目的:基于HPLC 指纹图谱与化学模式识别相结合的方法,比较3 种木通指纹图谱,为其进一步开发利用及合理使用提供依据。方法:收集不同产地五叶木通(Akebia quinata(Thunb.)Decne.)、三叶木通(Akebia trifoLiata(Thunb.)Koidz)、白木通(Akebia trifoliata(Thunb.)Koidz.van australis(Diels)Rehd.)27 批,采用“中药色谱指纹图谱相似度评价系统”(2012 版)软件建立木通药材的HPLC 指纹图谱并进行相似度评价,使用 SPSS 23.0、SIMCA 14.1 软件进行主成分分析(PCA)和偏最小二乘法分析(PLS-DA)。结果:建立了适用于3 种不同木通药材的指纹图谱,确定7 个共有峰,23 个特征差异峰。27 批样品的相似度在0.837~0.995 之间。经PCA 和PLS-DA,木通药材样品按来源明显聚集为3 组,3 组样品间差异标志成分共有3 个。结论:建立的方法可有效区分3 种不同来源木通药材,为木通药材的质量控制、质量评价和鉴定提供了提供了参考。
Objective: Based on the combination of HPLC fingerprint and chemical pattern recognition,to compare the fingerprint of three species of akebiaquinata,and to provide the basis for its further development and utilization and rational use. Methods: 27 batches of Akebia quinata(Thunb.)Decne,Akebia trifoLiata(Thunb.) Koidz and Akebia trifoLiata(Thunb.)Koidz. Van Australis(Diels)Rehd. The HPLC fingerprints of 27 batches of akebiaquinata were established and the similarity evaluation was conducted by using the software of“ Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System”(2012 version). The principal component analysis(PCA) and partial least square discrimination analysis(PLS-DA) were performed by SPSS 23.0 and SIMCA 14.1 software. Results: 7 common peaks and 23 characteristic peaks were identified by establishing the fingerprint for three different akebiaquinata. The similarities of 27 batches of samples ranged from 0.837 to 0.995. According to PCA and PLS-DA,the samples were obviously clustered into 3 groups according to their origin,and 3 components were identified as the distinguishing markers. Conclusion: The established method can effectively distinguish the three kinds of akebiaquinata from different sources,and provides a reference for quality control, quality evaluation and identification of akebiaquinata.
[1] 中华人民共和国药典2020 年版. 一部[S]. 2020:65
Chp 2020.Vol Ⅰ[S]. 2020:65
[2] 黄和平,黄璐琦,王键,等. 木通基源考证、药用沿革与资源[J]. 中成药,2013,35(11):2488
HUANG HP,HUANG LQ,WANG J,et al.Textual research on the basic source,medicinal history and resources of Akebia Dence[J]. Chin Tradit Pat Med,2013,35(11):2488
[3] 刘岩庭,侯雄军,谢月,等. 木通属植物化学成分及药理作用研究进展[J]. 江西中医学院学报,2012,24(4):87
LIU YT,HOU XJ,XIE Y,et al.Overview of research on chemical constituents and pharmacological activities of Akebia Dence[J]. J Jiangxi Univ Tradit Chin Med,2012,24(4):87
[4] 高慧敏,王智民. 木通属药用植物研究进展[J]. 中国中药杂志, 2006,31(1):10
GAO HM,WANG ZM.Overview of research on medicinal plants of Akebia Dence[J]. China J Chin Mater Med,2006,31(1):10
[5] 刘桂艳,郑健,余振喜,等. 五叶木通藤茎甾体和三萜成分研究[J]. 中药材,2005,28(12):1060
LIU GY,ZHENG J,YU ZX,et al.Studies on steranes and triterpenoids from the stems of Akebia quinata Decne[J]. J Chin Med Mater,2005,28(12):1060
[6] 袁铭铭,王茜,吴西,等. 三叶木通正丁醇部位化学成分分离鉴定[J]. 中国实验方剂学杂志,2020,26(1):139
YUAN MM,WANG Q,WU X,et al.Chemical constituents of n-butanol extract part of Akebia trifoliata Caulis[J]. Chin J Exp Tradit Med Form,2020,26(1):139
[7] 郭林新,马养民,李梦云,等. 三叶木通利尿活性部位筛选及其化学成分[J]. 中国实验方剂学杂志,2017,23(3):66
GUO LX,MA YM,LI MY,et al.screening of diuretic activity fractions and chemical compositions in rattan of Akebia trifoliate[J]. Chin J Exp Tradit Med Form,2017,23(3):66
[8] 柳岳超,李洪亮,曾小华,等. 三叶木通藤茎的化学成分和药理作用研究进展[J]. 上海中医药大学学报,2020,34(3):99
LIU YC,LI HL,ZENG XH,et al.Research progress on chemical components and pharmacological effects of Akebia trifoliata (Thunb.) koidz.stem[J]. J Shanghai Univ Tradit Chin Med,2020,34(3):99
[9] 孙莉华,安儒峰,庄文选. 关木通的肾毒性及其防护[J]. 中药材,2002,25(5):369
SUN LH,AN RF,ZHUANG WX.Renal toxicity and its protection of Aristolochia Manshuriensis[J]. J Chin Med Mater,2002,25(5):369
[10] 裘奇,刘志红,陈惠萍,等. 木通所致大鼠急性肾损伤的实验观察[J]. 肾脏病与透析肾移植杂志,1999,8(1):3
QIU Q,LIU ZH,CHEN HP,et al.Chinese herb nephropathy: experience of three cases of nephrotoxicity caused by Akebia quinata[J]. Chin J Nephrology,Dial Transplant,1999,8(1):3
[11] 黄得栋,晋玲,林丽,等. 木通及川木通流通使用情况调查分析[J]. 中国中医药信息杂志,2017,24(3):1
HUANG DD,JIN L,LIN L,et al.Investigation of Akebiae Caulis and Clematidis Armandii Caulis[J]. Chin J Inf Tradit Chi Med,2017, 24(3):1
[12] 郭隆钢,金红宇,张奕尧,等. 灵芝和紫芝对照提取物在灵芝样品指纹图谱分析的应用[J]. 中国药学杂志,2020,55(5):349
GUO LG,JIN HY,ZHANG YY,et al.Application of Ganoderma lucidum and Ganoderma sinense extract reference substance in the fingerprint analysis of Ganoderma[J]. Chin Pharm J,2020,55(5):349
[13] 袁静,戴文科,李京华.HPLC 指纹图谱技术结合PLS-DA 在辛芩颗粒质量控制中的应用[J]. 药物分析杂志,2020,40(2):304
YUAN J,DAI WK,LI JH.Application of HPLC fingerprint and PLS-DA in quality control of Xinqin granules[J]. Chin J Pharm Anal,2020,40(2):304
[14] 杨红燕,项微微,李玲燕.HPLC 法指纹图谱在山慈菇真伪鉴别中的应用[J]. 浙江中医杂志,2020,55(1):72
YANG HY,XIANG WW,LI LY.Application of HPLC fingerprint in the identification of Cymbidium cymbidis[J]. Zhejiang J Tradit Chin Med,2020,55(1):72
[15] 李颖硕,张晓栋,杨楠楠,等. 指纹图谱结合一测多评模式在前胡质量评价中的应用[J]. 时珍国医国药,2019,30(7):1557
LI YS,ZHANG XD,YANG NN,et al.Application of fingerprint combined with one test and multiple evaluation mode in quality evaluation of Qianhu[J]. Lishizhen Med Mater Med Res,2019,30(7):1557
