成分分析

基于主成分分析和判别分析的广地龙产地溯源研究*

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  • 1.江西省农业科学院,南昌 330200;
    2.江西省分析测试研究所,南昌 330029
第一作者 Tel:13870074849; E-mail:376101430@qq.com
**Tel:13870077749; E-mail:startly1986@163.com

修回日期: 2022-02-15

  网络出版日期: 2024-06-26

基金资助

*江西省重点研发项目(GYLYZP102)

Provenance traceability of earthworm based on the principal component analysis and discriminant analysis*

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  • 1. Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China;
    2. Analysis and Testing Research Institution of Jiangxi Province, Nanchuang 330029, China

Revised date: 2022-02-15

  Online published: 2024-06-26

摘要

目的: 通过测定不同产地的广地龙中多种金属元素含量,运用主成分分析和判别分析准确溯源广地龙的生产产地。方法: 采用电感耦合等离子体发射光谱(ICP-OES)和电感耦合等离子体质谱(ICP-MS)对6个产地60个广地龙(又称参环毛蚓)样品(安徽AH,河南商水HNSH,江苏盐城JSYC,上海奉新SHFX,上海康桥SHKQ,上海泥城SHNC)中的25种元素含量进行了测定,并通过SPSS26软件对结果进行标准化,消除各元素间量纲差异,再对其进行主成分分析。结果: 可以将25个指标元素提取5个主成分,方差贡献率达84.6%,基本保留了原变量的大部分信息。以5个主成分为原始变量,建立判别典型函数,利用判别典型函数可以对6个不同产地的60个广地龙样品100%进行正确判别。结论: 该方法可为不同产地的广地龙溯源研究提供新方法。

本文引用格式

吴海燕, 徐芝亮 . 基于主成分分析和判别分析的广地龙产地溯源研究*[J]. 药物分析杂志, 2022 , 42(3) : 387 -393 . DOI: 10.16155/j.0254-1793.2022.03.04

Abstract

Objective: To trace the production area of earthworm (Pheretima aspergillum) acfarely by determining the content of various metal elements in earthworm from different areas and using principal component analysis and discriminant analysis. Methods: Metal elements in earthworm samples were profiled by ICP-OES and ICP-MS. Twenty-five elements in sixty earthworm samples from six regions (Anhai (AH), Henanshangshui (HNSH), Jiangsuyancheng(JSYC), Shanghaifengxin (SHFX), Shanghaikangqiao(SHKQ), Shanghainicheng(SHNC) were determined. SPSS 26 software was used to standardize the set of data followed by principal component analysis (PCA). Results: Five principal components were selected from 25 indexes and explained 84.6% of variance. Five typical discriminant functions were established from five principal components. The discriminant functions established could be used to discriminate 60 earthworm samples from six different origins with 100% rate. Conclusion: This method provides a new way for the traceability of earthworm from different producing areas.

