Review & Monography

Theoretical and applied of Digital Dancao molecular quantitative detection technology

  • TAN Zhao-yang ,
  • MENG Lei ,
  • LIU Ta-si
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  • 1. School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;
    2. Chenzhou Danya Pharmaceutical Technology Co., Ltd., Chenzhou 423000, China

Received date: 2024-03-11

  Online published: 2025-08-25

Abstract

The present quality control methods of traditional Chinese medicine (TCM), such as morphological identification, microscopic identification, physicochemical analysis, and biological activity testing, cannot fully meet the requirements of TCM quality control. Based on the characteristics of digital PCR technology, a new molecular quantitative analysis technology named Digital Dancao molecular detection technology was established, which used the ratio of specific sequence copy number to internal reference sequence copy number (R value) as the quality control indicator. This technology can quantitatively analyze the adulteration of medicinal herbs, monitor the feeding amount and production process of preparation. It conforms to the clinical characteristics of TCM which using herbal dosage, and can be used for quantitative testing of TCM products from herbs to preparation. Digital Dancao molecular detection technology provides a new solution for the problem of quality monitoring of TCM.

Cite this article

TAN Zhao-yang , MENG Lei , LIU Ta-si . Theoretical and applied of Digital Dancao molecular quantitative detection technology[J]. Chinese Journal of Pharmaceutical Analysis, 2025 , 45(2) : 195 -200 . DOI: 10.16155/j.0254-1793.2024-0159

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