A, THEVIS M, et al. Simultaneous determination and validated quantification of human insulin and its synthetic analogues in human blood serum by immunoaffinity purification and liquid chromatography-mass spectrometry[J].Anal Bioanal Chem, 2012, 404(6-7):1813
[58] GREEN RP, HOLLANDER AS, THEVIS M, et al. Detection of surreptitious administration of analog insulin to an 8-week-old infant[J].Pediatrics, 2010, 125(5):e1236
文章导航

/