Bioassay

Application of genetic antimicrobial susceptibility testing in the development of selective medium for the enumeration of multi-strain microecological preparation

  • XING Sheng ,
  • FENG Dan-yang ,
  • SHEN Zhen ,
  • HU Wen-hong ,
  • LI Ying ,
  • DING Bo
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  • 1. Shandong Institute for Food and Drug Control, Shandong Food and Drug Safety Testing Engineering Technology Research Center, NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Industrial Technology Foundation Public Service Platform,Jinan 250101, China;
    2. School of Biopharmacy, China Pharmaceutical University, Nanjing 211112, China;
    3. School of Pharmaceutical Sciences Shandong University, Jinan 250012, China;
    4. Hangzhou Yuanda Biopharmaceutical Co., Ltd., Hangzhou 311227 China

Online published: 2025-10-13

Abstract

Objective: To establish a selective culture medium screening method for counting viable bacteria from multi-strain microecological preparation based on gene and metabolic pathway analysis, in order to solve the problem of mutual interference in the measurement of counting viable bacteria. Methods: By analyzing the genomic scale metabolic network, the antibiotic sensitivity and resistance of Bacillus cereus, Enterococcus faecalis, Lactobacillus acidophilus, and Bifidobacterium infantis were quickly predicted. Based on the prediction results, suitable types and concentrations of antibiotics with resistance differences among the four microorganisms were screened and added to the culture medium, effectively inhibiting the growth of interfering microorganisms and ensuring accurate and effective counting results. Simultaneously, conventional drug sensitivity experiments results were used to ensure and compare with this method. Results: In the process of viable counting for multi-strain microecological preparation, this method effectively eliminated the interference of Enterococcus faecalis on the counting of Lactobacillus acidophilus live bacteria and the interference of Lactobacillus acidophilus and Enterococcus faecalis on the counting of Bifidobacterium infant, and the counting results were accurate and reliable of each microorganism. Conclusion: The method has strong foresight and specificity. Genomic analysis and metabolic pathway analysis can effectively predict microbial antibiotic resistance, and experimental design based on the predicted results will greatly accelerate experimental efficiency, making the results more predictive and accurate, and has broad application prospects in selective culture medium screening for counting viable bacteria from multi-strain microecological preparation.

Cite this article

XING Sheng , FENG Dan-yang , SHEN Zhen , HU Wen-hong , LI Ying , DING Bo . Application of genetic antimicrobial susceptibility testing in the development of selective medium for the enumeration of multi-strain microecological preparation[J]. Chinese Journal of Pharmaceutical Analysis, 2025 , 45(5) : 867 -879 . DOI: 10.16155/j.0254-1793.2024-1097

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