标准研讨

不同加工方式的渝产天麻指纹图谱及化学成分含量分析*

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  • 重庆市中药研究院,重庆市中药药学评价工程技术研究中心,重庆 400065
第一作者 Tel:15756391778;E-mail:churui2020007@163.com
**Tel:(023)89029025;E-mail:lina1281@163.com

收稿日期: 2020-11-16

  网络出版日期: 2024-07-15

基金资助

*重庆市技术创新与应用示范专项社会民生类重点研发项目(csts2018jscx-mszdX0058); 重庆市现代山地特色高效农业产业技术体系创新团队建设项目(2020 年); 重庆市中药研究院基本科研业务费项目(2019 年)

PLC fingerprint and chemical composition analysis of Gastrodia elata produced in Chongqing by different processing method*

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  • Chongqing Academy of Chinese Traditional Materia Medica,Chongqing Engineering Research Center of Pharmaceutical Evaluation of Chinese Medicine,Chongqing 400065,China

Received date: 2020-11-16

  Online published: 2024-07-15

摘要

目的:建立渝产天麻的HPLC 指纹图谱及多成分含量测定方法,研究不同加工方式的渝产天麻HPLC 指纹图谱以及化学成分含量差别,并评估不同加工方式对其活性成分含量变化的影响。方法:采用高效液相色谱(HPLC)法将28 份渝产天麻样品经过60% 甲醇提取后,以乙腈-0.1 % 磷酸水为流动相进行梯度洗脱,检测波长为220 nm,流速为1.0 mL·min-1,柱温25 ℃ ,进行测定;通过直观分析、PCA 以及OPLS-DA 聚类分析,比较不同加工组别渝产天麻的指纹图谱及多成分含量差异。结果:4 组天麻样品组内对比,HPLC 指纹图谱与共有模式之间相似度较高;不同加工组别其指纹图谱特征和成分含量存在明显差异:天麻生品经过加工后天麻素及巴利森苷类活性成分含量明显升高;PCA 及OPLS-DA 模式下分析显示, 天麻生品与饮片(药材)的化学成分差异较大,闪爆处理后所得天麻样品间差异较小,特别是闪爆强度大的生品与蒸制后闪爆的天麻在化学成分构成上相近。结论:所建立的指纹图谱方法精密度高,重复性好,稳定可靠;对比渝产天麻生品,经过传统炮制或者蒸汽闪爆加工后,其活性成分含量大幅度增加,药用价值更高。相比较于传统的炮制方法,蒸汽闪爆操作简单,耗时少,是1 种新型的、有前景的中药材预处理方式。

本文引用格式

储瑞, 励娜, 陈一龙, 李胜容, 蒋振华, 王贤英, 何磊磊 . 不同加工方式的渝产天麻指纹图谱及化学成分含量分析*[J]. 药物分析杂志, 2021 , 41(9) : 1621 -1633 . DOI: 10.16155/j.0254-1793.2021.09.16

Abstract

Objective: To establish the HPLC fingerprint and multi-component determination method for Gastrodia elata produced in Chongqing,to investigate the variation in HPLC fingerprints and chemical contents between Gastrodia elata with different pretreatment methods,and to evaluate the influence of different pretreatment methods on the variation of the contents. Methods: 28 different samples of Gastrodia elata were extracted with 60% phase. The detection wavelength was 220 nm,the flow rate was 1.0 mL·min-1 and the column temperature was 25 ℃ . Through visual analysis,PCA and OPLS-DA analysis,fingerprints and chemical contents between Gastrodia elata in different pretreatment groups were compared. Results: Resultsof intra-group comparison indicated the fingerprints of samples in 4 groups were similar to their own common pattern. But obvious deferences were found in fingerprints and contents among different groups. The content of gastrodin and parishin significantly increased after processing. By PCA and OPLS-DA pattern recognition,the chemical composition in the raw and decoction pieces(crude drugs)was quite different. The difference of gastrodia elata samples obtained after steam explosion treatment was small. Especially,the raw products with high steam explosion intensity was similar to the steamed products before steam explosion. Conclusion: The established fingerprints were precise,repeatable,stable and reliable. Compared with fresh gastrodia elata,the contents of active ingredients and the medicinal value greatly increased after the steam flash explosion treatment. Compared with the traditional processing method,the steam explosion was simple and time-consuming,and it is a new and promising processing method for TCM raw materials.

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