Objective: To establish a quantitative analysis of multi-components by single-marker (QAMS) method for the simultaneous determination of brucine, strychnine, brucine N-oxide and strychnine N-oxide in Semen Strychni. Methods: The analysis of chloroform reflux extract of Semen Strychni was performed on the Agilent ZORBSX Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm), with the mobile phase consisted of acetonitrile-0.02 mol · L-1 potassium dihydrogen phosphate solution (pH 4) (23 ∶ 77). The flow rate was 1.0 mL · min-1. The column temperature was35 ℃ and the detection wavelength was 260 nm. Strychnine was used as the internal standard. The relative correction factors of strychnine and the N-oxides were calculated, and the contents of the four components were measured separately. Results: Four constituents showed good linear relationship within their own ranges (r≥0.999 8), whose average recoveries were 99.2%-102.3% with RSDs of 0.72%-1.57%. The results obtained by QAMS showed no significant difference compared to the external standard method (EMS) (P>0.05). The contents of brucine, strychnine and their N-oxides were 0.746%-1.759%, 1.39%-3.27%, 0.016 5%-0.056 1%, and 0.035 2%-0.119 0%,respectively. Conclusion: The method is simple, fast, accurate and reliable, and it’s especially suitable for the quality control of the processing technology and processed products of Semen Strychni.
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