Objective: To provide an experimental basis of the new fermentation process of multi-strain, based on the previous studies on the strain separation and processing principle of Arisaema Cum Bile, and the dominant strain is screened out and the process of multi-strain fermentation process was further optimized. Methods: Every strain isolated in the previous stage was single fermented to prepare a series of Arisaema Cum Bile. The content of hyocholic acid, hyodeoxycholic acid, and chenodeoxycholic acid was determined using HPLC-ELSD and screened for the advantageous strain. Taking the total amount of free bile acid and the content of taurine, glycine, and bilirubin as the indexes, making the addition amount, fermentation temperature, and fermentation time of the multi-strains as the factors, and combining the orthogonal test and multi-index weighted scoring method, the fermentation process was investigated and the new fermentation process of the multi-strains was determined. Results: The advantageous strains were identified as Lactobacillus plantarum and Enterococcus (anaerobic). A new process of multi-strain fermentation has been screened out: mix with a ratio (1 ∶ 1) of Arisaematis Rhizoma powder and bile containing 10%-15% multi-strains liquid [1 ∶ 1 mixture of Lactobacillus plantarum and Enterococcus (anaerobic)], ferment at 32 ℃ with 80% humidity for 15 days, take out, steam for 1 h to pass thoroughly, cut into diced, and dry in the sun. Conclusion: The optimized fermentation process for Arisaema Cum Bile is stable and feasible, which can improve the quality of the products and provide scientific basis for the standardized production of Arisaema Cum Bile.
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