参考文献

[1] 黄庆,李志武,马志国,李荷,毛润乾.地龙的研究进展[J].中国实验方剂学杂志,2018, 24(13):220
HUANG Q, LI ZW, MA ZG, et al. Research progress on Pheretima[J].Chin J Exper Pharm, 2018, 24(13):220
[2] 刘秀艳.地龙的药理研究[J].辽宁中医杂志,2008, 35(1):106.
LIU XY. Pharmacological studies on the earthworm[J].Liaoning J Traditl Chin Med, 2008, 35(1):106
[3] 杜航,孙佳明,郭晓庆,等.地龙的化学成分及药理作用[J].吉林中医药,2014, 34(7):707
DU H, SUN JM, GUO XQ, et al. Chemical constituents and pharmacological action of Dionysus digitalis [J].Jilin J Tradit Chin Med, 2014, 34(7):707
[4] 黄敬文,高宏伟,段剑飞.地龙的化学成分和药理作用研究进展[J].中医药导报,2018, 24(12):104
HUANG JW,GAO HW,DUAN JF. Advances in the study of chemical constituents and pharmacological action of Digilaria dipteris[J].Guiding J Tradit Chin, 2018, 24(12):104
[5] EUE I, KAUSCHKE E, MOHRIG W, et al. Isolution and characterization of earthworm Hemolysins and Agg Lutinins[J].Dev Comp Immund, 1998, 22(1):23
[6] 李中阳,吕文英,黄爱华,等.不同品种地龙中微量元素及重金属元素含量分析[J].微量元素与健康研究,2010,27(6):14
LI ZY,LÜ WY, HUANG AH, et al. Analysis on contents of trace elements and heavy metals in different species of Digilaria[J].Micronut Health Res, 2010, 27(6):14
[7] 李文兵,周亦农,张成光,等.5种中药重金属的检测研究[J].现代医药卫生,2010,26(16):2423
LI WB, ZHOU YN, ZHANG CG, et al. Study on detection of heavy metals in 5 kinds of traditional Chinese medicine[J].Mod Med Health, 2010, 26(16):2423
[8] 李晓东.不同品种地龙中微量元素及重金属元素含量分析[J].黑龙江科技信息,2016, 28):94
LI XD. Analysis on contents of trace elements and heavy metals in different Species of Digilaria[J].Heilongjiang Sci Technol Inf, 2016 (28):94
[9] 李勇,丁冠华.基于ICP-MS法对地龙、水蛭中重金属及有害元素的测定及分析[J].辽宁中医杂志,2018, 45(10):2152
LI Y, DING GH. Determination and analysis of heavy metals and harmful elements in earthworm and leech by ICP-MS[J].Liaoning J Tradit Chin Med, 2018, 45(10):2152
[10] 李耀磊,左甜甜,徐健,等.基于ICP-MS法对4种动物源性药材中16种无机元素的测定及量变规律研究[J].中文科技资料目录-中草药,2020, 43(2):248
LI YL, ZUO TT, XU J, et al. Determination and quantitative variation of 16 inorganic elements in 4 kinds of animal medicinal materials by ICP-MS method[J].Chin Sci Technol Inf Catal-Chin Herb Mede, 2020, 43(2):248
[11] 高华宏,刘昌,王文琼,等.正交试验法优选广地龙提取工艺[J].湖北中医学院学报,2004,6(3):31
GAO HH,LIU C,WANG WQ, et al. Orthogonal test was used to optimize the extraction process of Digitalis magnolia[J].J Hubei Coll Tradit Chin Mede, 2004,6(3):31
[12] 卓焱,廖泽勇.地龙的定性鉴定及蛋白质含量测定[J].新药研发,2014,5(3):18
ZHUO Y, LIAO ZY. Qualitative identification and determination of protein content of earthworm[J].New Drug Res Devel, 2014, 5(3):18
[13] 李廷利,李玉萍,冯翰.地龙的化学成分与临床应用概况[J].黑龙江医药,2006,19(4):303
LI TL, LI YP, FENG H. Chemical constituents and clinical application of Digitalis[J].Heilongjiang Med J, 2006, 19(4):303
[14] 吕金胜,吴畏,孟德胜,等.地龙醇提物抗炎及镇痛作用的研究[J].中国药师,2003,6(1):16
LÜ JS, WU W, MENG DS, et al. Study on anti-inflammatory and analgesic effects of ethanol extract of earthworm[J].Chine Med, 2003, 6(1):16
[15] KELLY S, BAXTER M, CHAPMAN S, et al. The application of isotopic and elemental analysis to determine the geographical origin of premium long grain rice[J].Eur Food Res Technol, 2002, 214(1):72
[16] 张玥,王朝辉,张亚婷,等. 基于主成分分析和判别分析的大米产地溯源[J].中国粮油学报,2016,31(4):1
ZHANG Y, WANG CH, ZHANG YT, et al. Rice origin tracing based on principal component analysis and discriminant analysis[J].J Chin Cereals Oils Assoc, 2016, 31(4):1
[17] 兴丽,赵凤敏,曹有福,等.不同产地亚麻籽矿物质元素及脂肪酸组成的主成分分析[J].光谱学与光谱分析,2014,34[9]:2538
XING L, ZHAO FM, CAO YF, et al. Principal component analysis of mineral elements and fatty acid composition of flaxseed from different producing areas[J].Spectrosc Spect Anal, 2014, 34[9]:2538
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