综述与专论

基于微流控芯片技术的秀丽隐杆线虫 药物筛选体系研究进展*

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  • 1.上海交通大学 医学院 公共卫生学院 虹桥国际医学研究院,上海 200336;
    2.上海交通大学 微纳电子学系 微米 / 纳米加工技术国家级重点实验室,上海 200240
第一作者 Tel:15921179957; E-mail:wujiaying1996@sjtu.edu.cn
** 栾 洋 Tel:18930382127;E-mail:yluan@sjtu.edu.cn;
陈 翔 Tel:13764260145;E-mail:xiangchen@sjtu.edu.cn

收稿日期: 2020-11-02

  网络出版日期: 2024-07-15

基金资助

* 上海交通大学医工交叉重点项目(ZH2018ZDA22)

Advances in drug screening of C. elegans based on microfluidic chip techniques*

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  • 1. Hongqiao International Institute of Medicine,Faculty of Public Health,School of Medicine,Shanghai Jiao Tong University, Shanghai 200336,China;
    2. National Key Laboratory of Science and Technology on Micro/Nano Fabrication,Department of Micro/Nano Electronics,Shanghai Jiao Tong University,Shanghai 200240,China

Received date: 2020-11-02

  Online published: 2024-07-15

摘要

活性化合物筛选和早期药物毒性筛选是创新药物研究的起点,整合新技术的高通量筛选成为研究 热点。秀丽隐杆线虫作为一种常用的体内模式生物,既可以模拟化合物在体内的代谢变化,又可以实现药 物的高通量筛选。微流控芯片通道与线虫大小尺度相匹配,能实现线虫在芯片内分选、固定、长期培养、 自动化给药、高分辨率成像和图像自动分析等,为基于线虫的高通量高内涵药物毒性筛选研究提供了良 好的平台。本文对近年来用于药物线虫毒性测试的微流控技术进行综述,并针对此领域的未来发展提出 展望。

本文引用格式

吴佳颖, 李栋基, 陈翔, 栾洋 . 基于微流控芯片技术的秀丽隐杆线虫 药物筛选体系研究进展*[J]. 药物分析杂志, 2021 , 41(6) : 929 -942 . DOI: 10.16155/j.0254-1793.2021.06.01

Abstract

Screening of active compounds and early-stage drug toxicity screening are the basement of innovative drug researches,and high-throughput drug screening integrated with new technologies has become a popular research topic. As a powerful in vivo alternative assay system,the nematode Caenorhabditis elegansC. elegans) can not only simulate the dynamic changes of compounds in the body but also realize high-throughput exposure of drugs. Microfluidic chip is integrated with the nematodes due to its suitable size and the advantage of low reagent consumption. It can realize the following functions:sorting,immobilization,long-term cultivation,automated drug delivery,high-resolution imaging and automatic image analysis,which provides a good platform for high- throughput and high-content drug screening researches based on C. elegans. In this paper,we reviewed the microfluidic chip techniques for drug toxicology studies based on nematodes in recent years,and the future research directions were proposed.

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