成分分析

UPLC法同时测定牛樟芝子实体中7个三萜类成分的含量

展开
  • 1.福建省食品药品质量检验研究院,福州 350001;
    2.福建省医科大学药学院,福州 350108;
    3.联勤保障部队第九〇九医院/厦门大学附属东南医院药剂科,漳州 363000
第一作者 Tel:(0591)87521752;E-mail:mumulql@163.com
*Tel:(0591)87513886;E-mail:517653339@qq.com

修回日期: 2021-05-27

  网络出版日期: 2024-07-15

Simultaneous determination of seven triterpenoids in Antrodia cinnamomea fruiting bodies by UPLC

Expand
  • 1. Fujian Institute for Food and Drug Quality Control,Fuzhou 350001,China;
    2. School of Pharmacy,Fujian Medical University,Fuzhou 350108,China;
    3. The Pharmacy Department, No.909 Hospital of PLA/Southeast Hospital Affiliated to Xiamen University,Zhangzhou,363000,China

Revised date: 2021-05-27

  Online published: 2024-07-15

摘要

目的:建立超高效液相色谱(UPLC)法同时测定牛樟芝子实体中樟芝酸(antcin)K、C、H、B、A, 去氢硫色多孔菌酸和去氢齿孔酸7 个三萜类成分的含量。方法:采用ACQUITY UPLC BEH C18 色谱柱 (100 mm×2.1 mm,1.7 μm),以乙腈-0.2% 甲酸水溶液为流动相,梯度洗脱,流速0.3 mL·min-1,检测波长254 nm, 柱温为30 ℃。结果:樟芝酸K、樟芝酸C、樟芝酸H、樟芝酸B、樟芝酸A、去氢硫色多孔菌酸和去氢齿孔酸在一定浓度范围内呈良好的线性关系(r=0.999 5~0.999 8),平均回收率(n=6)分别为97.9%、98.2%、97.2%、97.8%、98.3%、98.1% 及97.5%(RSD<2.0%)。方法的精密度RSD 均小于1.5%,重复性RSD 小于2.0%,供试品溶液放置48 h 内稳定。18 批样品的测定结果表明,牛樟芝子实体中,麦角甾烷型三萜的含量较高,占7.95%~14.91%;羊毛脂烷型三萜的含量较少,仅占0.36%~1.08%。结论:经方法学验证,本法可用于牛樟芝三萜类成分的质量控制。

本文引用格式

李沁, 叶财发, 游勇基 . UPLC法同时测定牛樟芝子实体中7个三萜类成分的含量[J]. 药物分析杂志, 2021 , 41(9) : 1576 -1582 . DOI: 10.16155/j.0254-1793.2021.09.10

Abstract

Objective: To develop an UPLC method for simultaneous determination of seven triterpenoids:antcin K,antcin C,antcin H,antcin B,antcin A,dehydrosulphurenic acid(DSA),and dehydroeburicoic acid(DEA) in Antrodia cinnamomea fruiting bodies. Methods: The ACQUITY UPLC BEH C18 column(100 mm×2.1 mm,1.7 μm) was adopted,the mobile phase was acetonitrile-0.2% formic acid aqueous solution,with a gradient elution. The flow rate was 0.3 mL·min-1,the detection wavelength was set at 254 nm,and the column temperature was set at 30 ℃ . Results: Antcin K,antcin C,antcin H,antcin B,antcin A,DSA,and DEA exhibited good linear correlation in the given concentration ranges(r=0.999 5-0.999 8). The average recoveries(n=6) were 97.9%,98.2%,97.2%,97.8%,98.3%, 98.1%,and 97.5% respectively(RSD<2.0%). The RSDs of precision were less than 1.5% for the seven components, and the RSDs of reproducibility were less than 2.0%. The solution was stable within 48 h. The determination results of 18 batches of samples showed that the contents of ergostanes were high(7.95%-14.91%) in Antrodia cinnamomea fruiting bodies,while the lanostanes were relatively low(0.36%-1.08%). Conclusion: This method is proved by methodology validation that it can be used for determination of triterpenoids in Antrodia cinnamomea.

