Objective: To establish a quality evaluation method for Chaihu Yujinxiang granules based on fingerprint, multi-component content determination, and chemical pattern recognition, providing important basis for its quality control. Method: The “Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2012 Edition)” was used to establish HPLC fingerprints of 10 batches of Chaihu Yujinxiang granules, identify common peaks, and perform similarity evaluation. HPLC method for determining the content of four active ingredients, namely liquiritin apioside, liquiritin, liquiritigenin, and isoliquiritigenin was established. Cluster analysis and orthogonal partial least squares discriminant analysis were conducted on Chaihu Yujinxiang granules using SPSS 26.0 and SIMCA 14.1 software. Differential components affecting the quality of Chaihu Yujinxiang granules were screened based on the criterion of variable importance projection (VIP) value>1.0. Result: The established fingerprint and multi-component content determination method achieved satisfactory results after methodological investigation. The similarity of the fingerprint of 10 batches of Chaihu Yujinxiang granules ranges from 0.953 to 0.977, with a total of 11 common peaks, 4 of which were identified. The average contents of active ingredients such as liquiritin apioside, liquiritin, liquiritigenin, and isoliquiritigenin in 10 batches of Chaihu Yujinxiang granules were 2.71 mg · g-1, 10.17 mg · g-1, 2.47 mg · g-1, and 1.86 mg · g-1, respectively. The results of cluster analysis and principal component analysis indicates that 10 batches of Chaihu Yujinxiang granules could be clustered into two categories, with S3, S6, and S8 in one category and the rest in another category. The VIP values of peaks 2, 9, 7, and 3 were above 1.0. Conclusion: The established fingerprint and content determination method are stable and reliable. Combined with chemical pattern recognition technology, they can be used to evaluate the overall quality of Chaihu Yujinxiang granules. Peaks 2, 9, 7, and 3 are differential components that affect the quality of Chaihu Yujinxiang granules.
[1] 姜春凤,薛光辉,张金秋,等.基于超高效液相色谱-四极杆飞行时间质谱研究柴胡郁金香颗粒的抗抑郁作用机理[J].分析化学,2023,51(4):579
JIANG CF,XUE GH,ZHANG JQ,et al.Study on the antidepressant mechanism of Chaihu Tulip granules based on ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry[J].Chin J Anal Chem,2023,51(4):579
[2] 姜春凤,薛光辉,付丽娟,等.UPLC-MS/MS法分析柴胡郁金香颗粒中9个药效成分在抑郁症大鼠体内的药代动力学研究[J].质谱学报,2022,43(3):336
JIANG CF,XUE GH,FU LJ,et al.UPLC-MS/MS analysis of the pharmacokinetics of 9 active ingredients in Chaihu Tulip granules in rats with depression[J].J Mass Spectrom,2022,43(3):336
[3] 贺泽慧,范荣,张春虎,等.柴胡总皂苷调控细胞色素P450酶发挥类似柴胡疏肝散抗抑郁作用研究[J].湖南中医药大学学报,2019,39(6):693
HE ZH,FAN R,ZHANG CH,et al.Study on the regulation of cytochrome P450 enzyme by total saponins of Bupleurum chinense and its similar antidepressant effect to Chaihu Shugan san[J].J Hunan Univ Tradit Chin Med,2019,39(6):693
[4] KIM YW,KI SH,LEE JR,et al.Liquiritigenin,an aglycone of liquiritin in Glycyrrhizae Radix,prevents acute liver injuries in rats induced by acetaminophen with or without buthionine sulfoximine[J].Chem Biol Interact,2006,161(2):125
[5] ZHAO Z Y,WANG W X,GUO H Z,et al.Antidepressant-like effect of liquiritin from Glycyrrhiza uralensis in chronic variable stress induced depression model rats[J].Behav Brain Res,2008,194(1):108
[6] ZHAO ZL,PARK SM,GUAN LX,et al.Isoliquiritigenin attenuates oxidative hepatic damage induced by carbon tetrachloride with or without buthionine sulfoximine[J].Chem Biol Interact,2015,225:13
