目的: 建立超高效液相色谱-三重四极杆质谱法(UPLC-MS/MS)同时测定芍药中15个成分含量。方法: 采用Waters UPLC HSS T3 (150 mm×2.1 mm,1.8 μm)色谱柱,以乙腈(A)-5 mmol·L-1乙酸铵+0.1%甲酸水溶液(B)为流动相,梯度洗脱(0~1.0 min,10%A→15%A;1.0~6.0 min,15%A→30%A;6.0~10.0 min,30%A→90%A;10.0~12.0 min,90%A→10%A),流速0.30 mL·min-1,电喷雾离子源(ESI),正、负离子模式下扫描,采用多重反应监测模式(MRM)。结果: 15个成分在考察的浓度范围内呈良好的线性关系(r＞0. 995),检测下限范围为0.039 08~25.45 mg·kg-1, 定量下限范围为0.130 3~84.83 mg·kg-1,回收率为90.6%~96.0%,RSD为2.7%~5.4%。结论: 所建立方法操作简便,分析速度快,灵敏度高,重复性、回收率良好,可用于芍药中多个成分的含量测定。
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