目的: 建立高效液相色谱法同时测定雪上一枝蒿（短柄乌头）中雷乌宁、雪上一枝蒿庚素、乌头碱和雪上一枝蒿甲素等4个成分含量。方法: 采用RX-C18（250 mm×4.6 mm,5 μm）色谱柱,以甲醇-磷酸盐缓冲溶液（用80%的磷酸调节pH至7.3）（62 ∶ 38）为流动相,流速1 mL · min-1,检测波长210 nm,柱温35 ℃。结果: 雷乌宁在0~1.30 μg（r=0.999 8）范围内,雪上一枝蒿庚素在0~0.64 μg（r=0.999 9）范围内,乌头碱在0~1.94 μg（r=0.999 6）范围内,雪上一枝蒿甲素在0~0.76 μg（r=0.999 7）范围内,均呈现良好的线性关系;4个成分平均回收率分别为98.0%、101.0%、98.0%和103.0%。11批雪上一枝蒿药材样品中雷乌宁含量范围为0.009%~0.261%,雪上一枝蒿庚素含量范围为0.08%~0.46%,乌头碱含量范围为0.10%~0.26%,雪上一枝蒿甲素含量范围为0.17%~0.55%。结论: 该方法简便、准确,重复性好,可用于同时测定雪上一枝蒿中4个生物碱的含量,为更好地控制药材质量提供参考。
吴桃清
,
李华菊
,
陈丽
,
高洁
,
杨孟钰
,
吴宇
,
文建国
,
张杨
,
刘锦
. HPLC法同时测定不同产地雪上一枝蒿中4个生物碱的含量*[J]. 药物分析杂志, 2025
, 45(4)
: 571
-577
.
DOI: 10.16155/j.0254-1793.2024-1155
Objective: To establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of four components (lepenine, songorine, aconitine, and bullatine A) in Aconitum brachypodum Diels. Methods: The analysis was performed on an RX-C18 column (250 mm×4.6 mm, 5 μm) with a mobile phase composed of methanol and phosphate buffer solution (pH adjusted to 7.3 with 80% phosphoric acid) with a ratio of 62 ∶ 38. The detection wavelength was set at 210 nm. The column temperature was 35 ℃ and the flow rate was 1 mL · min-1. Results: Lepenine (0-1.30 μg, r=0.999 8), songorine (0-0.64 μg, r=0.999 9), aconitine (0-1.94 μg, r=0.999 6), and bullatine A (0-0.76 μg, r=0.999 7) all exhibited good linearity. The average recovery rates for the four components were 98.0%, 101.0%, 98.0%, and 103.0%, respectively. The contents of four components in 11 batches of Aconitum brachypodum Diels samples were 0.009%-0.261%(lepenine), 0.08%-0.46%(songorine), 0.1%-0.26%(aconitine), and 0.17%-0.55%(bullatine A). Conclusion: This method is simple, accurate, and reproducible, making it suitable for the simultaneous determination of the four alkaloids in Aconitum brachypodum Diels, which provides a valuable reference for the improved quality control of this herbal material.
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