目的: 建立药品中基因毒性杂质苯残留量的检测方法,为药物分析中苯的检测提供方法依据。方法: 采用 ZB-50 毛细管柱（30 m× 0.53 mm× 1.0 μm）,柱温为程序升温（起始温度 40 ℃,保持 1 min,以 25 ℃· min-1 的速率升温至 180 ℃,保持 3 min）;进样口 200 ℃,氢火焰离子化检测器（FID）温度为:250 ℃。载 气为氮气,流速 2.0 mL·min-1,分流比为 10∶1;顶空进样,顶空平衡温度 90 ℃,平衡时间为 20 min。结果: 苯浓度范围在 0.034~0.407 µg·mL-1 线性关系良好（r=0.999 5）,平均加样回收率（n=9）为 92.3%（RSD= 2.4%）,检测下限为 11.0 ng·mL-1,定量下限为 34.0 ng·mL-1,5 批样品中均未检出苯。结论: 所建立的方法能够较好地对药品中残留的苯进行限度测定,极大的提高苯的检测效率。
[1] 刘志伟,陈思,毛佳伟,等.罐采样/气相色谱-质谱法测定页岩气中的苯系物含量[J].四川化工,2020(1):38
LIU ZW,CEHN S,MAO JW,et al.Determination of benzene series in shale gas by tank sampling/gas chromatography-mass spectrometry[J].Sichuan Chem Ind,2020(1):38
[2] 潜森芝,何灵燕,周华强.乙酸乙烯酯中痕量苯分析方法的建立[J].石油化工技术与经济,2020,36(1):28
QIAN SZ,HE LY,ZHOU HQ.Determination of trace benzene in vinyl acetate[J].Petrochem Technol Econ,2020,36(1):28
[3] 王文佳,吴凡,马小东.一种同时检测工业己烷中正己烷和苯等12 种化合物的方法[J].石化技术,2020,27(12):3,15
WANG WJ,WU F,MA XD.A method for simultaneous determination of n-hexane and benzene in industrial hexane[J]. Petrochem Technol,2020,27(12):3,15
[4] 李中贤,刘小培,王俊伟,等.二次热解吸-气相色谱法测定工作场所空气中苯系物[J].河南科学,2018,36(1):54
LI ZX,LIU XP,WANG JW,et al.Determination of benzene series in the air of workplace by secondary thermal desorption gas chromatography[J].Henan Sci,2018,36(1):54
[5] 刘祥云,龙剑英.吹扫捕集/气相色谱-质谱法测定土壤中的苯系物[J].环境与发展,2019,31(1):97
LIU XY,LONG JY.Determination of benzene series in soil by Purge and trap/GC-MS[J].Environ Devel,2019,31(1):97
[6] 周建科,韩康,张前莉,等.顶空单液滴微萃取-气相色谱法检测马来酸氯苯那敏中苯系物残留量[J].药物分析杂志,2006,26(10):1480
ZHOU JK,HAN K,ZHANG LL,et al.Determination of benzene residues in chlorphenamine maleate by headspace single drop microextraction and gas chromatography[J].Chin J Pharm Anal, 2006,26(10):1480
[7] 姜承志,李媛,李笑冉.活性炭吸附-红外光谱法定性分析空气中的苯[J].辽宁化工,2020,49(12):1581
JIANG CZ,LI Y,LI XR.Qualitative analysis of benzene in air by activated carbon adsorption infrared spectroscopy[J].Liaoning Chem Ind,2020,49(12):1581
[8] 闫建康. 二维气相色谱技术在汽油含氧化合物和苯分析中的应用[J].石油与天然气化工,2020,49(6):101
YAN JK.Application of two dimensional gas chromatography in the analysis of oxygenated compounds and benzene in gasoline[J]. Petrol Nat Gas Chem Ind,2020,49(6):101
[9] 史惠娟. 气相色谱法检测煤气中苯含量[J].中国石油和化工标准与质量,2019(23):54
SHI HJ.Determination of benzene in gas by gas chromatography[J]. China Petrol Chem Stand Qual,2019(23):54
[10] 栾琳,赵霞,孙会敏.胶囊壳油墨印字中苯类溶剂残留检测[J]. 药物分析杂志,2012,32(8):1325
LUAN L,ZHAO X,SUN HM.Detection of benzene solvent residue in printing ink of capsule shell[J].Chin J Pharm Anal,2012,32(8):1325
[11] 张文生,宋晓涛,武珊珊.气相色谱法测定提取物中苯、甲苯、间二甲苯、对二甲苯、邻二甲苯和苯乙烯的残留量[J].药物分析杂志,2006,26(3):393
ZHANG WS,SONG XT,WU SS.Determination of benzene, toluene,m-xylene,p-xylene,o-xylene and styrene in the extract by gas chromatography[J].Chin J Pharm Anal,2006,26(3):393
[12] 杨向龙,张衡.气相色谱法快速测定三聚甲醛中甲缩醛、甲酸甲酯、甲醇、苯、二氧戊环含量[J].宁夏工程技术,2020(1):36 YANG XL,ZHANG H.Rapid determination of methylal,methyl formate,methanol,benzene and dioxolane in trioxane by gas chromatography[J].Ningxia Eng Technol,2020(1):36
;[13] 李水英,吴长华,钟晶.气相色谱法测定工业己烷中苯含量[J]. 企业技术开发,2019,38(2):12
LI CY,WU CH,ZHONG J.Determination of benzene in industrial hexane by gas chromatography[J].Technol Deve Enterp,2019,38(2):12
[14] 韩陈,孙多志,左莹,等.顶空-气相色谱法测定食品接触材料中14 种苯类化合物[J].食品工业,2019(4):76
HAN C,SUN DZ,ZUO Y,et al.Determination of 14 benzene compounds in food contact materials by headspace gas chromatography[J].Food Ind,2019(4):76
[15] 江丰,朱正伟,王会霞,等.食用植物油中苯残留量的测定[J]. 食品与发酵科技,2020,56(5):118,127
JIANG F,ZHU ZW,WANG HX,et al.Determination of benzene residue in edible vegetable oil[J].Food Ferment Technol.Determination of benzene residue in edible vegetable oil[J].Food Ferment Technol,2020, 56(5):118,127
[16] 曾茵,刘云贵,朱绍严,等.气相色谱法测定云石胶溶剂中苯、甲苯 + 二甲苯含量[J].贵州科学,2020,38(5):89
ZENG Y,LIU YG,ZHU SY,et al.Determination of benzene,toluene+ xylene in dolomite glue solvent by gas chromatography[J].Guizhou Sci,2020,38(5):89
