Objective: To establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of four components (lepenine, songorine, aconitine, and bullatine A) in Aconitum brachypodum Diels. Methods: The analysis was performed on an RX-C18 column (250 mm×4.6 mm, 5 μm) with a mobile phase composed of methanol and phosphate buffer solution (pH adjusted to 7.3 with 80% phosphoric acid) with a ratio of 62 ∶ 38. The detection wavelength was set at 210 nm. The column temperature was 35 ℃ and the flow rate was 1 mL · min-1. Results: Lepenine (0-1.30 μg, r=0.999 8), songorine (0-0.64 μg, r=0.999 9), aconitine (0-1.94 μg, r=0.999 6), and bullatine A (0-0.76 μg, r=0.999 7) all exhibited good linearity. The average recovery rates for the four components were 98.0%, 101.0%, 98.0%, and 103.0%, respectively. The contents of four components in 11 batches of Aconitum brachypodum Diels samples were 0.009%-0.261%(lepenine), 0.08%-0.46%(songorine), 0.1%-0.26%(aconitine), and 0.17%-0.55%(bullatine A). Conclusion: This method is simple, accurate, and reproducible, making it suitable for the simultaneous determination of the four alkaloids in Aconitum brachypodum Diels, which provides a valuable reference for the improved quality control of this herbal material.
WU Tao-qing
,
LI Hua-ju
,
CHEN Li
,
GAO Jie
,
YANG Meng-yu
,
WU Yu
,
WEN Jian-guo
,
ZHANG Yang
,
LIU Jin
. Simultaneous determination of four alkaloids in Aconitum brachypodum Diels from different habitats by HPLC*[J]. Chinese Journal of Pharmaceutical Analysis, 2025
, 45(4)
: 571
-577
.
DOI: 10.16155/j.0254-1793.2024-1155
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