目的: 建立同时测定有柄石韦中1 个二氢黄酮类成分（圣草酚-7-O-β-D- 吡喃葡萄糖醛酸苷）及4 个咖啡酰奎宁酸类成分（绿原酸、4,5- 二咖啡酰奎宁酸甲酯、3,4- 二咖啡酰奎宁酸、1,2- 二咖啡酰- 环戊基-3- 醇）含量的高效液相色谱法。方法: 采用SHIMADZU C18 色谱柱（4.6 mm×250 mm,5 μm）,以乙腈（A）-0.2% 磷酸水溶液（B）为流动相,梯度洗脱,流速1.0 mL·min-1,检测波长为330 nm,柱温30 ℃。结果: 绿原酸、圣草酚-7-O-β-D- 吡喃葡萄糖醛酸苷、4,5- 二咖啡酰奎宁酸甲酯、3,4- 二咖啡酰奎宁酸、1,2- 二咖啡酰- 环戊基-3- 醇进样量分别在0.106 6~2.665 5μg（r=1.000 0）、0.160 2~4.006 0 μg（r=1.000 0）、0.006 5~0.162 5 μg（r=1.000 0）、0.006 9~0.171 5 μg（r=1.000 0）和0.009 6~0.239 0 μg（r=1.000 0）范围内与峰面积呈现良好的线性关系;精密度（RSD ≤ 0.7%）、稳定性（RSD ≤ 0.4%）良好;平均回收率（n=6）分别为101.5%、100.4%、99.7%、97.3% 和101.2%,RSD 分别为0.21%、0.52%、0.57%、0.92% 和1.0%;4 批样品中绿原酸、圣草酚-7-O-β-D- 吡喃葡萄糖醛酸苷、4,5- 二咖啡酰奎宁酸甲酯、3,4- 二咖啡酰奎宁酸和1,2- 二咖啡酰- 环戊基-3- 醇含量范围分别为2.186~7.405、7.694~10.790、0.352~0.511、0.332~0.512 和0.437~0.763 mg·g-1。结论: 该方法简便易行,重复性好,可作为有柄石韦药材的质量控制指标。
Objective： To develop an HPLC method for simultaneous determination of one dihydroflavonoid [（±）-eriodictyol-7-O-β-D-glucuronide] and four caffeoylquinic acids（chlorogenic acid,4, 5-dicaffeoylquinic acid methyl ester,3,4-di-O-caffeoylquinic acid,1,2-O-dicaffeoyl-cychopenta-3-ol） in the leaves of Pyrrosiapetiolosa（ Christ） Ching. Methods： The SHIMADZU C18 column（4.6 mm×250 mm,5 μm） was used,the mobile phase was acetonitrile（A）-0.2% phosphate aqueous（B） with gradient elution,at the flow rate of 1.0 mL·min-1,the detection wavelength was 210 nm,and the column temperature was at 30 ℃ . Results： The linear ranges of chlorogenic acid and（ ±）-eriodictyol-7-O-β-D-glucuronide,4,5-dicaffeoylquinic acid methyl ester,3,4-di-O-caffeoylquinic acid and 1,2-O-dicaffeoyl-cychopenta-3-ol were 0.106 6-2.665 5 μg （r=1.000 0）,0.160 2-4.006 0 μg（r=1.000 0）,0.006 5-0.162 5 μg（r=1.000 0）,0.006 9-0.171 5 μg（r=1.000 0） and 0.009 6-0.239 0 μg（r=1.000 0）,respectively. The precision（RSD ≤ 0.7%） and stability（RSD ≤ 0.4%） were good. The average recoveries（n=6） were 101.5%（RSD=0.21%）,100.4%（RSD=0.52%）,99.7% （RSD=0.57%）,97.3%（RSD=0.92%） and 101.2%（RSD=1.0%）,respectively. The contents of chlorogenic acid, （±）-eriodictyol-7-O-β-D-glucuronide,4,5-dicaffeoylquinic acid methyl ester;3,4-di-O-caffeoylquinic acid and 1,2-O-dicaffeoyl-cychopenta-3-ol in four batches of samples were 2.186-7.405,7.694-10.790,0.352- 0.511,0.332-0.512 and 0.437-0.763 mg·g-1,respectively. Conclusion： This method is specific,accurate and reproducible,and can be used to control the quality of the leaves of P. petiolosa.
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