Objective: To elucidate the differences in the contents of pyrrolizidine alkaloids among different quality grades of Farfarae Flos. Methods: A UPLC-MS/MS method was established for simultaneous determination of three pyrrolizidine alkaloids, senecionine, senecionine N-oxide and senkirkine in Farfarae Flos of different quality grades.An ACQUITY UPLC HSS T3 column (150 mm×2.1 mm, 1.8 μm) was used, themobile phase was 0.5% formic acid water-methanol (80 ∶ 20) with isocratic elution. The MS system was operated by using electrospray ionization (ESI) in the positive ion mode, and the scan mode was in multiple reactions monitoring (MRM) mode. External standard method was used in the experiment. Results: The contents of senecionine in first-grade, second-grade, and ungraded Farfarae Flos were 0.06-0.16 μg · g-1, 0.20-0.49 μg · g-1 and 0.39-1.02 μg · g-1, respectively. The contents of senecionine N-oxide were 0.89-1.66 μg · g-1, 3.61-5.12 μg · g-1 and 4.47-7.87 μg · g-1, respectively. The contents of senkirkine were 46.53-85.00 μg · g-1, 122.95-227.43 μg · g-1 and 153.83-282.59 μg · g-1, respectively. In the flower buds, pedicels and roots of Farfarae Flos, the contents of senecionine were 0.07-0.18 μg · g-1,0.60-1.16 μg · g-1 and 0.22-1.65 μg · g-1, respectively. The contents of senecionine N-oxide were 0.64-1.59 μg · g-1,0.91-9.78 μg · g-1 and 1.58-5.89 μg · g-1, respectively. The contents of senkirkine were 51.35-82.22 μg · g-1, 176.29-246.80 μg · g-1and 321.00-343.30 μg · g-1, respectively. Conclusion: This study reveals the relationships between the content of pyrrolizidine alkaloids in Farfarae Flos and its quality grade and medicinal parts. These results provide new perspective for quality evaluation and standard improvement.
ZAN Ke
,
WU Yun
,
CAI Xi-sheng
,
WANG Zhao
,
WANG Dan-dan
,
JIN Hong-yu
,
LI Hai-liang
. Studies on the content of hepatotoxic pyrrolizidine alkaloids in Farfarae Flos of different quality grades*[J]. Chinese Journal of Pharmaceutical Analysis, 2025
, 45(4)
: 702
-709
.
DOI: 10.16155/j.0254-1793.2024-1032
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