[7] 杨晓宇,苏秀兰.甘草次酸保肝功效的通路作用机制[J].中国组织化学与细胞化学杂志,2019,28(1):66
YANG XY,SU XL.The pathway mechanism of liver protective effect of glycyrrhetinic acid[J].Chin J Biochem Cell Chem,2019,28(1):66
[8] SUN X,DUAN XP,WANG CY,et al.Protective effects of glycyrrhizic acid against non-alcoholic fatty liver disease in mice[J].Eur J Pharmacol,2017,806:75
[9] WANG B,LIAN Y J,DONG X,et al.Glycyrrhizic acid ameliorates the kynurenine pathway in association with its antidepressant effect[J].Behav Brain Res,2018,353:250
[10] 吕昀,程文倩,牛可敬,等.基于多维整合策略的中药复方制剂质量评价研究[J/OL].中草药,(2024-09-11) [2024-11-15].http://kns.cnki.net/kcms/detail/12.1108.R.20240909.0935.002.html
LÜ Y,CHENG WQ,NIU KJ,et al.Study on quality evaluation of traditional Chinese medicine compound preparations based on multidimensional integration strategy [J/OL].Chin Tradit Herb Drugs,(2024-09-11) [2024-11-15].http://kns.cnki.net/kcms/detail/12.1108.R.20240909.0935.002.html
[11] 梁朝锋,韩晓珂,秦亚东,等.栀子饮片及栀子配方颗粒的指纹图谱建立、含量测定及化学模式识别[J].中国药房,2022,33(14):1718
LIANG CF,HAN XK,QIN YD,et al.Establishment of fingerprint spectra,content determination,and chemical pattern recognition of Gardenia jasminoides decoction pieces and Gardenia jasminoides formula granules[J].China Pharm,2022,33(14):1718
[12] 郎天琼,封云倩,简进珍,等.复方胃痛胶囊HPLC指纹图谱建立及5种成分含量测定[J].中药材,2023,46(3):682
LANG TQ,FENG YQ,JIAN JZ,et al.Establishment of HPLC fingerprint and determination of five components in compound stomach pain capsules[J].Chin Med Mater,2023,46(3):682
[13] 李慧峰,孔祥鹏,孟霜,等.复方茴芹颗粒的指纹图谱建立、化学模式识别及多成分含量测定[J].中国药房,2022,33(21):2627
LI HF,KONG XP,MENG S,et al.Establishment of fingerprint spectrum,chemical pattern recognition,and determination of multi-component content of compound anise granules[J].China Pharm,2022,33(21):2627
[14] 王韵旨,郭丽,吴作敏,等.基于指纹图谱及化学模式识别的归芪通脉合剂质量评价[J].中国药房,2022,33(20):2503
WANG YZ,GUO L,WU ZM,et al.Quality evaluation of Guiqi Tongmai mixture based on fingerprint and chemical pattern recognition[J].China Pharm,2022,33(20):2503
[15] 陈文,余本功.一种基于多向量相似度的聚类分析方法研究[J].陇东学院学报,2023,34(2):38
CHEN W,YU BG.Research on a clustering analysis method based on multi vector similarity[J].J Longdong Univ,2023,34(2):38
[16] 李颖,马雨辰,刘萌,等.近红外光谱技术结合偏最小二乘判别分析检测三七品质[J].食品安全质量检测学报,2022,13(12):3923
LI Y,MA YC,LIU M,et al.Detection of Panax notoginseng quality using near-infrared spectroscopy combined with partial least squares discriminant analysis[J].J Food Saf Qual Inspec,2022,13(12):3923
[17] 段雨晴,朱田密,陈树和,等.桦褐孔菌药材的薄层色谱鉴别和HPLC指纹图谱建立及化学模式识别分析[J].中国药房,2023,34(1):52
DUAN YQ,ZHU TM,CHEN SH,et al.Thin layer chromatography identification and HPLC fingerprint establishment,as well as chemical pattern recognition analysis of Botrytis cinerea medicinal materials[J].China Pharm,2023,34(1):52
[18] 中华人民共和国药典20202年版.一部[S].2020:88
ChP 2020.Vol Ⅰ[S].2020:88
[19] 魏冠华. 乌拉尔甘草化学成分及其神经保护活性研究[D].甘肃:兰州大学,2021
WEI GH.Chemical Constituents and Neuroprotective Activity of Ural Licorice [D].Gansu:Lanzhou University,2021
[20] 杨倩倩,孟子盈,王晓禹,等.甘草苷的提取纯化和药理作用研究进展[J].中国野生植物资源,2023,42(4):12
YANG QQ,MENG ZY,WANG XY,et al.Research progress on extraction,purification,and pharmacological effects of glycyrrhizin[J].Chin Wild Plant Resour,2023,42(4):12
[21] MURCK H,KARAILIEV P,KARAILIEVOVA L,et al.Treatment with Glycyrrhiza glabra extract induces anxiolytic effects associated with reduced salt preference and changes in barrier protein gene expression[J].Nutrients,2024,16(4):515
[22] 李葆林,麻景梅,田宇柔,等.甘草中新发现化学成分和药理作用的研究进展[J].中草药,2021,52(8):2438
LI BL,MA JM,TIAN YR,et al.Research progress on newly discovered chemical components and pharmacological effects in licorice[J].Chin Tradit Herb Drugs,2021,52(8):2438
