目的:建立超高效液相色谱(UPLC)法同时测定牛樟芝子实体中樟芝酸(antcin)K、C、H、B、A, 去氢硫色多孔菌酸和去氢齿孔酸7 个三萜类成分的含量。方法:采用ACQUITY UPLC BEH C18 色谱柱 (100 mm×2.1 mm,1.7 μm),以乙腈-0.2% 甲酸水溶液为流动相,梯度洗脱,流速0.3 mL·min-1,检测波长254 nm, 柱温为30 ℃。结果:樟芝酸K、樟芝酸C、樟芝酸H、樟芝酸B、樟芝酸A、去氢硫色多孔菌酸和去氢齿孔酸在一定浓度范围内呈良好的线性关系(r=0.999 5~0.999 8),平均回收率(n=6)分别为97.9%、98.2%、97.2%、97.8%、98.3%、98.1% 及97.5%(RSD<2.0%)。方法的精密度RSD 均小于1.5%,重复性RSD 小于2.0%,供试品溶液放置48 h 内稳定。18 批样品的测定结果表明,牛樟芝子实体中,麦角甾烷型三萜的含量较高,占7.95%~14.91%;羊毛脂烷型三萜的含量较少,仅占0.36%~1.08%。结论:经方法学验证,本法可用于牛樟芝三萜类成分的质量控制。
Objective: To develop an UPLC method for simultaneous determination of seven triterpenoids:antcin K,antcin C,antcin H,antcin B,antcin A,dehydrosulphurenic acid(DSA),and dehydroeburicoic acid(DEA) in Antrodia cinnamomea fruiting bodies. Methods: The ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm) was adopted,the mobile phase was acetonitrile-0.2% formic acid aqueous solution,with a gradient elution. The flow rate was 0.3 mL·min-1,the detection wavelength was set at 254 nm,and the column temperature was set at 30 ℃ . Results: Antcin K,antcin C,antcin H,antcin B,antcin A,DSA,and DEA exhibited good linear correlation in the given concentration ranges(r=0.999 5-0.999 8). The average recoveries(n=6) were 97.9%,98.2%,97.2%,97.8%,98.3%, 98.1%,and 97.5% respectively(RSD<2.0%). The RSDs of precision were less than 1.5% for the seven components, and the RSDs of reproducibility were less than 2.0%. The solution was stable within 48 h. The determination results of 18 batches of samples showed that the contents of ergostanes were high(7.95%-14.91%) in Antrodia cinnamomea fruiting bodies,while the lanostanes were relatively low(0.36%-1.08%). Conclusion: This method is proved by methodology validation that it can be used for determination of triterpenoids in Antrodia cinnamomea.
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