目的: 建立肉桂叶的HPLC指纹图谱,并结合化学计量学和一测多评法评价不同批次肉桂叶的质量。方法: 采用HPLC法建立了12批肉桂叶的指纹图谱并进行相似度评价,采用聚类分析（CA）和主成分分析（PCA）,对不同批次肉桂叶进行化学模式识别分析。以肉桂酸为内标参照物,建立一测多评（QAMS）法,计算2-羟基肉桂醛、肉桂醇、桂皮醛、邻甲氧基肉桂醛的相对校正因子,采用外标（ESM）法和QASM法测定肉桂叶中2-羟基肉桂醛、肉桂醇、肉桂酸、桂皮醛、邻甲氧基肉桂醛的含量。结果: 指纹图谱共确定 12 个共有峰,通过对照品指认了7个化学成分,除S3与S11批次外,其余批次肉桂叶样品与对照指纹图谱的相似度均>0.9;CA将12批肉桂叶分为2类;PCA显示不同批次间肉桂叶的化学成分存在一定的差异,并确定了3个主成分,累计方差贡献率为 86.022%;QAMS与ESM测定的以上5个化合物含量值无明显差异。结论: 建立的肉桂叶指纹图谱及含量测定方法稳定、可靠,可为肉桂叶的质量控制及评价提供参考。
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