目的: 采用高效液相色谱-紫外检测器-蒸发光散射检测器（HPLC-VWD-ELSD）法同时测定黄甲软肝颗粒中没食子酸、葛根素、芍药苷、三七皂苷R1、黄芪甲苷和丹参酮 ⅡA 的含量。方法: 采用Waters Symmetry C18 色谱柱（250 mm×4.6 mm,5 μm）,流动相为甲醇（A）-0.1% 醋酸水溶液（B）,梯度洗脱,流速为1.0 mL·min-1。没食子酸、葛根素和丹参酮 ⅡA 采用VWD 进行检测,检测波长为270 nm;芍药苷,三七皂苷R1 和黄芪甲苷采用ELSD 进行检测,漂移管温度为70 ℃,载气流速2.0 L·min-1。结果: 没食子酸、葛根素、芍药苷、三七皂苷R1、黄芪甲苷和丹参酮 ⅡA 进样量分别在1.444~28.88、2.118~42.36、1.074~21.48、0.218 0~4.360、0.115 1~2.302 和0.066 70~1.334 μg 范围内与峰面积呈良好的线性关系;平均回收率（n=6）分 别为93.8%、98.8%、97.5%、103.0%、100.6% 和96.6%,RSD 分别为1.4%、0.55%、3.3%、4.0%、3.9% 和2.3%。 5 批黄甲软肝颗粒中上述6 个成分的含量测定结果分别为2.612~3.203、9.155~10.342、7.044~9.863、0.622~0.841、1.529~1.697 和0.719~0.957 mg·g-1。结论: 所建立的方法简便、快捷,重复性好,可用于黄甲软肝颗粒中6 个成分的含量测定,为黄甲软肝颗粒全面的质量控制提供科学依据。
王宗权, 黄朝情, 黄志辉, 李艳英, 高进, 黄能听, 刘军, 张保献, 滕利荣
. HPLC-VWD-ELSD 法同时测定黄甲软肝颗粒中6 个成分的含量*[J]. 药物分析杂志, 2021
, 41(7)
: 1148
-1154
.
DOI: 10.16155/j.0254-1793.2021.07.05
Objective： To develop a method of high performance liquid chromatography-variable wavelength detector-evaporative light scattering detection（ HPLC-VWD-ELSD） for simultaneous determination of gallic acid,puerarin,paeoniflorin,notoginsenoside R1,astragaloside and tanshinone ⅡA in Huangjia Ruangan granules. Methods： HPLC assay was performed on a Waters Symmetry-C18 column（ 250 mm×4.6 mm,5 μm） with a mixture of methanol and 0.1% aqueous acetic acid as the mobile phase in gradient elution at the flow rate of 1.0 mL·min-1. Detection wavelength for gallic acid,puerarin and tanshinone ⅡA was set at 270 nm and ELSD was used for detection of paeoniflorin,notoginsenoside R1 and astragaloside. The temperature of the drift tube was maintained at 70 ℃ and the nebulizing gas flow rate was 2.0 L·min-1. Results： The linear ranges of gallic acid, puerarin,paeoniflorin,notoginsenoside R1,astragaloside and tanshinone ⅡA were 1.444-28.88,2.118-42.36, 1.074-21.48,0.218 0-4.360,0.115 1-2.302 and 0.066 70-1.334 μg,respectively. The average recoveries （n=6） were 93.8% with RSD of 1.4%,98.8% with RSD of 0.55%,97.5% with RSD of 3.3%,103.0% with RSD of 4.0%,100.6% with RSD of 3.9%,96.6% with RSD of 2.3%,respectively. The contents of the above-mentioned components in 5 samples were 2.612-3.203,9.155-10.342,7.044-9.863,0.622-0.841,1.529-1.697 and 0.719-0.957 mg·g-1,respectively. Conclusion： The method is simple,rapid and reproducible. It can be used for the determination of the six components in Huangjia Ruangan granules,providing a scientific basis for the comprehensive quality control of Huangjia Ruangan granules.
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