目的:明确驱虫斑鸠菊药材的基原、鉴别要点以及DNA条形码序列信息。方法:应用体式荧光显微镜、光学显微镜和扫描电镜及其数字化成像技术, 对驱虫斑鸠菊进行性状和显微鉴定研究, 并应用DNA 条形码技术进行分子生物学鉴定研究。结果:驱虫斑鸠菊果实在外形、棱肋、肋间、被毛等性状鉴定特征, 以及石细胞群和纤维群、棱脊处维管束、中果皮内侧“V”字形石细胞带、草酸钙柱晶和方晶等显微鉴定特征上具有明显的鉴别意义。16批驱虫斑鸠菊药材的DNA 条形码鉴定结果一致, 其ITS和ITS2序列在GenBank数据库中均可比对到驱虫斑鸠菊Vernonia anthelmintica, 且一致性为100%。结论:明确了驱虫斑鸠菊药材性状鉴定、显微鉴定的要点, 以及ITS、ITS2序列的信息, 为驱虫斑鸠菊药材的性状鉴定、显微鉴定、DNA条形码鉴定提供依据。
康帅, 刘杰, 房文亮, 唐哲, 乔菲, 连超杰, 戴胜云, 马双成, 郑健
. 维吾尔族习用药材驱虫斑鸠菊的鉴定研究*[J]. 药物分析杂志, 2021
, 41(8)
: 1335
-1342
.
DOI: 10.16155/j.0254-1793.2021.08.06
Objective：To clarify the origin, identification points and DNA barcode sequence information of Fructus Vernoniae. Methods：The macroscopic and microscopic identification of Fructus Vernoniae was studied using stereo microscopy, optical microscopy and scanning electron microscopy combined with digital imaging technology. And the molecular identification of this drug was studied using DNA barcoding technology. Results：The characteristics in appearance, ribs, intercostals, coats, etc of Vernonia anthelmintica showed obvious distinguishing significance in stone cell clusters, fiber clusters, vascular bundles at the ridges, “V”-shaped stone cell belts on the inner side of the mesocarp, calcium oxalate columnar crystals and squares features such as crystal. The DNA barcode identification results of 16 batches of Fructus Vernoniae medicinal materials were consistent, its ITS and ITS2 sequences could be compared to Vernonia anthelmintica in the GenBank database, and the consistency was 100%. Conclusion：The main points of the macroscopic and microscopic identification of Fructus Vernoniae, and the information of ITS, ITS2 sequences, which providing basis for the character identification, microscopic identification, and DNA barcode identification of Fructus Vernoniae is clarified.
[1] 卫生部药品生物制品检定所. 中国民族药志[M]. 北京:人民卫生出版社,1984:312
Institutes for the Control of Pharmaceutical and Biological Products, Ministry of Public Health,PR China. Records of Chinese Ethnic Medicines[M]. Beijing:People’s Medical Publishing House, 1984:312
[2] 中国科学院中国植物志编辑委员会. 中国植物志. 第74 卷 [M]. 北京:科学出版社,1985:8
Editorial Board of Flora of China. Chinese Academy of Sciences Fauna Sinica. Vol 74[M]. Beijing:Science Press,1985:8
[3] YI XL,CUI TT.Identification of the risk HLA-A alleles and autoantigne in han Chinese Vitiligo patients and the association of CD8+T cell reactivity with disease characteristics[J]. Med Sci Monit,2018,24:6489
[4] ANURADHA B,DAVINDER P.Clinical and molecular aspects of Vitiligo treatments[J]. Int J Mol Sci,2018,19:1509
[5] 邓贤兰,陈霞霞,张争光. 井冈山地区18 种唇形科植物果实形状及表面微形态特征研究[J]. 植物研究,2020,40(6):820
DENG XL,CHEN XX,ZHANG ZG.Fruit shape and micromorp-hological characteristics of pericarp surface of 18 Lamiaceae plant in Jinggangshan area[J]. Bull Bot Res,2020,40(6):820
[6] 刘洋,孟爽爽,叶资,等. 4 种溪黄草药材基原植物种子状果实形态特征的比较[J]. 分子植物育种,2021,19(4):1337
LIU Y,MENG SS,YE Z,et al.Comparison of the morphological characteristics of the seed-like fruits of four origins of Xihuangcao[J]. Mol Plant Breed,2021,19(4):1337
[7] 周建理,余晶晶,张琼,等. 中药材微性状鉴别方法的研究[C]. 中华中医药学会第九届中药鉴定学术会议论文集,2008: 202
ZHOU JL,YU JJ,ZHANG Q,et al.Study on the micro-descriptions on Chinese herbal materials[C]. //Proceedings of the 9th Chinese Medicine Appraisal Conference of the Chinese Society of Chinese Medicine,2008:202
[8] 郭慧,王谦博,贾力维,等. 中药材DNA 条形码技术研究进展[J]. 中国药师,2016,19(3):566
GUO H,WANG QB,JIA LW,et al.Study progress in DNA barcode of traditional Chinese medicine[J]. China Pharm,2016,19(3): 566
[9] HEBERT PD,CYWINSKA A,BALL SL.Biological identifications through DNA barcodes[J]. Proc Biol Sci,2003,270(1512): 313
[10] 陈士林,庞晓慧,姚辉,等. 中药DNA 条形码鉴定体系及研究方向[J]. 世界科学技术- 中医药现代化,2011,13(5):747
CHEN SL,PANG XH,YAO H,et al.Identification system and perspective for DNA barcoding traditional Chinese materia medica[J]. World Sci Technol Mod Tradit Chin Med,2011,13(5):747
[11] 陈士林,姚辉,宋经元,等. 基于DNA barcoding(条形码)技术的中药材鉴定[J]. 世界科学技术- 中医药现代化,2007,9(3):7
CHEN SL,YAO H,SONG JY,et al.Use of DNA barcoding to identify Chinese medicinal materials[J]. World Sci Technol Mod Tradit Chin Med,2007,9(3):7
[12] LI DZ,LIU JQ,CHEN ZD,et al.Plant DNA barcoding in China[J]. J Syst Evol,2011,49(3):165
[13] LI M,CAO H,BUT PPH,et al.Identification of herbal medicinal materials using DNA barcodes[J]. J Syst Evol,2011,49(3):271
[14] LIU Y,WANG WQ,CHEN T,et al.New flavonoid glycosides from seeds of Baccharoides anthelmintica[J]. Nat Prod Res,2020,34(2):284
[15] 中国科学院中国植物志编辑委员会. 中国植物志[M/OL]. 北京:科学出版社,1985[2021-06-09]. http://www.iplant.cn/info/ Baccharoides%20anthelmintica?t=z
Editorial Board of Flora of China. Chinese Academy of Sciences Fauna Sinica.[M/OL]. Beijing:Science Press,1985[2021-06-09]. http://www.iplant.cn/info/Baccharoides%20anthelmintica?t=z
[16] 曾献存,刘星,谢雪倩,等. 驱虫斑鸠菊果实的生药学鉴定研究[J]. 时珍国医国药,2020,31(9):2160
ZENG XC,LIU X,XIE XQ,et al.Pharmacognostic identification of Vernonia anthelminticus fruits[J]. Lishizhen Med Mater Med Res, 2020,31(9):2160
[17] 张英涛,艾铁民. 驱虫斑鸠菊果实的显微鉴别研究[J]. 中草药, 2000,31(9):707
ZHANG YT,AI TM.Study on the microscopic identification of Vernonia anthelminticus fruits[J]. Chin Tradit Herb Drugs,2000, 31(9):707
