目的: 建立银黄口服液中 7 个成分（新绿原酸、绿原酸、隐绿原酸、3,4-O- 二咖啡酰奎宁酸、3,5-O- 二咖啡酰奎宁酸、4,5-O- 二咖啡酰奎宁酸、黄芩苷）的高效液相色谱方法,考察双标线性校正法用于色谱 峰定性的可行性,并比较双标线性校正法和相对保留时间法 2 种替代对照品定性方法的优劣。方法: 采用25 根不同品牌或型号的 C18 色谱柱上,测定银黄口服液中 7 个成分的实际保留时间,并计算标准保留时间（SRT）。借助 DRS Origin 软件,以新绿原酸（峰 1）和黄芩苷（峰 7）为双标化合物,采用双标线性校正法预 测其他 5 个成分的保留时间,并在另一根 C18 色谱柱上进行方法学验证。同时以绿原酸（峰 2）为参照物, 使用相对保留时间法预测其他 6 个成分的保留时间。结果: 双标线性校正法可用于银黄口服液的多组分定性分析,且建立的方法符合方法学验证要求。与相对保留时间法相比,双标线性校正法预测保留时间的准确度更高,色谱柱的适用范围更广。结论: 双标线性校正法能较好地辅助色谱峰定性,方法可行、准确且经 济实用。
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