目的: 建立金樱根基原之一的粉团蔷薇根质量控制标准。方法: 性状、显微、TLC 法进行定性鉴别; 根据2020 年版《中华人民共和国药典》方法进行检查和浸出物测定;UPLC 测定粉团蔷薇根中构莓苷F1、阿江榄仁亭、野蔷薇苷含量,并以UPLC 建立指纹图谱。结果: 所建立的显微鉴别、薄层色谱法、UPLC 含量测定和指纹图谱方法稳定可行。药材水分、总灰分及浸出物含量：水分不得过13.87%,总灰分不得过8.72%,浸出物不得少于5.08%;11 个批次粉团蔷薇根中所测构莓苷F1、阿江榄仁亭、野蔷薇苷含量范围分别是0.44~12.45、0.32~3.79、1.10~8.08 mg·g-1,暂定本品按干燥品计算,含构莓苷F1 不得少于0.35 mg·g-1, 含阿江榄仁亭不得少于0.26 mg·g-1,含野蔷薇苷不得少于0.88 mg·g-1;同时测定了11 批粉团蔷薇根药材的指纹图谱并建立了共有模式,标定共有峰15 个,指认了其中的构莓苷F1、阿江榄仁亭、野蔷薇苷、蔷薇酸、委陵菜酸5 个成分。结论: 该研究所建方法专属性强,可从整体上对粉团蔷薇根进行鉴别和质量控制,也为最终制定金樱根药材质量标准打下基础。
邓巧玉, 李宇璐, 马国需, 许旭东, 罗轶, 周艳林, 钟小清, 易蔚, 袁经权
. 粉团蔷薇根质量标准的研究*[J]. 药物分析杂志, 2021
, 41(7)
: 1125
-1132
.
DOI: 10.16155/j.0254-1793.2021.07.03
Objective： To establish the quality standard of the roots of Rosa multiflora Thunb. var. cathayensis Rehd. & Wils. Methods： Qualitative identification was carried out by character,microscope and TLC. Examination and extract were determined according to Chinese Pharmacopoeia（ 2020 edition）. The contents of kaji-ichigoside F1,arjunetin and rosamultin were determined by UPLC,and the fingerprints were also established by UPLC. Results： The methods of TLC and UPLC were stable and feasible. The moisture content in the sample should not exceed 13.87%,the total amount of ash should not be more than 8.72%,and the content of extracts should not be less than 5.08%. The contents of kaji-ichigoside F1,arjunetin and rosamultin in 11 batches of samples were 0.44-12.45 mg·g-1,0.32-3.79 mg·g-1 and 1.10-8.08 mg·g-1,respectively. And the minimum contents of the three components should not be less than 0.35 mg·g-1,0.26 mg·g-1 and 0.88 mg·g-1 respectively. The common mode was established after determing the fingerprints of 11 batches from R. multiflora var. cathayensis by UPLC. There were 15 commom peaks in these samples,and five components（ kaji-ichigoside F1,arjunetin,rosamultin, euscaphic acid and tormentic acid） were identified. Conclusion： The methods are specific and can be used to identify and control the quality of the roots of Rosa multiflora Thunb. var. cathayensis Rehd. & Wills in general, which also laid a solid foundation for the final formulation of standards for Radix et Caulis Rosae.
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