目的：建立HPLC 法同时测定荨麻疹丸（赤芍、防风、当归、黄芩、白芷、何首乌）中芍药苷、升麻素苷、阿魏酸、5-O-甲基维斯阿米醇苷、黄芩苷、欧前胡素、异欧前胡素、大黄素、大黄素甲醚的含量。方法：采用高效液相色谱法。色谱柱为JADE-PAK ODS-AQ C18,流动相为甲醇-0.2% 磷酸水溶液,梯度洗脱,流速1.0 mL·min-1,检测波长230 nm（芍药苷）、254 nm（升麻素苷、5-O-甲基维斯阿米醇苷、大黄素、大黄素甲醚）、280 nm（黄芩苷）、300 nm（欧前胡素、异欧前胡素）、321 nm（阿魏酸）,柱温25 ℃,进样量10 μL。结果：9 个成分在各自范围内线性关系良好（r≥0.999）,平均加样回收率为95.2%~101.0%,RSD 为1.2%~2.0%。3批样品中芍药苷、升麻素苷、阿魏酸、5-O-甲基维斯阿米醇苷、黄芩苷、欧前胡素、异欧前胡素、大黄素、大黄素甲醚含量范围分别为2.014~2.161、0.205~0.220、0.032~0.038、0.241~0.251、0.048~0.050、0.785~0.811、0.042~0.049、0.095~0.102、0.037~0.041 mg·g-1。结论：该方法准确,重复性好,可用于荨麻疹丸的质量控制。
Objective: To establish a method for the simultaneous determination of paeoniflorin,prim-Oglucosylcimifugin, ferulic acid,5-O-methylvisammioside,baicalin,imperatorin,isoimperatorin,emodin and physcion in urticaria pills. Methods: HPLC was performed on the column of JADE-PAK ODS-AQ C18with the mobile phase of methanol-phosphoric acid at a flow rate of 1. 0 mL·min-1. The detection wavelength was 230 nm for paeoniflorin,254 nm for cimifugin,5-O-methylvisammioside,emodin and parietin,280 nm for baicalin and 300 nm for imperatorin and isoimperatorin,321 nm for ferulic acid,respectively. Column temperature was 25 ℃, and the volume injection was 10 μL. Results: Nine constituents showed good linear relationships within their own ranges（r≥0. 999）, and the average recoveries were 95. 2%-101. 0% with the RSDs of 1. 2%-2. 0%. The content ranges of paeoniflorin,prim-O-glucosylcimifugin,ferulic acid, 5-O-methylvisammioside, baicalin, imperatorin, isoimperatorin, emodin, physcion in 3 samples were 2. 014-2. 161,0. 205-0. 220, 0. 032-0. 038, 0. 241-0. 251, 0. 048-0. 050, 0. 785-0. 811,0. 042-0. 049,0. 095-0. 102,0. 037-0. 041 mg·g-1, respectively. Conclusion: This simple, accurate and reproducible method can be used for the quality control of urticaria pills.
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