Objective: To establish an HPLC method for simultaneous determination of vancomycin, caffeine and aminophylline in serum of children patients and apply it to clinical detection. Methods: 6% perchloric acid was used as protein precipitator and acetanilide was used as internal standard. The determination was performed on Nucifera C18 column (250 mm×4.6 mm, 5 μm) with mobile phase methanol-0.05 mol · L-1 potassium dihydrogen phosphate (18 ∶ 82, v/v). The detection wavelength was 236 nm, the flow rate was 1 mL · min-1, the column temperature was 25 ℃, and the sample size was 20 μL. Results: The serum concentrations of vancomycin and aminophylline were in the range of 1.0-100 μg · mL-1, and the standard curves were Y=0.010 6X+0.006 9 (r=0.998 8), Y=0.012 7X+0.008 4 (r=0.998 9), respectively. The serum concentration of caffeine had a good linear relationship in the range of 1.0-160 μg · mL-1, and the standard curve was Y=0.015 4X-0.010 1 (r=0.999 1). Quantitative downlines were all 1.0 μg · mL-1. The average recovery rates of the three compounds ranged from 97.8% to 103.1%, and the relative standard deviations of intraday and daytime precision were less than 10%, indicating good sample stability. Ten cases of children receiving vancomycin, aminophylline or caffeine were collected for method verification, which met the needs of clinical detection. Conclusion: The method is simple, rapid, accurate, and is suitable for the simultaneous determination of serum vancomycin, caffeine and aminophylline in children.
LI Sai
,
LI Shou-lin
,
WANG Dong-liang
,
SU Ling-ying
. Simultaneous determination of vancomycin, aminophylline and caffeine in children’s serum by HPLC method*[J]. Chinese Journal of Pharmaceutical Analysis, 2025
, 45(1)
: 92
-98
.
DOI: 10.16155/j.0254-1793.2024-0300
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