Objective: To develop an HPLC method for simultaneous determination of six saponins (notoginsenoside R1, gensinoside Rg1, gensinoside Re, gensinoside Rf, gensinoside Rb1, gensinoside Rd) in Xinbao pills. Methods: The separation was performed on an Avantor Alltima C18 chromatography column (250×4.6 mm, 5 μm) with a gradient elution of acetonitrile and 0.1% formic acid aqueous solution as mobile phases at a flow rate of 1.0 mL · min-1. The detection wavelength was set at 203 nm, and the column temperature was set at 25 ℃. Results: Six saponins showed good linear relationships within their respective ranges (r≥0.999 6), and the precision, stability and repeatability were good. The average sample recoveries were 94.2%~102.0% with the RSD of 0.8% to 2.4%. The contents of notoginsenoside R1, gensinoside Rg1, gensinoside Re, gensinoside Rf, gensinoside Rb1, gensinoside Rd in 11 batches samples were 2.064 4-2.934 9 mg · g-1, 10.685 9-12.053 9 mg · g-1, 2.652 1-3.350 8 mg · g-1, 0.441 1-0.552 5 mg · g-1, 11.700 0-13.637 7 mg · g-1 and 2.976 5-3.817 8 mg · g-1, respectively. Conclusion: The method is accurate, reliable, and reproducible, and can be used for quantitative analysis and quality control of Xinbao pills.
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