Quality Control

Quality evaluation of Folium Cinnamoni based on HPLC fingerprint combined with chemometrics and QAMS*

  • DING Fu-juan ,
  • ZHANG Jing ,
  • YUAN Yan-fei ,
  • WANG Tao ,
  • LIU Yu-ling ,
  • JI Guo-li
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  • Taian Institute for Food and Drug Control, Taian Fiber Inspection Institute, Taian 271000, China

Received date: 2025-01-15

  Online published: 2025-11-13

Abstract

Objective: To establish the HPLC fingerprint and evaluate the quality of different batches of Folium Cinnamoni by chemometrics and quantitative analysis of multi-components with a single-marker (QAMS). Methods: HPLC was used to establish the fingerprints of 12 batches of Folium Cinnamoni and perform similarity evaluation. And then principal component analysis (PCA) and cluster analysis (CA) were used to analyze the chemical pattern of different batches of Folium Cinnamoni. The QAMS method was established by using cinnamic acid as the internal standard reference. The relative correction factors of 2-hydroxy cinnamaldehyde, cinnamyl alcohol, trans-cinnamaldehyde and 2'-methoxy cinnamaldehyde were calculated. The contents of 2-hydroxycinnamaldehyde, cinnamyl alcohol, cinnamic acid, trans-cinnamaldehyde and 2'-methoxy cinnamaldehyde in Folium Cinnamoni were determined by external standard (ESM) method and QAMS method. Results: A total of 12 common peaks were identified from the fingerprint, and 7 of them were confirmed by reference substance. CA divided 12 batches of Folium Cinnamoni into 2 categories; PCA showed that there were some differences in the chemical composition of them. Three principal components were identified, and the result showed that the cumulative variance contribution rate was 86.022%. The contents of these 5 compounds measured by QAMS and ESM showed no significant difference. Conclusion: The fingerprint and content determination method of Folium Cinnamoni are stable and reliable, which can provide reference for quality control and evaluation of Folium Cinnamoni.

Cite this article

DING Fu-juan , ZHANG Jing , YUAN Yan-fei , WANG Tao , LIU Yu-ling , JI Guo-li . Quality evaluation of Folium Cinnamoni based on HPLC fingerprint combined with chemometrics and QAMS*[J]. Chinese Journal of Pharmaceutical Analysis, 2025 , 45(6) : 996 -1004 . DOI: 10.16155/j.0254-1793.2025-0035

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