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巴戟天饮片及其混伪品的鉴别研究

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  • 中国食品药品检定研究院,北京 102629
第一作者 Tel:(010)53852067;E-mail:qiaofei@nifdc.org.cn
*郑 健 Tel:(010)53852080;E-mail:zhengjian@nifdc.org.cn
马双成 Tel:(010)53852076;E-mail:masc@nifdc.org.cn

收稿日期: 2021-10-18

  网络出版日期: 2024-06-26

Identification study on Morindae Officinalis Radix decoction pieces and the fakes

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  • National Institutes for Food and Drug Control, Beijing 102629, China

Received date: 2021-10-18

  Online published: 2024-06-26

摘要

目的: 基于DNA条形码技术、传统经验鉴别和理化鉴别技术区分巴戟天饮片及其混伪品。方法: 通过聚合酶链式反应(PCR),对巴戟天的内部转录间隔区2(ITS2)序列进行扩增;通过与GenBank数据库进行BLAST比对,以及利用构建NJ树的方法推断巴戟天饮片及其混伪品的基原;比较巴戟天饮片及其混伪品的种内、种间差异;比较巴戟天饮片及其混伪品的性状和薄层色谱行为差异。结果: 巴戟天饮片及其混伪品经NJ树聚类分析和与GenBank进行BLAST比对,结果显示巴戟天饮片与其混伪品分支明显,11批巴戟天混伪品被鉴定到7个种,分别为印度羊角藤(羊角藤)、蔓虎刺、硃砂根、木通、黑老虎、合蕊五味子(铁箍散)、南五味子;种内、种间变异分析结果显示,5批巴戟天饮片的种内遗传距离为0.000,巴戟天饮片与其他7个混伪品的最小种间遗传距离为0.037;巴戟天饮片及其混伪品的性状和薄层色谱行为存在差异。结论: 基于DNA条形码技术可有效区分巴戟天饮片及其混伪品,为巴戟天饮片正伪品的鉴别以及标准提升工作提供了一定的依据。

本文引用格式

乔菲, 刘杰, 房文亮, 过立农, 戴胜云, 连超杰, 马双成, 郑健 . 巴戟天饮片及其混伪品的鉴别研究[J]. 药物分析杂志, 2022 , 42(4) : 668 -675 . DOI: 10.16155/j.0254-1793.2022.04.14

Abstract

Objective: To distinguish Morindae Officinalis Radix decoction pieces and the fakes based on DNA barcoding technology, traditional identification and physicochemical identification technology. Methods: Internal transcribed spacer 2(ITS2) sequences of Morindae Officinalis Radix decoction pieces and the fakes were amplified with polymerase chain reaction(PCR).Through the method of BLAST comparison with GenBank database and establishment of NJ Tree, the origin of Morindae Officinalis Radix decoction pieces and the fakes was identified.The intra-species and inter-species differences among Morindae Officinalis Radix decoction pieces and the fakes were compared. And the differences among Morindae Officinalis Radix decoction pieces and the fakes in the properties of pharmacognosy characteristics and TLC behavior were compared. Results: The results of BLAST comparison with GenBank database and establishment of NJ Tree showed that Morindae Officinalis Radix decoction pieces and the fakes had obvious branches, and the 11 batches of fakes were identified as 7 species, including Morinda umbellata, Mitchella undulata, Ardisia crenada, Akebia quinata, Kadsura coccinea, Schisandra propinqua and Kadsura longipedunculata. Conclusion: Morindae Officinalis Radix decoction pieces and the fakescan be distinguished effectively based on the method of DNA barcoding technology. It provides a basis for the identification and standard improvement of Morindae Officinalis decoction pieces.