参考文献

[1] 梁蕾,向婷,李红保,等.牛樟芝及其药理功能[J]. 中药材,2019, 42(1):239
LIANG L,XIANG T,LI HB,et al.Antrodia cinnamomea and its pharmacological function[J]. J Chin Med Mat,2019,42(1):239
[2] 张远腾,李晓波.牛樟芝化学成分及其药理作用研究进展[J]. 中草药,2016,47(6):1034
ZHANG YT,LI XB.Research progress on active components of Antrodia cinnamomea and their pharmacological effects[J]. Chin Tradit Herb Drugs,2016,47(6):1034
[3] CHANG JS,KUO HP,CHANG KL,et al.Apoptosis of hepatocellular carcinoma cells induced by nanoencapsulated polysaccharides extracted from Antrodia camphorata[J]. PLos One, 2015,10(9):e0136782
[4] LEE TH,CHEN CC,CHEN JJ,et al.New and cytotoxic components from Antrodia camphorata[J]. Molecules,2014,19(12):21379
[5] LU MK,LIN TY,CHAO CH,et al.Molecular mechanism of Antrodia cinnamomea sulfated polysaccharide on the suppression of lung cancer cell growth and migration via induction of transforming growth factor β receptor degradation[J]. Int J Biol Macromol, 2017,95:1144
[6] CHEN YF,WANG SH,CHANG SJ,et al.Zhankuic acid A as a novel JAK2 inhibitor for the treatment of concanavalin A induced hepatitis[J]. Biochem Pharmacol,2014,91(2):217
[7] GOKILA-VANI M,KUMAR KJS,LIAO JW,et al.Antcin C from Antrodia cinnamomea protects liver cells against free radical-induced oxidatives stress and apotosis in vitro and in vivo through Nrf2-dependent mechanism[J]. Evid-Based Compl Alternat Med, 2013,2013:296082
[8] LIN CC,PAN IH,LI YR,et al.The adjuvant effects of high-molecule-weight polysaccharides purified from Antrodia cinnamomea on dendritic cell function and DNA vaccines[J]. Plos One,2015, 10(2):e0116191
[9] QIAO X,WANG Q,JI S,et al.Metabolites identification and multi-component pharmacokinetics of ergostane and lanostane triterpenoids in the anticancer mushuroom Antrodia cinnamomea[J]. J Pharm Biomed Anal,2015,111:266
[10] DU YC,WU TY,CHANG FR,et al.Chemical profiling of the cytotoxic triterpenoids-concentrating fraction and characterization of stereo-isomer ingredients from Antrodia camphorata.[J]. J Pharm Biomed Anal,2012,58:182
[11] 陈菲,张奉苏,刘训红,等.樟芝菌粉质量的初步分析[J]. 中药材,2012,35(10):239
CHEN F,ZHANG FS,LIU XH,et al.Preliminary Analysis of Quality of Antrodia camphorata powder[J]. J Chin Med Mater, 2012,35(10):239
[12] 陆震鸣,陶文沂,许泓瑜,等.樟芝菌粉三萜类化合物含量的测定[J]. 中成药,2008,30(3):402
LU ZM,TAO WY,XU HY,et al.Quantitative analysis of triterpenoids from Antrodia camphorata in submerged culture[J]. Chin Tradit Pat Med,30(3):402
[13] 张寅,许泓瑜,陆震鸣,等.薄层色谱-分光光度法测定樟芝菌粉中的总三萜含量[J]. 天然产物研究与开发,2010,22(4):639
ZHANG Y,XU H Y,LU ZM,et al.Determination of total triterpenoids in Antrodia camphorata in submerged culture by TLC-spectrophotometry method[J]. Nat Prod Res Dev,2010,22(4):639
[14] 杨彬君,吴建国,吴岩斌,等.紫外分光光度法测定牛樟芝子实体的总三萜含量[J]. 福建中医药,2018,49(4):70
YANG BJ,WU JG,WU YB,et al.Determination of total triterpenoids in the Antrodia cinnamomea fruiting bodies by UV spectrophotometry[J]. Fujian J Tradit Chin Med,2018,49(4):70
[15] HUANG HS,HSU JL,YU CC,et al.Qualitative and quantitative analysis of seven signature components in the fruiting body of Antrodia cinnamomea by HPLC-ESI-MS/MS[J]. Acta Chromatogr, 2016,28:387
[16] QIAO X,SONG W,WANG Q,et al.Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea[J]. RSC Adv,2015,5:47040
文章导航

/