成分分析

基于UPLC-HRMS的补肺健脾方化学成分分析

  • 刘泽 ,
  • 郑历史 ,
  • 舒胜男 ,
  • 孙淑仃 ,
  • 李荣荣 ,
  • 赵迪 ,
  • 冯素香
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  • 河南中医药大学,郑州 450046
第一作者 Tel:18346407252;E-mail:1753311314@qq.com
* Tel:13526403080;E-mail:fengsx221@163.com

网络出版日期: 2025-10-13

Chemical components analysis of Bufei Jianpi formula based on UPLC-HRMS

  • LIU Ze ,
  • ZHENG Li-shi ,
  • SHU Sheng-nan ,
  • SUN Shu-ding ,
  • LI Rong-rong ,
  • ZHAO Di ,
  • FENG Su-xiang
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  • Henan University of Chinese Medicine, Zhengzhou 450046, China

Online published: 2025-10-13

摘要

目的: 采用超高效液相色谱-高分辨质谱联用(UPLC-HRMS)技术,对补肺健脾方的化学成分进行鉴定分析。方法: 采用Hypersil GOLD(2.1 mm×100 mm,2.6 μm)色谱柱,以甲醇(A)-0.1 %甲酸水(B)为流动相,梯度洗脱,流速为0.2 mL · min-1,柱温30 ℃;质谱数据采集采用正负离子Full MS/dd-MS2扫描模式,通过其特征碎片离子峰信息,采用Compound Discoverer分析数据,结合Chemspider、mzCloud等数据库和已有的相关化学成分信息报道,利用Mass Frontier的裂解推测,结合其裂解规律推断其结构,对补肺健脾方中的化学成分进行分析。结果: 从补肺健脾方中鉴定出化合物221个,主要包括黄酮类化合物65个、苯丙素类化合物40个、萜类化合物21个、生物碱类化合物16个,及其他类化合物79个。结论: UPLC-HRMS可快速鉴定补肺健脾方体化学成分,同时定性分析补肺健脾方剂中的物质基础,可用于补肺健脾方的质量控制。

本文引用格式

刘泽 , 郑历史 , 舒胜男 , 孙淑仃 , 李荣荣 , 赵迪 , 冯素香 . 基于UPLC-HRMS的补肺健脾方化学成分分析[J]. 药物分析杂志, 2025 , 45(5) : 754 -778 . DOI: 10.16155/j.0254-1793.2024-1177

Abstract

Objective: To identify and analyze the chemical components of Bufei Jianpi formula by ultra-high performance liquid chromatography-coupled with high-resolution mass spectrometry (UPLC-HRMS). Methods: A Hypersil GOLD (2.1 mm×100 mm, 2.6 μm) column was used. Methanol (A)-0.1% formic acid water (B) was used as the mobile phase with gradient elution, the flow rate was 0.2 mL · min-1, and the column temperature was 30 ℃.The mass spectrometry data were collected using the Full MS/dd-MS2 scanning mode of positive and negative ions, and the characteristic fragment ion peak information was analyzed by compound discoverer. Combined with Chemspider, mzCloud and other databases and existing reports of relevant chemical composition information, the chemical components of Bufei Jianpi formula was analyzed by using the cracking prediction of Mass Frontier and its cracking rule. Results: A total of 221 compounds were identified from Bufei Jianpi formula, including 65 flavonoids, 40 phenylpropanoids, 21 terpenoids, 16 alkaloids and 79 other compounds. Conclusion: UPLC-Orbitrap Fusion Lumos Tribrid-MS can quickly identify the chemical components of Bufei Jianpi formula and qualitatively analyze the material basis of Bufei Jianpi formula, which can be used for the quality control of BufeiJianpi formula.

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