参考文献

[1] 中华人民共和国药典2020年版. 一部[S].2020:63
ChP 2020.Vol Ⅰ[S].2020:63
[2] 李鑫洁,李玲玲,鲁艺. 巴戟天寡糖提纯工艺及药理作用研究进展[J].天然产物研究与开发,2019,31(2):345
LI XJ, LI LL, LU Y.The research of Morinda officinalis How’s oligosaccharide extraction,purification and pharmacological effects[J].Nat Prod Res Dev, 2019,31(2):345
[3] 周妍妍,周晓洁,闫博文,等.巴戟天化学成分及药理作用的研究进展[J/OL].辽宁中医药大学学报, 2021:1[2021-08-05]. http://kns.cnki.net/kcms/detail/21.1543.R.20210413.1035.008.html
ZHOU YY,ZHOU XJ,YAN BW,et al. Chemical components and pharmacological effect of Morindae Officinalis Radix[J/OL].J Liaoning Univ Tradit Chin Med, 2021:1 [2021-08-05]. http://kns.cnki.net/kcms/detail/21.1543.R.20210413.1035.008.html
[4] 段宙位,何艾,陈婷,等.巴戟天多糖的理化性质及其对成骨细胞MC3T3-E1增殖的影响[J].食品工业科技,2019,40(23):312
DUAN ZW, HE A, CHEN T, et al. Physicochemical properties of polysaccharidesfrom Morinda officinalis How and its influenceon the proliferation of osteoblasts MC3T3-E1 cells[J].Sci Technol Food Ind, 2019,40(23):312
[5] ZHANG JH, XIN HL, XU YM,et al. Morinda officinalis How. ——a comprehensive review of traditional uses, phytochemistry and pharmacology[J].J Ethnopharmacol, 2018, 213:230
[6] 蔡月琴,刘艺敏,凡莉莉,等. 响应面法优化巴戟天多糖提取工艺[J].森林与环境学报,2019,39(3):303
CAI YQ, LIU YM, FAN LL, et al. Optimization of extraction of poiysaccharide from Morinda officinalis by response surface methodology[J].J Forest Environ, 2019, 39(3):303
[7] 运锋. 巴戟天抗抑郁作用现代药理研究进展[J].辽宁中医药大学学报,2019, 21(10):126
YUN F. Development of modern pharmacological research on the antidepressant effects of fuphordolitids[J].J Liaoning Univ TCM,2019, 21(10):126
[8] 宋开蓉, 高建德, 刘雄, 等. 巴戟天现代研究进展[J].中兽医医药杂志, 2018, 37(3):79
SONG KR, GAO JD, LIU X, et al. Modern research progress of Morinda officinalis[J].J Tradit Chin Vet Med, 2018, 37(3):79
[9] 赖满香, 冯娟, 阮志燕, 等. 巴戟天多糖含药血清对骨髓间充质干细胞成骨分化过程中Cbfa-1 mRNA表达的影响[J].中国医药科学, 2017, 7(15):27
LAI MX, FENG J, RUAN ZY, et al. Effects of MOP-containing serum on Cbfa-1 mRNA expression during the differentiation from BMSCs to the osteoblast[J].China Med Pharm, 2017, 7(15):27
[10] 沈杰, 马恩耀, 赵志敏, 等. 巴戟天多糖的提取、分离及生物活性研究进展[J].中药新药与临床药理, 2020, 31(2):246
SHEN J, MA EY, ZHAO ZM. Research progress on extraction, separation, and biological activities of polysaccharides from Morindae Officinalis Radix[J].Tradit Chin Drug Res Clin Pharmacol, 2020, 31(2):246
[11] 李鑫洁,李玲玲,鲁艺, 等. 巴戟天寡糖提纯工艺及药理作用研究进展[J].天然产物研究与开发, 2019, 31(2):345
LI XJ, LI LL, LU Y, et al. The research of Morinda officinalis How’s oligosaccharide extraction, purification and pharmacological effects[J].Nat Prod Res Dev, 2019, 31(2):345
[12] 沈佳雯, 金冠钦, 王梦超. 巴戟天属植物中环烯醚萜类化合物研究进展[J].中国药师, 2020, 23(6):1160
SHEN JW, JIN GQ, WANG MC, et al. Research progress in iridoids in plants from Morinda L. [J].China Pharm, 2020, 23(6):1160
[13] ZHANG ZG, ZHANG QY, YANG H, et al. Monotropein isolated from the roots of Morinda officinalis increases osteoblastic bone formation and prevents bone loss in ovariectomized mice[J].Fitoterapia, 2016, 110:166
[14] 中国植物志编辑委员会. 中国植物志[M].北京:科学出版社, 1999:179
Editorial Committee of Flora of China. Flora of China[M].Beijing:Science Press, 1999:179
[15] 郭慧,王谦博,贾力维,等. 中药材DNA条形码技术研究进展[J].中国药师,2016,19(3):566
GUO H, WANG QB, JIA LW, et al. Study progress in DNA barcode of traditional Chinese medicine[J].China Pharm,2016,19(3):566
[16] HEBERT PD, CYWINSKA A, BALL SL. Biological identifications through DNA barcodes[J].Proc Biol Sci, 2003, 270(1512):313
[17] 陈士林,庞晓慧,姚辉,等. 中药DNA条形码鉴定体系及研究方向[J].世界科学技术-中医药现代化,2011,13(5):747
CHEN SL,PANG XH,YAO H,et al. Identification system and perspective for DNA barcoding traditional Chinese materia medica[J].World Sci Technol Mod Tradit Chin Med, 2011, 13(5):747
[18] 陈士林,姚辉,宋经元,等. 基于DNA barcoding(条形码)技术的中药材鉴定[J].世界科学技术-中医药现代化,2007,9(3):7
CHEN SL, YAO H, SONG JY, et al. Use of DNA barcoding to identify Chinese medicinal materials[J].World Sci Technol Mod Tradit Chin Med, 2007, 9(3):7
[19] LI DZ,LIU JQ,CHEN ZD,et al. Plant DNA barcoding in China[J].J Syst Evol,2011, 49(3):165
[20] LI M,CAO H,BUT PPH,et al. Identification of herbal medicinal materials using DNA barcodes[J].J Syst Evol, 2011, 49(3):271
[21] 中国植物志编辑委员会. 中国植物志[M].北京:科学出版社, 1996:264
Editorial Committee of Flora of China. Flora of China[M].Beijing:Science Press,1996:264
[22] 中国中医研究院中药研究所. 中药材品种论述[M].上海:上海科学技术出版社,1964:277
Institute of Chinese Materia Medica China Academy ofChinese Medical Sciences. Dissertateof Traditional Chinese Medicinal Materials Assortment[M].Shanghai:Shanghai Scientific & Technical Publishers, 1964:277
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